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Brdu labeling kit

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The BrdU labeling kit is a laboratory reagent used for the detection and quantification of DNA synthesis and cell proliferation. The kit provides the necessary components, including the BrdU (5-bromo-2'-deoxyuridine) labeling reagent, for performing these analyses.

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Lab products found in correlation

Bromodeoxyuridine (brdu), by BD (2 mentions) Lsm880 laser scanning confocal microscope, by Zeiss (1 mentions) Alexa fluor 594 conjugated goat anti mouse igg, by Thermo Fisher Scientific (1 mentions) Mouse anti brdu monoclonal antibody, by Merck Group (1 mentions) Facs caliber, by BD (1 mentions) Click it plus edu flow cytometry assay kit, by Thermo Fisher Scientific (1 mentions) Anti brdu antibody, by BD (1 mentions) Bromodeoxyuridine (brdu), by Merck Group (1 mentions) Fitc anti hla abc antibody clone w6 32, by BioLegend (1 mentions)

Close spelling variants of this product

BrdU-FITC labeling kit

9 protocols using brdu labeling kit

1

BrdU Labeling in Mice

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Mice were injected with 1 mg BrdU (BD Biosciences) i.p. every 12 h for 3 consecutive days. Mice were sacrificed 12 hours after the final injection and cells were stained using a BrdU labeling kit (BD Biosciences) following the manufacturer’s instructions.
Huynh A., DuPage M., Priyadharshini B., Sage P.T., Quiros J., Borges C.M., Townamchai N., Gerriets V.A., Rathmell J.C., Sharpe A.H., Bluestone J.A, & Turka L.A. (2015). The phosphatase PTEN-mediated control of PI-3 kinase in Tregs cells maintains homeostasis and lineage stability. Nature immunology, 16(2), 188-196.
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2

BrdU Labeling in Mice

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Mice were injected with 1 mg BrdU (BD Biosciences) i.p. every 12 h for 3 consecutive days. Mice were sacrificed 12 hours after the final injection and cells were stained using a BrdU labeling kit (BD Biosciences) following the manufacturer’s instructions.
Huynh A., DuPage M., Priyadharshini B., Sage P.T., Quiros J., Borges C.M., Townamchai N., Gerriets V.A., Rathmell J.C., Sharpe A.H., Bluestone J.A, & Turka L.A. (2015). The phosphatase PTEN-mediated control of PI-3 kinase in Tregs cells maintains homeostasis and lineage stability. Nature immunology, 16(2), 188-196.
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3

BrdU Immunolabeling of NSCLC Cells

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A549 and H460 human NSCLC cells were cultured in slide chambers and labeled with BrdU using a BrdU labeling kit (BD Biosciences) according to the manufacturer’s instructions. Slides were blocked with 5% normal goat serum for 30 min prior to incubation with a mouse anti-BrdU monoclonal antibody (1:200, Sigma) overnight at 4°C. After intensive washes, cells were incubated with Alexa Fluor-594 conjugated goat anti-mouse IgG (1:200, Invitrogen) for 1h at room temperature and nuclei were counterstained with DAPI. Finally, slides were imaged and analyzed using a Zeiss LSM 880 laser scanning confocal microscope (Carl Zeiss, Oberkochen Germany).
He X., Yang A., McDonald D.G., Riemer E.C., Vanek K.N., Schulte B.A, & Wang G.Y. (2017). MiR-34a modulates ionizing radiation-induced senescence in lung cancer cells. Oncotarget, 8(41), 69797-69807.
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4

BrdU-based Proliferation Assay in ARPE-19 Cells

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The cell proliferation rate was assessed as mentioned earlier using the BrdU labeling kit (BD Biosciences, San Diego CA, USA) in BEV and BEV + RES treated ARPE-19 cells7 (link). Briefly, after BEV and BEV + RES treatment, the cells were incubated with BrdU and then collected and stained as per the manufacturer’s instructions. Using flow cytometry, FACS caliber™ (BD Biosciences, San Diego, CA, USA) stained cells were acquired and analyzed in the FL1 channel using the BD CellQuest™ Pro software (BD FACSCaliber™, San Diego, CA, USA).
Subramani M., Ponnalagu M., Krishna L., Jeyabalan N., Chevour P., Sharma A., Jayadev C., Shetty R., Begum N., Archunan G, & Das D. (2017). Resveratrol reverses the adverse effects of bevacizumab on cultured ARPE-19 cells. Scientific Reports, 7, 12242.
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5

Bone Marrow Cell Proliferation Assay

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Cell proliferation was determined using a BrdU labeling kit (BD bioscience) or a Click-iT™ Plus EdU Flow Cytometry Assay Kits (Invitrogen). Twenty four hours before sacrifice, BrdU was administrated by intraperitoneal injection (2 mg/mouse in 200 µl PBS) as a single dose. At indicated time points, LSK, GMP, CMP, MEP cells were sorted from BM BMMC. Sorted cells were fixed in 70% ethanol overnight at –20°C, denatured in 2N HCl/0.5% Triton X-100 for 20 minutes at room temperature, neutralized with 0.1 M sodium borate for 5 minutes, and stained with anti-BrdU antibody (BD Biosciences, MD USA) for 30 minutes at room temperature in PBS/0.5% BSA/0.5% Tween 20. Cells were then resuspended in 500 µl PBS containing 10 µg RNase A and 5 µg Propidium Iodide (PI), incubated for 30 minutes, and immediately analyzed by flow cytometry. The mean frequencies of BrdU+ cells in the HSC and each progenitor populations were measured. For the EdU incorporation assay, EdU was administrated by intraperitoneal injection (0.5 mg/mouse in 200 µl PBS) as a single dose 24 hr before sacrificing the mice. The isolated cells were prepared and stained following a protocol provided by the manufacturer ((Invitrogen).
Zhu H., Kwak H.J., Liu P., Bajrami B., Xu Y., Park S.Y., Nombela-Arrieta C., Mondal S., Kambara H., Yu H., Chai L., Silberstein L.E., Cheng T, & Luo H.R. (2017). Reactive oxygen species-producing myeloid cells act as a bone marrow niche for sterile inflammation-induced reactive granulopoiesis. Journal of immunology (Baltimore, Md. : 1950), 198(7), 2854-2864.
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6

BrdU Labeling in Mice

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Mice were injected with 5-bromo-deoxyuridine (BrdU; 100 mg/kg i.p. BD Biosciences San Jose, CA) and then sacrificed 3 hours later. BrdU staining was performed using a BrdU labeling kit (BD).
Dai H., He F., Tsokos G.C, & Kyttaris V.C. (2017). IL-23 limits the production of IL-2 and promotes autoimmunity in lupus. Journal of immunology (Baltimore, Md. : 1950), 199(3), 903-910.
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7

In Vivo BrdU Labeling Assay

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The assay was performed as described earlier (Takizawa et al., 2011 (link)); briefly mice were i.p. injected with 180 μg BrdU (sigma) and were fed water containing 800 μg/ml BrdU and 4% glucose for 12 days. Mice were then sacrificed, organs (brain and spleen) were removed, and cells were isolated as described earlier. BrdU staining was performed using BrdU labeling kit (BD). Cells from normal water-fed mice were used as staining controls.
Boddupalli C.S., Nair S., Belinsky G., Gans J., Teeple E., Nguyen T.H., Mehta S., Guo L., Kramer M.L., Ruan J., Wang H., Davison M., Kumar D., Vidyadhara D., Zhang B., Klinger K, & Mistry P.K. (2022). Neuroinflammation in neuronopathic Gaucher disease: Role of microglia and NK cells, biomarkers, and response to substrate reduction therapy. eLife, 11, e79830.
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8

Cell Proliferation in Hematopoietic Stem and Progenitor Cells

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Cell proliferation was determined using a BrdU labeling kit (BD bioscience). Twenty four hours before sacrifice, BrdU was administrated by intraperitoneal injection (2 µg/mouse in 200 µl PBS) as a single dose. At indicated time points, LSK, GMP, CMP, MEP cells were sorted and the mean frequencies of BrdU+ cells in the HSC and each progenitor populations were measured.
Kwak H.J., Liu P., Bajrami B., Xu Y., Park S.Y., Nombela-Arrieta C., Mondal S., Sun Y., Zhu H., Chai L., Silberstein L.E., Cheng T, & Luo H.R. (2015). Myeloid cell-derived reactive oxygen species externally regulate the proliferation of myeloid progenitors in emergency granulopoiesis. Immunity, 42(1), 159-171.
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9

BrdU Labeling and Flow Cytometry

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Cells were cultured in the presence of 10 μM BrdU for 30 minutes, and then BrdU was detected following the manufacturer’s protocol (BrdU Labeling kit, Cat #: 552598, BD Biosciences, San Jose, CA). Cells were also stained with a FITC-anti-HLA-ABC antibody (clone W6/32: Cat #: 311404, BioLegend, San Diego, CA). PCa cells were gated as HLA-positive populations and BrdU-positive cells were detected by a FACS Aria dual-laser flow cytometer.
Yumoto K., Eber M.R., Wang J., Cackowski F.C., Decker A.M., Lee E., Nobre A.R., Aguirre-Ghiso J.A., Jung Y, & Taichman R.S. (2016). Axl is required for TGF-β2-induced dormancy of prostate cancer cells in the bone marrow. Scientific Reports, 6, 36520.
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