700 point scanning confocal microscope

Manufactured by Zeiss

Sourced in Germany

The Zeiss 700-point scanning confocal microscope is a high-performance imaging system designed for advanced microscopy applications. It utilizes a scanning mechanism to capture detailed images by focusing a laser beam on specific points within a sample. The core function of this microscope is to provide high-resolution, three-dimensional imaging capabilities for a wide range of scientific and research purposes.

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Lab products found in correlation

Hoechst 33342, by Thermo Fisher Scientific (4 mentions) Lystroacker red dnd 99, by Thermo Fisher Scientific (2 mentions) Tgn38 antibody, by Novus Biologicals (2 mentions) Mitotracker red cmxros, by Thermo Fisher Scientific (2 mentions) Fv3000 confocal laser scanning microscope, by Olympus (1 mentions)

2 protocols using 700 point scanning confocal microscope

Subcellular Organelle Localization in INS-1 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

INS-1 cells were then fixed in 4% paraformaldehyde for 10 min at room temperature, blocked in 5% goat serum, incubated with Hoechst 33342 (Thermo Fisher Scientific, H3570, Waltham, MA, USA), and mounted with a glass coverslip. For subcellular organelle localization, Lystroacker Red DND-99 (Thermo Fisher Scientific, L7528, Waltham, MA, USA), Mitotracker Red CMXRos (Thermo Fisher Scientific, M7512, Waltham, MA, USA), ACAA1A antibody (Gene Tex, GTX114229, Zeeland, MI, USA), RFP-KDEL plasmid, and TGN38 antibody (Novus, NB300-575, Littleton, CO, USA) were used to detect lysosomes, mitochondria, peroxisomes, the ER, and Golgi, respectively. Images were acquired using a Zeiss 700-point scanning confocal microscope with a 63× lens with oil (Zeiss, Oberkochen, Germany). Images were analyzed by Fiji software. During analysis, single z stacks were processed, and images from different channels were merged. For PLISH assays, stack images were taken using the 60× lens of Olympus microscopy (FV3000, confocal laser scanning microscope).

Li M., Shao F., Qian Q., Yu W., Zhang Z., Chen B., Su D., Guo Y., Phan A.V., Song L.S., Stephens S.B., Sebag J., Imai Y., Yang L, & Cao H. (2021). A putative long noncoding RNA-encoded micropeptide maintains cellular homeostasis in pancreatic β cells. Molecular Therapy. Nucleic Acids, 26, 307-320.

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Subcellular Organelle Localization in INS-1 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

INS-1 cells were then fixed in 4% paraformaldehyde for 10min at room temperature, blocked in 5% goat serum, incubated with Hoechst33342 (Thermo Fisher Scientific, H3570), and mounted with a glass coverslip. For subcellular organelle localization, Lystroacker™ Red DND-99 (Thermo Fisher Scientific, L7528), Mitotracker™ Red CMXRos (Thermo Fisher Scientific, M7512), ACAA1 antibody (Gene Tex, GTX114229), RFP-KDEL plasmid, and TGN38 antibody (Novus, NB300-575) were used to detect lysosomes, mitochondria, peroxisomes, the ER, and Golgi respectively. Images were acquired using a Zeiss 700-point scanning confocal microscope with a 63x lens with oil (Zeiss, Oberkochen, Germany).
Images were analyzed by Fiji software. During analysis, single Z-stacks were processed and images from was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint (which this version posted July 26, 2020. ; https://doi.org/10.1101/2020.05.12.091728 doi: bioRxiv preprint different channels were merged. For PLISH assays, stack images were taken using the 60x lens of Olympus microscopy (FV3000, confocal laser scanning microscope).

Li M., Shao F., Qian Q., Yu W., Zhang Z., Chen B., Su D., Guo Y., Phan A., Song L., Stephens S.B., Sebag J.A., Imai Y., Yang L., & Cao H. (2020). A putative long noncoding RNA-encoded micropeptide maintains cellular homeostasis in pancreatic β-cells.

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