Dialyzed calcein encapsulated polymer/lipid hybrid LUVs were 100-fold diluted with PBS before leakage experiment. 100 µL of the diluted calcein encapsulated samples were added to a 96-well flat black plate (Corning Incorporated, Corning, NY, USA). Fluorescence was measured at 520 nm (excitation at 480 nm) using a fluorescence plate reader (Infinite 200 Pro, Tecan, Salzburg, Austria). 5 µL of 1% Triton X-100 was added for total lysis of the vesicles. In order to estimate the encapsulation of calcein for each formulation, fluorescence of calcein of dialyzed hybrid vesicles as background was subtracted from that of lysed hybrid vesicles with Triton X-100.
96 well flat black plate
Manufactured by Corning
Sourced in United States
The 96-well flat black plate is a laboratory equipment designed for various scientific applications. It features a flat bottom and a black color to reduce light interference and background fluorescence. The plate has 96 individual wells, allowing for multiple samples to be processed simultaneously. The core function of this product is to provide a standardized and consistent platform for a variety of assays and experiments.
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Lab products found in correlation
2 protocols using 96 well flat black plate
1
Calcein Release from Hybrid Vesicles
2
Monitoring Tyrosine Fluorescence in PAP(248-286) Aggregation
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Intrinsic Tyrosine fluorescence of aggregating PAP(248–286) sample was monitored by incubating 2 mg mL−1 of peptide in the absence or presence of AA at various molar ratios (PAP(248–286) : AA – 10 : 1, 5 : 1, 1 : 1, 1 : 5 and 1 : 10) in PBS as mentioned in Section 2.3. At the end of 84 h, samples were thawed to room temperature and transferred to a 96 well flat black plate (Corning). The total volume used for analysis was made up to 100 μL using PBS. Subsequently, the Tyrosine-specific fluorescence intensity was recorded on a microplate reader (Infinite M200, Tecan, Switzerland). The excitation and emission wavelengths were 276 nm and 303 nm, respectively. All measurements were performed in triplicate, and the assay was repeated to ensure reproducibility.
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