Two-photon imaging was performed using a resonant scanner B scope (Thorlabs) with a Chameleon Ultra II laser (Coherent) and a 20× water immersion objective (1.0 NA, Olympus). Intravascular FITC-dextran and red microspheres were excited at a 820 nm wavelength and images were acquired at 30 Hz (ThorSync software) simultaneously with physiological recordings (3 kHz, ThorSync 9 of 13 software), as previously described (Mestre et al., 2018b) . To visualize the vasculature, fluorescein isothiocyanate-dextran (FITC-dextran, 2,000 kDa) was injected intravenously via the femoral vein immediately before imaging. Segments of the middle cerebral artery were distinguished on the basis of morphology: surface arteries passing superficially to surface veins and exhibiting less branching at superficial cortical depths. ECG and respiratory rate were acquired at 1 kHz and 250 Hz, respectively, using a small-animal physiological monitoring device (Harvard Apparatus). The signals were digitized and recorded with a DigiData 1550A digitizer and AxoScope software (Axon Instruments).
Small animal physiological monitoring device
Manufactured by Harvard Apparatus
The Small-animal physiological monitoring device is a lab equipment designed to monitor various physiological parameters of small animals during experiments or studies. The device is capable of recording and displaying real-time data on the animal's vital signs, such as heart rate, respiration, and temperature.
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2 protocols using small animal physiological monitoring device
1
Two-Photon Imaging of Cerebral Vasculature
2
Multimodal Physiological Monitoring in Rodents
Check if the same lab product or an alternative is used in the 5 most similar protocols
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ECG and respiratory rate were acquired at 1 kHz and 250 Hz, respectively, using a small animal physiological monitoring device (Harvard Apparatus). Arterial blood pressure was measured through an arterial catheter placed in the femoral artery and connected to a pressure transducer and monitor (World Precision Instruments). The signals were digitized and recorded with a DigiData 1550A digitizer and AxoScope software (Axon Instruments).
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