Methanol

2,7-dichlorofluorescein diacetate (DCFH-DA), 2,2′-Azo-bis-aminopropane (ABAP) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Hanks' Balanced Salt Solution (HBSS), fetal bovine serum (FBS) and Dulbecco's modified Eagle's medium (DMEM), and other cell culture reagents were purchased from Gibco (Invitrogen, Barcelona, Spain). Methyl syringate, epicatechin, syringic acid, catechin, 4-coumaric acid, gallic acid, quercetin, apigenin, luteolin, kaempferol, naringenin, formononetin, genistein, 3-coumaric acid, daidzein, pelargonidin, 2-coumaric acid, biochanin A and cyanidin were purchased from Sigma. Ultrapure water was obtained using a Milli-Q system (Millipore, Bedford, MA, USA) and methanol, acetonitrile and 2-propanol were purchased from Teknokroma (Barcelona, Spain). All other chemicals were of analytical grade.

All reagents used were of highest purity, iodoform (CHI 3 ), 1,2 dibromopropane (used as internal standard SI) and THMs mixture 100 µg/µL of each compound: chloroform (CHCl 3 ), bromodicloromethane (CHBrCl 2 ), dibromochloromethane (CHBr 2 Cl), and bromoform (CHBr 3 ), were purchased from Sigma-Aldrich (Steinhein Germany). Iodo-trihalomethanes (I-THMs): bromodiiodomethane (CHBrI 2 90%), bromochloroiodomethane (CHBrClI 95%), chlorodiiodomethane (CHClI 2 95%), dibromoiodomethane (CHBr 2 I 95%) and dichloroiodomethane (CHCl 2 I 95%) were purchased from Orchid Cellmark (New Westminster, BC, Canada). Methanol, n-hexane, toluene, acetonitrile and acetone were purchased from Teknokroma (Barcelona, Spain). n-Octanol was supplied from Merck (Barcelona, Spain). Accurel Q 3/2 polypropylene hollow fiber membranes with an inner diameter of 600 µm, 200 µm of wall thickness and 0.2 µm pore size was obtained from Wuppertal (Germany). Ultrapure water (18MΩ cm) was obtained from a Milli-Q water-purification system (Millipore, Watford, UK) and was used for spiked samples and blanks preparation. to achieve a final concentration of 5 µg L -1 .