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3 protocols using tridodecylmethylammonium chloride

1

Boronic Acid-Functionalized Nanoparticles

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Carboxylatedpoly(vinyl chloride) (>97% GC) (PVCCOOH), bis-(2-ethylhexyl)sebacate (DOS), polycaprolactone (Mn 70,000-90,000) (PCL), tridodecylmethylammonium chloride (TDMAC), alizarin, 4-carboxy-3-fluorophenylboronic acid (1), 3-fluoro-4-methoxycarbonylphenylboronic acid (2a), D-(+)-glucose, tetrahydrofuran (≥ 99.9%) (THF), dicyclohexylcarbodiimide solution (60% w/v in xylene) (DCC), N-hydroxysuccinimide (NHS), aniline(≥ 99.5%), 1-propanol (anhydrous, 99.7%), 1-butanol (HPLC, 99.7%), 1-hexanol (98%), cyclohexanol(99%),sodium sulfate (anhydrous,≥ 99.9%), sodium chloride, ethyl acetate (anhydrous, 99.8%), hexane (anhydrous, 99.5%), N, N’-dimethylformamide (DMF) and N, N’-dimethylaminopyridine (DMAP) were purchased from Sigma Aldrich (St Louis, MO, USA). Octylboronic acid (>97%) and Citroflex A-6 were acquired from Synthonix (Wake Forest, NC, USA) and Vertellus (Indianapolis, IN, USA), respectively. Phosphate Buffered Saline (PBS) (1x, pH = 7.4) was purchased as a solution from Invitrogen (Carlsbad, CA, USA). Hydrochloric acid (1.0 N) and sodium bicarbonate were purchased from Fisher Scientific (Fair Lawn, NJ, USA). 1,2-distearoyl-snglycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-550] (ammonium salt) (DSPE-mPEG550) was purchased from Avanti Polar Lipids, Inc. SKH1-E mice were acquired from Charles River Laboratories International Inc. (Wilmington, MA).
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2

Fluorescent Polymer Nanomaterial Synthesis

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Poly (vinyl chloride), high molecular weight (PVC), bis(2-ethylhexyl) sebacate (DOS), chromoionophore IV (CHIV), chloride ionophore IV (Cl IV), Tridodecyl methyl ammonium chloride (TTDMAC), tetrahydrofuran (THF), 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES), dichloromethane (DCM), sodium chloride, Pluronic F127 was purchased from Sigma-Aldrich (St. Louis, MO, USA). 1,2-Disteroyl-sn-glycero-3-phosphoethanolamine-N- [methoxy (polyethylene glycol)-550] ammonium salt in chloroform (DSPE-PEG) was purchased from Avanti Polar Lipids (Alabaster, AL, USA). (Dil) was purchased from Life Technologies (Grand Island, NY, USA). CellBrite™ NIR750 was purchased from Biotium (Fremont, CA). 2-Amino-2-hydroxymethylpropane-1,3-diol, 2 M solution (TRIS, 2M), was purchased from Fisher Scientific (Waltham, MA, USA). Phosphate-buffered saline (PBS with Ca2+ and Mg2+, pH = 7.4) was purchased from Boston Bioproducts (Ashland, MA, USA).
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3

Synthesis of Ion-Selective Membranes

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All aqueous solutions were prepared using deionized and charcoal-treated water (>18.2 MΩ cm specific resistance) obtained with a Milli-Q PLUS reagent grade water system (Millipore, Bedford, MA). High molecular weight poly(vinyl chloride), 2-nitrophenyl octyl ether, tridodecylmethylammonium chloride, sodium 1-dodecyl sulfate (≥ 99%, by titration with benzethonium chloride), 1-octyl sulfate, and tetrahydrofuran (anhydrous; inhibitor-free) were purchased from Sigma-Aldrich (St. Louis, MO), poly(dimethylamine-co-epichlorohydrin) (MW = 240000; repeat unit MW = 137.6) from Accepta (Manchester, UK), and NaCl and Tygon S3 tubing (4.8 mm inner diameter; 7.9 mm outer diameter) from Fisher Scientific (Waltham, MA). All materials were used as received.
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