M200 pro multimode microplate reader

All analytical grade reagents were purchased from Sigma-Aldrich (Numbrecht, Germany/Saint Louis, MO, USA). First, all solutions were sterilized by filtration through 0.2 mm membrane filters directly before use.
To differentiate the results obtained for agomelatine, aminoguanidine was used, as a known protein oxidation inhibitor, and α-lipoic acid (ALA), as an antioxidant. The concentration of all additives was 1 mM (based on in vitro, kinetic studies), in proportion to the high concentrations of the glycating agents (21 (link)–26 (link)).
The absorbance and fluorescence were assessed with an M200 PRO multimode microplate reader (Tecan Group Ltd., Männedorf, Switzerland).

All reagents (analytical grade) were purchased from Sigma-Aldrich (Nümbrecht, Germany, or Saint Louis, MO, USA). Solutions were sterilized by filtration through 0.2 mm membrane filters directly before use. The fluorescence and absorbance were evaluated using a microplate reader (M200 PRO multimode microplate reader; Tecan Group Ltd., Männedorf, Switzerland).

Quantification of the antimicrobial resistance against various AMPs was carried out by measuring the bacterial growth in the presence of peptides at serial dilutes in 96 well plates in a microplate (96-well plate) reader Infinite^® M200 PRO Multimode Microplate Reader (Tecan, Maennedorf, Switzerland) according to our recent reports [67 (link)]. All bacterial strains were incubated at 32 °C for 13–18 h at 200 rpm. Optical density measurements (λ = 550 nm) were taken automatically at 10 min intervals. Antimicrobial resistance activity was quantified as the minimum inhibitory concentration (MIC)—the lowest concentration of a peptide which prevents growth of bacterial cells.