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1 myristoyl 2 hydroxy sn glycero phosphocholine

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1-myristoyl-2-hydroxy-sn-glycero-phosphocholine is a synthetic phospholipid compound. It is a precursor molecule used in the study of cell membrane structures and dynamics.

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Lab products found in correlation

Cy5.5 nhs ester, by GE Healthcare (2 mentions) Cy5.5 dmpe, by GE Healthcare (2 mentions) 1 2 dimyristoyl sn glycero 3 phosphatidylcholine, by Avanti Polar Lipids (2 mentions) Gadolinium dtpa dmpe, by Avanti Polar Lipids (2 mentions)

2 protocols using 1 myristoyl 2 hydroxy sn glycero phosphocholine

1

Synthesis of Simvastatin-Loaded rHDL Nanoparticles

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The synthesis of [S]-rHDL was modified from a published method32 . Briefly, simvastatin (AKscentific), 1-myristoyl-2-hydroxy-sn-glycero-phosphocholine (MHPC) and 1, 2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC) (both purchased from Avanti Polar Lipids) were dissolved in chloroform/methanol (4:1 by volume) solvent and dried to form thin film. Human apolipoprotein A1 proteins (ApoA-1) (CSL, Parkville, Australia) dissolved in PBS was added to the film and the solution was incubated in 37°C till the film was hydrated and a homogenous solution was formed. The solution was sonicated to form small [S]-rHDL nanoparticles. Aggregates were removed by centrifugation and filtration. Gadolinium-DTPA-DMPE (Avanti Polar Lipids), Cy5.5-DMPE (DMPE was conjugated with Cy5.5 NHS-ester purchased from GE healthcare), DiR or DiO (Invitrogen) were added when the nanoparticles were subsequently used for imaging purposes. Control rHDL nanoparticles without simvastatin were synthesized with the same procedures.
Duivenvoorden R., Tang J., Cormode D.P., Mieszawska A.J., Izquierdo-Garcia D., Ozcan C., Otten M.J., Zaidi N., Lobatto M.E., van Rijs S.M., Priem B., Kuan E.L., Martel C., Hewing B., Sager H., Nahrendorf M., Randolph G.J., Stroes E.S., Fuster V., Fisher E.A., Fayad Z.A, & Mulder W.J. (2014). A Statin-Loaded Reconstituted High-Density Lipoprotein Nanoparticle Inhibits Atherosclerotic Plaque Inflammation. Nature communications, 5, 3065.
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2

Synthesis of Simvastatin-Loaded rHDL Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols
The synthesis of [S]-rHDL was modified from a published method32 . Briefly, simvastatin (AKscentific), 1-myristoyl-2-hydroxy-sn-glycero-phosphocholine (MHPC) and 1, 2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC) (both purchased from Avanti Polar Lipids) were dissolved in chloroform/methanol (4:1 by volume) solvent and dried to form thin film. Human apolipoprotein A1 proteins (ApoA-1) (CSL, Parkville, Australia) dissolved in PBS was added to the film and the solution was incubated in 37°C till the film was hydrated and a homogenous solution was formed. The solution was sonicated to form small [S]-rHDL nanoparticles. Aggregates were removed by centrifugation and filtration. Gadolinium-DTPA-DMPE (Avanti Polar Lipids), Cy5.5-DMPE (DMPE was conjugated with Cy5.5 NHS-ester purchased from GE healthcare), DiR or DiO (Invitrogen) were added when the nanoparticles were subsequently used for imaging purposes. Control rHDL nanoparticles without simvastatin were synthesized with the same procedures.
Duivenvoorden R., Tang J., Cormode D.P., Mieszawska A.J., Izquierdo-Garcia D., Ozcan C., Otten M.J., Zaidi N., Lobatto M.E., van Rijs S.M., Priem B., Kuan E.L., Martel C., Hewing B., Sager H., Nahrendorf M., Randolph G.J., Stroes E.S., Fuster V., Fisher E.A., Fayad Z.A, & Mulder W.J. (2014). A Statin-Loaded Reconstituted High-Density Lipoprotein Nanoparticle Inhibits Atherosclerotic Plaque Inflammation. Nature communications, 5, 3065.
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