Suz12 antibody

The short and long RNA products were synthesized using a TranscriptAid T7 High Yield Transcription Kit (Thermo Fisher, #K0441). The RNAs were labeled with biotin using a Pierce™ RNA 3ʹ End Desthiobiotinylation Kit (Thermo Fisher, #20163) and then purified with a GeneJET RNA Purification Kit (Thermo Fisher, #K0731). The lncRNAs pull down assays were conducted using a Pierce™ Magnetic RNA–Protein Pull-Down Kit (Thermo Fisher #20164) following the manufacturer’s instructions. The lysate of Hela cells was incubated with biotin labeled RNAs. The proteins were analyzed with a Western Blot using SUZ12 antibody (Cell Signaling Technology, #3737).

All immunoblotting analyses were performed following SDS PAGE using standard methods. Antibodies used for immunoblotting were HIF1α antibody (Abcam, CAT# ab51608, dilution 1:1000), HIF1α antibody (Cell Signaling Technology, CAT# 79233, dilution 1:1000), EZH2 antibody (Cell Signaling Technology, CAT# 5246, dilution 1:1000), SUZ12 antibody (Cell Signaling Technology, CAT# 3737, dilution 1:1000), EED antibody (Millipore, CAT# 09-744, dilution 1:1000), HDAC1 antibody (Cell Signaling Technology, CAT# 34589, dilution 1:1000), HDAC2 antibody (Cell Signaling Technology, CAT# 57156, dilution 1:1000), phosphorylated-STAT1 antibody (Cell Signaling Technology, CAT# 9167, dilution 1:1000), STAT1 antibody (Cell Signaling Technology, CAT# 9176, dilution 1:1000), PD-L1 antibody (Cell Signaling Technology, CAT# 3684, dilution 1:1000), IRF1 antibody (Abcam, CAT# ab26109, dilution 1:1000), H3K27me3 antibody (Cell Signaling Technology, CAT# 9733, dilution 1:1000), H3K27ac antibody (Abcam, CAT# 4729, dilution 1:1000), and GAPDH antibody (Cell Signaling Technology, CAT# 2118, dilution 1:1000).