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2 protocols using supelco 37 comp fame mix

1

Fatty Acid Methyl Ester Analysis

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Hydrochloric acid, potassium chloride, sodium sulfate anhydrous, methanol, hexane, chloroform and sodium methoxide solution were purchased from Sigma-Aldrich, (Sigma-Aldrich, Burlington, MA, USA) [49 (link)]. Supelco 37 comp. FAME mix was purchased from Supelco (Supelco Analytical, Bellefonte, PA, USA).
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2

Detailed Chemical Reagent Protocol

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Sulfuric acid, hydrochloric acid, formic acid, perchloric acid, sodium nitrate, sodium hydroxide, potassium hydroxide, potassium chloride, sodium sulfate anhydrous, potassium dihydrogen phosphate, chloramine T trihydrate, trichloroacetic acid, 2-thiobarbituric acid, ethylenediaminetetraacetic acid (EDTA), propylgallate, 4-(dimethylamino)benzaldehyde, petroleum ether (b.p. 40–60 °C), methanol, ethanol, hexane, acetonitrile, 2-propanol, chloroform, sodium methoxide solution, amino acid standard, L-tryptophan, hydroxyproline, L-2-aminobutyric acid, and 1,1,3,3-tetraehoxypropane were purchased from Sigma-Aldrich (Sigma-Aldrich Co., St. Louis, MO, USA).
Supelco 37 comp. FAME mix, linoleic acid methyl ester isomer mix were purchased from Supelco (Supelco Analytical, Bellefonte, PA, USA), trans FAME mix K110 from Grace (Grace, Deerfield, IL, USA).
AccQ Fluor reagent kit and AccQ Tag eluent A concentrate was purchased from Waters (Waters Corp., Miliford, MA, USA). Boric acid was purchased from AFT (Bratislava, Slovakia), Kjeltabs from Velf Scientifica (Velf Scientifica srl, Usmate, Italy).
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