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Nci h1975

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NCI–H1975 is a non-small cell lung cancer cell line derived from a human lung adenocarcinoma. It is a commonly used in vitro model for cancer research.

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Lab products found in correlation

Beas 2b, by iCell Bioscience (2 mentions) Rpmi 1640, by iCell Bioscience (1 mentions) Nci h1299, by iCell Bioscience (1 mentions) Beas 2b, by Thermo Fisher Scientific (1 mentions)

2 protocols using nci h1975

1

Cultivation of Normal and NSCLC Cells

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Normal human lung epithelial cells BEAS-2B (iCell-h023) and NSCLC cell lines A549 (iCell-h011), NCI–H1299 (iCell-h153), NCI–H1650 (iCell-h155-001b), and NCI–H1975 (iCell-h156) were obtained from iCell (Shanghai, China). They were cultivated in Dulbecco's modification of Eagle's medium Dulbecco (DMEM) (iCell-0001), F–12K (iCell-0007), or RPMI-1640 (iCell-0002) media supplemented with 10% phosphate buffer saline (PBS) and 100 U/mL penicillin-streptomycin.
Li W.H., Dang Y., Zhang L., Zhou J.C., Zhai H.Y., Yang Z., Ma K, & Wang Z.Z. (2024). METTL3-mediated m6A methylation of DNMT1 promotes the progression of non-small cell lung cancer by regulating the DNA methylation of FOXO3a. Heliyon, 10(7), e28618.
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2

Culturing Lung Cancer and Normal Cell Lines

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Human normal lung epithelial cell-line BEAS-2B and 4 LUAD cell lines (NCI-H1975; NCI-H441; PC-9; A549) were obtained from Shanghai iCell Bioscience Inc. BEAS-2B were seeded in DMEM medium supplemented with 10% fetal bovine serum (FBS, Gibco, Waltham, MA) and 1% Pen/Strep solution. NCI-H1975, NCI-H441 and PC-9 cells were cultivated in RPMI 1640 medium with 10% FBS and 1% Pen/Strep solution. A549 cells were cultivated in F-12 K medium with 10% FBS and 1% Pen/Strep solution. All above cells were incubated in a 5% CO2 humidified environment under 37°C.
Li C., Fu Y., He Y., Huang N., Yue J., Miao Y., Lv J., Xiao Y., Deng R., Zhang C, & Huang M. (2023). Knockdown of LINC00511 enhances radiosensitivity of lung adenocarcinoma via regulating miR-497-5p/SMAD3. Cancer Biology & Therapy, 24(1), 2165896.
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