MG132 (Sigma-Aldrich)) was dissolved in DMSO to yield a 10 mM stock, then stored at −20° C until use. Doxycycline (Dox) (BD Biosciences) was dissolved in PBS to a 1 mg/ml stock. α-Flag agarose, α-Flag-HRP conjugated antibodies and α-β-actin were obtained from Sigma-Aldrich. α-VEGFR-1 antibodies were purchased from Epitomics, α-E6 N-terminus antibodies were obtained from Euromedex (France), α-E-cadherin was purchased from Cell Signalling Technology, α-caspase 8 antibodies were purchased from BD Biosciences, α-HA was obtained from Roche Applied Science, and secondary ImmunoPure Antibody HRP conjugated antibodies were obtained from Fisher Scientific. α-p53 p122 antibodies were purified from conditioned media obtained during hybridoma growth.
α e cadherin
Manufactured by Cell Signaling Technology
Sourced in United States
α-E-cadherin is an antibody product that recognizes the extracellular domain of E-cadherin. E-cadherin is a transmembrane glycoprotein that mediates calcium-dependent cell-cell adhesion and plays a crucial role in the maintenance of epithelial tissue architecture and cell polarity.
Automatically generated - may contain errors
Lab products found in correlation
α axin, by Cell Signaling Technology (2 mentions)
α cd44, by Cell Signaling Technology (2 mentions)
α hif 1a, by Cell Signaling Technology (2 mentions)
α glut1, by Cell Signaling Technology (2 mentions)
α vegfa, by Abcam (2 mentions)
α β actin, by Abcam (2 mentions)
α β catenin, by Cell Signaling Technology (2 mentions)
α c myc, by Cell Signaling Technology (2 mentions)
α cyclin d1, by Cell Signaling Technology (2 mentions)
α histone h3, by Cell Signaling Technology (2 mentions)
Ne per nuclear and cytoplasmic extraction kit, by Thermo Fisher Scientific (2 mentions)
Ripa buffer, by Beyotime (2 mentions)
α β actin, by Merck Group (1 mentions)
α flag agarose, by Merck Group (1 mentions)
Doxycycline dox, by BD (1 mentions)
Mg132, by Merck Group (1 mentions)
α gapdh, by Merck Group (1 mentions)
α smooth muscle actin sma, by Merck Group (1 mentions)
4 protocols using α e cadherin
1
MG132 and Doxycycline Reagents Protocol
2
3D Spheroid Characterization by Western Blot
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3D spheroids (n = 8) were collected by floating the wells with 50 μL PBS. Samples were then centrifuged at 6000 RCF for 5 min and cell pellets shock-frozen in liquid nitrogen. Whole cell lysates from 2D and 3D cultures were generated using Tris Glycine SDS sample buffer (Gradipore, Frenchs Forest, NSW, Australia) by shaking at room temperature for 1 h and further processed via SDS-PAGE as described previously [36 (link)]. The following primary antibodies were used: α-AR (1:250; PG-21, Millipore, Darmstadt, Germany), α-vimentin (1:500, Dako, Vienna, Austria), α-pAkt (Ser475, 1:1000, Cell Signaling, Danvers, MA, USA), α-E-cadherin (1:1000, Cell Signaling), α-smooth muscle actin (SMA, 1:500, Sigma), α-GAPDH (1:50,000; Millipore).
3
Protein Expression Analysis in Breast Cancer
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The whole cell proteins of MDA-MB-231/MDA-MB-468 cells were extracted from cells using RIPA buffer (Beyotime, Shanghai, China) and the nuclear extracts were prepared with a NE-PER Nuclear and Cytoplasmic Extraction Kit (Pierce Biotechnology, Rockford, USA). Western blots were probed with α-phospho LRP6 (Ser1490), α-LRP6, α-β-catenin, α-E-cadherin, α-N-cadherin, α-Vimentin, α-Snail, α-Histone H3, α-c-Myc, α-cyclin D1, α-Axin, α-CD44, α-HIF-1a, α-Glut1 (Cell Signaling Technology, USA), α-VEGFA (Abcam, Cambridge, UK), α-β-actin.
4
Western Blot Analysis of EMT Markers
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The whole cell proteins of MDA-MB-231/MDA-MB-468 cells were extracted from cells using RIPA buffer (Beyotime, Shanghai, China) and the nuclear extracts were prepared with a NE-PER Nuclear and Cytoplasmic Extraction Kit (Pierce Biotechnology, Rockford, USA). Western blots were probed with α-phospho LRP6 (Ser1490), α-LRP6, α-β-catenin, α-E-cadherin, α-N-cadherin, α-Vimentin, α-Snail, α-Histone H3, α-c-Myc, αcyclin D1, α-Axin, α-CD44, α-HIF-1a, α-Glut1 (Cell Signaling Technology, USA), α-VEGFA (Abcam, Cambridge, UK), α-β-actin.
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