Log In Sign up
The largest database of trusted experimental protocols

Standard rat operant chambers

Manufactured by Med Associates
Visit Supplier
Sourced in United States

Standard rat operant chambers are laboratory equipment used to study animal behavior. The chambers provide a controlled environment for experiments involving rodents, such as rats. The core function of these chambers is to allow researchers to monitor and record the animals' responses to various stimuli or tasks.

Automatically generated - may contain errors

Lab products found in correlation

45 mg pellet, by Bio-Serv (2 mentions) F06162, by Bio-Serv (2 mentions) Infusion pump, by Med Associates (1 mentions) Tygon tubing, by Cole-Parmer (1 mentions)

12 protocols using standard rat operant chambers

1

Operant Conditioning Rat Study

Check if the same lab product or an alternative is used in the 5 most similar protocols
The apparatus consisted of 16 standard rat operant chambers (Med Associates, St. Albans, VT), housed in sound attenuating cubicles. All chambers were equipped with two levers situated on either side of a pellet receptacle: the left lever was the timing lever for all rats. Rats received 45-mg pellets (Bio-Serv, Flemington, NJ) as rewards. A 28-V 100-mA house light was used as a to-be-timed stimulus. An 80-dB white noise stimulus was used as a distracter stimulus for all rats.
Matthews A.R., Buhusi M, & Buhusi C.V. (2020). Blockade of Catecholamine Reuptake in the Prelimbic Cortex Decreases Top-down Attentional Control in Response to Novel, but Not Familiar Appetitive Distracters, within a Timing Paradigm. NeuroSci, 1(2), 99-114.
+ Open protocol
+ Expand
2

Rat Operant Conditioning for High-Fat Food

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Between 13:00 and 17:00, rats were placed in standard rat operant chambers (Med Associates, Georgia, VT). Responding on the lever associated with food delivery resulted in delivery of a high-fat food pellet (45% energy from fat, 45mg, Bioserv F06162, Frenchtown, NJ). Rats were trained in half-hour sessions on a fixed ratio (FR) 1 schedule, where a single response on the active lever is needed to receive a pellet.
Once the percentage of responses on the active lever exceeded 85% for three consecutive days (30 (link)), animals were advanced to an FR3 and then an FR5 schedule, which require 3 and 5 correct responses for pellet delivery, respectively. Once animals have reached this criterion on the FR5, they move to a progressive ratio (PR) schedule, where earning each successive high-fat food pellet within the session requires a greater number of responses (1,2,3,6,9,12,15,20,25,32,40,50,62,77,95). Responses on the active, reinforced lever within a 60-minute test session were quantified. On test day, animals (n=11/group) received an IP injection of 0.3mg/kg NMU in DMSO (6 (link)), or vehicle alone fifteen minutes prior to testing (total volume 3mL).
McCue D.L., Kasper J.M, & Hommel J.D. (2016). Regulation of Motivation for Food by Neuromedin U in the Paraventricular Nucleus and the Dorsal Raphe Nucleus. International journal of obesity (2005), 41(1), 120-128.
+ Open protocol
+ Expand
3

Operant Conditioning Rat Study

Check if the same lab product or an alternative is used in the 5 most similar protocols
The apparatus consisted of 16 standard rat operant chambers (Med Associates, St. Albans, VT), housed in sound attenuating cubicles. All chambers were equipped with two levers situated on either side of a pellet receptacle: the left lever was the timing lever for all rats. Rats received 45-mg pellets (Bio-Serv, Flemington, NJ) as rewards. A 28-V 100-mA house light was used as a to-be-timed stimulus. An 80-dB white noise stimulus was used as a distracter stimulus for all rats.
Matthews A.R., Buhusi M, & Buhusi C.V. (2020). Blockade of Catecholamine Reuptake in the Prelimbic Cortex Decreases Top-down Attentional Control in Response to Novel, but Not Familiar Appetitive Distracters, within a Timing Paradigm. NeuroSci, 1(2), 99-114.
+ Open protocol
+ Expand
4

Operant Conditioning in Rat Chambers

Check if the same lab product or an alternative is used in the 5 most similar protocols
All training and testing was done in standard rat operant chambers (Med Associates Inc.). A houselight provided constant, low-level illumination. Recessed sucrose delivery ports with infrared beams that detected head entry were located on the left and right sides of the chamber. Two lights, used as conditioned and discriminative stimuli were located a few centimeters above the ports, one light above each port. One retractable lever was located between the ports. Sucrose solution was delivered to a well in each port via a programmable pump.
Shabel S.J., Murphy R.T, & Malinow R. (2014). Negative learning bias is associated with risk aversion in a genetic animal model of depression. Frontiers in Human Neuroscience, 8, 1.
+ Open protocol
+ Expand
5

Cocaine Self-Administration and Go-NoGo Task

Check if the same lab product or an alternative is used in the 5 most similar protocols
Operant training took place as described in detail previously (Zapata et al., 2017 (link)). Standard rat operant chambers (Med-Associates; St Albans, VT) were used. Rats were trained to self-administered cocaine (0.75 mg/kg /infusion) for 12 sessions (2 hours or 40 infusions per session) on an FR1 schedule. Following this, they were trained on a Go-NoGo task consisting of 2 hour sessions comprised of 6 × 20 min alternating intervals of cocaine availability (Go) and non–availability (NoGo), signaled by the house light (light on during cocaine availability). During Go intervals, responses were reinforced with cocaine under an FR5 schedule, and during NoGo intervals lever responses did not trigger cocaine infusions. Training progressed until stable discrimination of the Go/NoGo periods were observed (3 consecutive sessions in which NoGo responses were less than 30% of total, 12–14 sessions). After reaching criterion, testing proceeded using a within subject design in which each rat received bilateral infusion of PBS or AM251 (0.7 ug) before intraperitoneal (i.p.) injections of either Δ9-THC or vehicle, in a randomized order, 15 min before each test session.
Zapata A, & Lupica C.R. (2021). Lateral habenula cannabinoid CB1 receptor involvement in drug-associated impulsive behavior. Neuropharmacology, 192, 108604.
+ Open protocol
+ Expand
6

Operant Conditioning in Rats

Check if the same lab product or an alternative is used in the 5 most similar protocols
Behavioural testing took place within standard rat operant chambers (Med‐Associates, Vermont, USA). Chambers were housed within sound and light attenuating boxes. Each chamber consisted of a house light and two retractable levers either side of food magazine. Standard food pellets (45 mg; BioServ) were delivered to the magazine via an automated dispenser. Experimental sessions were governed by programmes written with Med‐PC software.
Hailwood J.M., Gilmour G., Robbins T.W., Saksida L.M., Bussey T.J., Marston H.M, & Gastambide F. (2018). Oxygen responses within the nucleus accumbens are associated with individual differences in effort exertion in rats. The European Journal of Neuroscience, 48(9), 2971-2987.
+ Open protocol
+ Expand
7

Dual Heroin and Food Self-Administration in Rats

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Rats were surgically implanted with an intravenous jugular catheter as previously described [6 (link)]. After recovery, rats were trained to self-administer food and heroin in tandem over daily (weekday) sessions inside standard rat operant chambers (Med Associates, St. Albans, VT, USA). During the first 30 min of each session, the food (right) lever and cue (3.5 kHz tone, 5 sec) were available, followed by a 10 min time-out with levers retracted. Thereafter, the heroin (left) lever and cue (light above lever, 5 sec) were available for 2 h. Training began on a fixed-ratio 1 (FR1) schedule of reinforcement. Cues for each reward were initiated simultaneously with reward delivery onset, and levers retracted during cue presentation. After 8 sessions, training progressed through 8 additional FR steps (FR3, FR8, FR20, FR50, FR125, FR313, FR783, FR1958) with at least 1 day on each FR. To prevent infection and catheter occlusion, respectively, cefazolin and taurolidine citrate solution (TCS) were administered after each self-administration session. Catheter patency was periodically verified using methohexital sodium (1 mg/0.1 ml, i.v.). Five rats were excluded from the final dataset due to defective catheters.
Giannotti G., Mottarlini F., Heinsbroek J.A., Mandel M.R., James M.H, & Peters J. (2022). Oxytocin and orexin systems bidirectionally regulate the ability of opioid cues to bias reward seeking. Translational Psychiatry, 12, 432.
+ Open protocol
+ Expand
8

Rat Operant Conditioning for High-Fat Food

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Between 13:00 and 17:00, rats were placed in standard rat operant chambers (Med Associates, Georgia, VT). Responding on the lever associated with food delivery resulted in delivery of a high-fat food pellet (45% energy from fat, 45mg, Bioserv F06162, Frenchtown, NJ). Rats were trained in half-hour sessions on a fixed ratio (FR) 1 schedule, where a single response on the active lever is needed to receive a pellet.
Once the percentage of responses on the active lever exceeded 85% for three consecutive days (30 (link)), animals were advanced to an FR3 and then an FR5 schedule, which require 3 and 5 correct responses for pellet delivery, respectively. Once animals have reached this criterion on the FR5, they move to a progressive ratio (PR) schedule, where earning each successive high-fat food pellet within the session requires a greater number of responses (1,2,3,6,9,12,15,20,25,32,40,50,62,77,95). Responses on the active, reinforced lever within a 60-minute test session were quantified. On test day, animals (n=11/group) received an IP injection of 0.3mg/kg NMU in DMSO (6 (link)), or vehicle alone fifteen minutes prior to testing (total volume 3mL).
McCue D.L., Kasper J.M, & Hommel J.D. (2016). Regulation of Motivation for Food by Neuromedin U in the Paraventricular Nucleus and the Dorsal Raphe Nucleus. International journal of obesity (2005), 41(1), 120-128.
+ Open protocol
+ Expand
9

Operant Chambers for Fentanyl Self-Administration

Check if the same lab product or an alternative is used in the 5 most similar protocols
Standard rat operant chambers (Med Associates, St Albans, VT, USA) were used for IV fentanyl self-administration. Each operant chamber had plexiglass front and back walls, metal side walls, a metal grid floor, and a removable tray below the grid floor filled with bedding. The chambers contained two retractable levers with a cue light located above each lever. Fentanyl was delivered through Tygon tubing (Cole-Parmer, Vernon Hills, IL, USA) that connected to the rat’s catheter and a 30 ml BD luer lock syringe (Mckesson, USA) attached to an infusion pump (Med Associates) outside of the operant chamber. The tubing was protected by a modified leash (Med Associates) and held in place by a drug delivery arm (Med Associates) and swivel (Med Associates), which allowed the animals to move freely in the chambers. For all operant sessions, we provided animals with hard plastic chew toys (Bio-Serv Nylon Bones, Fisher Scientific) to minimize destructive chewing or self-injury.
Landry M.L, & Ferguson D. (2000). SimulFluor Respiratory Screen for Rapid Detection of Multiple Respiratory Viruses in Clinical Specimens by Immunofluorescence Staining. Journal of Clinical Microbiology, 38(2), 708-711.
+ Open protocol
+ Expand
10

Operant Conditioning in Rats: Standard Apparatus

Check if the same lab product or an alternative is used in the 5 most similar protocols
The apparatus consisted of 12 standard rat operant chambers (MED Associates, St. Albans, VT, USA) housed in sound attenuating cubicles. Chambers were equipped with two fixed levers situated on the front wall of the chamber. According to the schedule, 45 mg precision food pellets (BioServ, Frenchtown, NJ, USA) were delivered in a food cup situated on the front wall, 1 cm above the grid floor, between the two levers, by a pellet dispenser. The to-be-timed visual stimuli were two cue lights located about 7 cm above the levers. A 66-dB background sound produced by a ventilation fan was present throughout the session.
Buhusi C.V., Reyes M.B., Gathers C.A., Oprisan S.A, & Buhusi M. (2018). Inactivation of the Medial-Prefrontal Cortex Impairs Interval Timing Precision, but Not Timing Accuracy or Scalar Timing in a Peak-Interval Procedure in Rats. Frontiers in Integrative Neuroscience, 12, 20.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!