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Epc 10

Manufactured by HEKA Elektronik
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Sourced in Germany, United States

The EPC-10 is a programmable voltage-clamp amplifier designed for electrophysiology research. It provides precise control and measurement of membrane potential and current in biological cells and tissues.

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Lab products found in correlation

Patchmaster software, by HEKA Elektronik (8 mentions) Patchmaster, by HEKA Elektronik (7 mentions) Pulse software, by HEKA Elektronik (3 mentions) Fitmaster, by HEKA Elektronik (3 mentions) Axopatch 200b, by Molecular Devices (1 mentions) Sodium hypochlorite solution, by Merck Group (1 mentions) Epc 10 amplifier, by HEKA Elektronik (1 mentions) Bx51wi, by Olympus (1 mentions) 1.5 mm borosilicate glass capillaries, by Sutter Instruments (1 mentions) Pulse 8, by HEKA Elektronik (1 mentions) Patchmaster, by Wavemetrics (1 mentions) Igor pro, by Wavemetrics (1 mentions) Pc 10, by Narishige (1 mentions) Pc 10 puller, by Narishige (1 mentions) Gc150f 10, by Harvard Apparatus (1 mentions) Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions) Borosilicate capillaries, by Sutter Instruments (1 mentions) Rc 27, by Warner Instruments (1 mentions) Pv830, by World Precision Instruments (1 mentions)

Spelling variants of this product

EPC-10 amplifier (119 citations) EPC-10 patch-clamp amplifier (37 citations) HEKA EPC-10 amplifier (17 citations) EPC 10 USB Patch Clamp Amplifier (8 citations) Double EPC-10 amplifier (5 citations) EPC-10 Quadro patch-clamp amplifiers (2 citations) HEKA EPC-10 (2 citations) HEKA EPC 10 Patch Clamp Amplifier (2 citations) EPC 10 USB patch (2 citations) EPC-9/EPC-10 amplifiers (2 citations)

38 protocols using epc 10

1

Patch-Clamp Recordings of ND7/23 Cells

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Transfected ND7/23 cells were recorded using an EPC 10 USB patch-clamp amplifier (HEKA Electronics, Lambrecht, Germany). The sampling rate was 50 kHz with a 10 kHz low-pass Bessel filter. Patch pipettes were pulled using a DMZ pipette puller (Zeitz-Instrumente Vertriebs GmbH, Martinsried, Germany). Pipettes with a tip resistance in the range of 0.9 to 2.2 MΩ were used. Only green fluorescent cells were chosen for patch-clamp recordings. Experiments were performed at room temperature Series resistance compensation was 70-80% and only cells with a series resistance of less than 5.5 MΩ throughout the recordings were included in the analysis.
The P/4 procedure was used for online leak current subtraction. After establishing the whole-cell configuration, cells were held at -120 mV and stimulated to 0 mV with a frequency of 0.1 Hz for 5 min to stabilize sodium currents.
Heinrichs B., Liu B., Zhang J., Meents J., Le K., Hautvast P., Zhu X., Li N., Liu Y., Rothermel M., Namer B., Zhang X., Lampert A., & Duan G. (2020). The Potential Effect of Nav1.8 in Autism Spectrum Disorder: Evidence from A Congenital Case with Compound Heterozygous SCN10A Mutations.
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2

Single Nanopore Electrical Characterization

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Electrical measurements were performed using a patch-clamp amplifier (EPC10 HEKA electronics, Germany). The current is measured by Ag/AgCl electrode. A single nanopore was mounted in the chemical Teflon cell containing an electrolyte solution. One electrode was plugged to the working end of the amplifier (trans chamber, base side) and the other electrode connected to the ground (cis chamber, tip side). Recorded currents were analyzed by Fitmaster (Heka Elektronik, Germany).
For I-V curves, the currents data were recorded as a function of the time under constant voltage from -1
V to 1 V by 100 mV step and from -100 mV to 100 mV by 10 mV steps. All current traces were recorded during 10 s at a frequency of 50 kHz. These measurements were performed 3 times. The conductance G is extracted from the linear zone of I-V curves from typically -75 mV to 75 mV.
Ma T., Gaigalas P., Lepoitevin M., Plikusiene I., Bechelany M., Janot J.M., Balanzat E, & Balme S. (2018). Impact of Polyelectrolyte Multilayers on the Ionic Current Rectification of Conical Nanopores. Langmuir : the ACS journal of surfaces and colloids, 34(11).
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3

Nanopore Sensing of Tau Protein Aggregation

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The nanopore functionalized with PEG moieties was mounted between two compartments of a Teflon cells filled with buffered 1 M KCl aqueous solution (pH = 5.6). The tau187WT or tau187P301L at different aggregation times (from 0 to 376 min) was added to the base side to reach a concentration of 8.2 nM (monomer equivalent). The resistive pulse experiments were performed using a patch-clamp amplifier (EPC 10, HEKA electronics, Germany) with Ag/ AgCl electrodes. A voltage of -200 mV was applied to the working electrode located in the tip side compartment to drive the positively charged protein tau, (isoelectric point of 8.24 44 (link) ) to pass through the nanopore from the base to tip side. The ionic currents were recorded at 20 kHz. The signal filtered at 2.9 kHz by a Bessel filter. The resistive pulse detection was recorded on fly during 1 minute at least 10 times.The current traces were further analyzed to detect events using lab-made software "Peak Nano Tools" developed using Labview. First the signal was filtered using Bessel filter 1kHz. The threshold for the event detection was defined as follow: (i) correct the baseline using Stavinsky-Golay filter (ii), define the noise levels by the global standard deviation methods (iii) define the threshold. In this work, the threshold has been fixed at about 5σ.
Giamblanco N., Fichou Y., Janot J.M., Balanzat E., Han S, & Balme S. (2020). Mechanisms of Heparin-Induced Tau Aggregation Revealed by a Single Nanopore. ACS sensors, 5(4).
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4

Hippocampal Field Potential Recording

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Excitatory field potentials were recorded at room temperature in freshly prepared brain slices that were placed in a recording chamber mounted on an upright 2-photon microscope (see above). Stimulation and recording electrodes (impedance 1 MOhm) were placed in the hippocampal regions CA3 and CA1, respectively. The two electrodes were between 410-560 mm apart. fEPSPs were reliably obtained by the application of a single train of 100 pulses at a frequency of 100 Hz. Amplifier (EPC9 or EPC10, HEKA Electronics) and TIDA 5.25 (Heka electronics) were used to apply the electrical stimulation and to record the postsynaptic potentials.
Logiacco F., Xia P., Georgiev S.V., Franconi C., Chang Y.J., Ugursu B., Sporbert A., Kühn R., Kettenmann H, & Semtner M. (2021). Microglia sense neuronal activity via GABA in the early postnatal hippocampus. Cell reports, 37(13).
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5

Patch-Clamp Analysis of Rat Atrial Myocytes

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Membrane currents of rat atrial myocytes were recorded in the whole-cell configuration of the patch-clamp technique by a patch-clamp amplifier (EPC10, HEKA Electronics, Lambrecht, Germany) at room temperature. Data were reproduced, low-pass-filtered at 1 kHz (−3 dB) by an eight-pole Bessel filter, sampled at 5 kHz, and analysed offline with PatchMaster (HEKA Electronics) and FitMaster (HEKA Electronics). The bath solution contained (mm): 115 NaCl, 20 KCl, 1.8 CaCl2, 0.53 MgCl2, 5.5 glucose and 5.5 Hepes; pH 7.4 with NaOH. The pipette solution contained (mm): 150 KCl, 5 EGTA, 1 MgCl2, 3 K2ATP, 0.1 Na2GTP and 5 Hepes; pH 7.3 with KOH. The tip resistance of the glass electrodes was 2–5 MΩ when filled with the pipette solution.
Chen I.S., Furutani K., Inanobe A, & Kurachi Y. (2014). RGS4 regulates partial agonism of the M2 muscarinic receptor-activated K+ currents. The Journal of Physiology, 592(Pt 6), 1237-1248.
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6

Patch-clamp Characterization of ClC-5 and Barttin

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Whole-cell patch-clamp recordings were performed using either an Axopatch 200B (Molecular Devices, Sunnyvale, CA, United States) or EPC10 (HEKA Electronics, Germany). Borosilicate pipettes (ALA Scientific) with resistances of 1–2 MΩ were pulled on an automated puller (Sutter) and fire-polished. Capacitive cancellation and series resistance compensation were applied to reduce capacitive artifacts and series resistance errors, resulting in voltage errors not exceeding 5 mV. Currents were digitized at 50 kHz sampling rate after analog filtering at 3–10 kHz with a low-pass Bessel filter. The standard extracellular solution contained (in mM): NaCl 145, Hepes 15, KCl 4, CaCl2 2, MgCl2 1, pH 7.4. The standard intracellular solution contained (in mM): NaCl 105, Hepes 15, MgCl2 2, EGTA 5, pH 7.4. P/4 leak subtraction was performed by applying repeating voltage steps with a -60-mV baseline to minimize capacitance artifacts. For electrophysiological characterization of the effects of barttin on ClC-5 function, only cells with higher mCFP (barttin) than YFP (ClC-5) fluorescence intensity were used.
Wojciechowski D., Kovalchuk E., Yu L., Tan H., Fahlke C., Stölting G, & Alekov A.K. (2018). Barttin Regulates the Subcellular Localization and Posttranslational Modification of Human Cl-/H+ Antiporter ClC-5. Frontiers in Physiology, 9, 1490.
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7

Voltage-Clamp Recording of Potassium Currents

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Potassium currents were recorded under voltage-clamp with an EPC10 patch clamp amplifier interface (HEKA Electronics, Lambrecht, Pfalz, Germany). For data acquisition, pulse generation, and analysis, a computer (Dell, Round Rock, TX, USA) running PULSE software (HEKA Electronics) was used. All experiments were conducted at room temperature (20–24 °C). Data are presented as means ± SE of three experiments. The patch pipettes were made from Corning type 7052 glass (Garner Glass, Claremont, CA, USA). Whole-cell currents were obtained conventionally from dialyzed cells voltage-clamped through ruptured membrane patches. The solution bathing the cells contained: 140 mM NaCl, 10 mM HEPES, 1 mM CaCl2, 1 mM MgCl2, 5 mM KCl, and 10 mM glucose, having the pH adjusted to 7.4 saline, and was superfused at 1 mL/min. The pipette solution contained 140 mM KCl, 2 mM MgCl2, 1 mM CaCl2, 10 mM HEPES, and 2.5 mM EGTA (~50 nM free Ca2+), with the pH adjusted to 7.2. The pulse protocol was run a minimum of 10 times before the perfusion with (+)-strebloside to ensure that the currents were stable.
Chen W.L., Ren Y., Ren J., Erxleben C., Johnson M.E., Gentile S., Kinghorn A.D., Swanson S.M, & Burdette J.E. (2017). (+)-Strebloside-induced Cytotoxicity in Ovarian Cancer Cells is Mediated through Cardiac Glycoside Signaling Networks. Journal of natural products, 80(3), 659-669.
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8

Whole-cell Na+ Currents in Pancreatic Islet Cells

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Whole-cell Na+ currents were recorded in α- and β-cells within intact islets using the standard whole-cell configuration as previously described (Gopel et al. 1999 (link)). The measurements were performed using EPC-9 or EPC-10 patch-clamp amplifiers and Pulse software (HEKA Electronics, Lambrecht/Pfalz, Germany). Currents were compensated for capacitive transients and linear leak using a −P/4 protocol. The currents were filtered at 2.9 kHz and digitised at >10 kHz.
The standard extracellular medium for the electrophysiological measurements consisted of (mm) 118 NaCl, 20 tetraethylammonium-Cl (TEA-Cl), 5.6 KCl, 1.2 MgCl2, 5 Hepes (pH 7.4 with NaOH), 2.6 CaCl2, 5 d-glucose and 2 CoCl2 (to block Ca2+ channels). The pipette solution contained (mm) 120 CsCl, 1 MgCl2.6H2O, 1 CaCl2, 10 EGTA, 10 Hepes (pH 7.15 with CsOH) and 3 Mg-ATP. TTX (Alomone Labs, Jerusalem, Israel) was used at a final concentration of 0.1 μg ml−1.
Membrane potential recordings were performed as described previously (De Marinis et al. 2010 (link)) using the perforated-patch technique and K2SO4-filled electrodes. In these experiments, the extracellular (Krebs–Ringer buffer, KRB) solution consisted of (mm) 140 NaCl, 3.6 KCl, 0.5 MgSO4, 0.5 NaH2PO4, 2 NaHCO3, 5 Hepes, 1.5 CaCl2 and glucose as indicated. All electrophysiological experiments were performed at 34°C.
Zhang Q., Chibalina M.V., Bengtsson M., Groschner L.N., Ramracheya R., Rorsman N.J., Leiss V., Nassar M.A., Welling A., Gribble F.M., Reimann F., Hofmann F., Wood J.N., Ashcroft F.M, & Rorsman P. (2014). Na+ current properties in islet α- and β-cells reflect cell-specific Scn3a and Scn9a expression. The Journal of Physiology, 592(Pt 21), 4677-4696.
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9

Electrophysiological Recordings of Islet Cells

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Islets isolated from RIPCre+/−ChR2‐YFP+/−, non‐ChR2‐expressing littermate controls and SST‐RFP mice were also used for patch‐clamp electrophysiological recordings. These recordings (in intact islets) were performed at 33–34°C using an EPC‐10 patch‐clamp amplifier and PatchMaster software (HEKA Electronics, Lambrecht/Pfalz, Germany). Currents were filtered at 2.9 kHz and digitized at >10 kHz. A new islet was used for each recording.
Briant L.J., Reinbothe T.M., Spiliotis I., Miranda C., Rodriguez B, & Rorsman P. (2017). δ‐cells and β‐cells are electrically coupled and regulate α‐cell activity via somatostatin. The Journal of Physiology, 596(2), 197-215.
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10

Whole-cell patch-clamp electrophysiology

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Electrophysiological measurements were performed using the whole‐cell configuration of the patch‐clamp technique with the HEKA amplifier (EPC 10, HEKA Electronics, Reutlingen, Germany), as we had previously published [28 (link)]. GC cells were plated on cover slips and then gently moved to a small chamber under an inverted microscope (Nikon Microscope ECLIPSE FN1, Nikon, Tokyo, Japan). The glass pipette electrodes (Warner Instruments, Hammed, CT, USA), which were connected to the amplifier for amplifying membrane currents and potentials, were applied to orientate gastric cells by using a 3D‐micromanipullator (MP‐225, Sutter Instruments Company, CA, USA). After rupturing cell membrane and achieving whole‐cell configuration, the voltage pulses were employed, starting from ‐100 mV to +60 mV in 10‐mV increments with a duration of 100 ms. Signals were measured by the HEKA amplifier, and data were acquired by the Patchmaster software (HEKA Electronics, Reutlingen, Germany). All whole‐cell recordings were utilized at least 10 min after rupturing cell membrane.
Pan C., Wu J., Zheng S., Sun H., Fang Y., Huang Z., Shi M., Liang L., Bin J., Liao Y., Chen J, & Liao W. (2021). Depression accelerates gastric cancer invasion and metastasis by inducing a neuroendocrine phenotype via the catecholamine/β2‐AR/MACC1 axis. Cancer Communications, 41(10), 1049-1070.
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