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Goat anti vimentin polyclonal antibody

Manufactured by Santa Cruz Biotechnology

Goat-Anti-Vimentin polyclonal antibody is a primary antibody produced in goats and used to detect the vimentin protein. Vimentin is a type III intermediate filament protein found in various non-epithelial cells.

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2 protocols using goat anti vimentin polyclonal antibody

1

Immunohistochemical Staining Protocol

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Detailed procedures for IHC staining have been described previously39 (link). Signals were visualized using SuperPicTure™ Polymer Detection kits (Life Technologies). The antibodies used were: Rabbit-Anti-Cytokeratin 7 polyclonal antibody (Novus); Rat-Anti-Cytokeratin 8 monoclonal antibody (DSHB); Goat-Anti-Vimentin polyclonal antibody (Santa Cruz).
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2

Western Blot Protein Analysis

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Cell and tissue lysates preparation and Western blotting were performed as described previously39 (link). Detailed procedure can be found in Supplementary Materials and Methods. The antibodies used were: Mouse-Anti-GNMT monoclonal antibody40 (link); Goat-Anti-vimentin polyclonal antibody (Santa Cruz); Rabbit-Anti-N-cadherin polyclonal antibody (Abcam); Rat-Anti-E-cadherin monoclonal antibody (Abcam); Mouse-Anti-GAPDH (Millipore).
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