Serum and brain tissue samples were run on multiplex arrays for a range of targets using one of two arrays: Bio-Plex Pro Mouse Cytokine 23-Plex Immunoassay (Bio-Rad, Hercules, California, USA), which tested 23 targets in duplicate, or Quantibody® Mouse Inflammation Array 1 Kit (RayBiotech, Peachtree Corners, GA, USA), which tested 40 targets in quadruplicate. The lists of target molecules for the two arrays substantially overlap but are not identical, and all targets are displayed in the figures and supplementary material. Validation of multiplex experiments were performed using ELISA with most samples run in duplicate except where otherwise indicated. Mouse Quantikine ELISA kits (R&D Systems, Minneapolis, Minnesota, USA) were used to measure eotaxin (MME00), IL-5 (M5000), and IL-6 (M6000B) following the manufacturer's instructions.
Quantibody mouse inflammation array 1 kit
Manufactured by RayBiotech
Sourced in United States
The Quantibody® Mouse Inflammation Array 1 Kit is a multiplex ELISA-based assay designed to simultaneously detect and quantify the levels of 40 different mouse inflammatory proteins in a single sample. The kit utilizes an array-based platform to provide a comprehensive analysis of the inflammatory profile in a variety of mouse biological samples.
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Lab products found in correlation
3 protocols using quantibody mouse inflammation array 1 kit
1
Multiplex Cytokine Profiling in Murine Samples
2
Cytokine Release Assay in CT2A-hEGFR Mice
3
Bleomycin-Induced Lung Inflammation Markers
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Expression of inflammatory markers in lung tissue lysates from animals exposed to bleomycin (including those also treated with ASTEX, DF-EVs, or vehicle) or sham control (day 8 postexposure) were measured using a Quantibody® Mouse Inflammation Array 1 Kit (RayBiotech, Peachtree Corners, GA, USA) per manufacturer’s instructions.
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