Anti nucleophosmin

Equal amounts of protein from each sample were separated by 4–20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to nitrocellulose membranes, and incubated with the following primary antibodies: anti-axin2 (Cell Signaling, #2151), anti-β-catenin (Cell Signaling, #8480), anti-lactate dehydrogenase A (Cell Signaling, #2012), anti- phosphoglycerate kinase 1 (Santa Cruz, sc-130335), anti-glyceraldehyde 3-phosphate dehydrogenase (Santa Cruz, sc-25778), anti-nucleophosmin (Abcam, ab10530), anti-nucleolin (Cell Signaling, 14574), anti-drebrin 1 (Abnova, H00001627-M03), anti-glycogen synthase kinase 3 α/β (Cell Signaling, #5676), and anti-gelsolin (Cell Signaling, #12953). Anti-β-actin (Sigma-Aldrich, A5441) was used as a loading control. After washing, the blots were labeled with horseradish peroxidase-conjugated secondary antibodies and visualized with the ECL reagent (GE Healthcare) according to the manufacturer’s protocol.