Fitc anti s100

Manufactured by Abcam

FITC-Anti-S100 is a fluorescently labeled antibody that specifically binds to the S100 protein. S100 is a calcium-binding protein found in a variety of cell types. The FITC (Fluorescein Isothiocyanate) label allows for the detection and visualization of S100-expressing cells and tissues using fluorescence-based techniques.

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Anti-S100 (18 citations)

2 protocols using fitc anti s100

Multi-Parametric Flow Cytometry and Molecular Analyses

Cited 2 times

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For multi-parametric flow cytometry, immunofluorescence/immunohistochemistry and DEPArray analyses, primary antibodies were obtained from the following sources: FITC-CD45 (#304054; 1:200), FITC-CD34 (#343504; 1:200), FITC-CD105 (#323204; 1:200), FITC-CD90 (#328108; 1:200), FITC-CD73 (#344016; 1:200), FITC HLA-A/B/C antibody (#311404; 1:200), PerCP/Cy5.5-CD146 (#342014; 1:100), PE-Human NG2/MCSP (#FAB2585P, 1:100), BV421-Ki67 (#350506; 1:100), were obtained from Biolegend, Inc. APC-Cy7-CD44 (#103028, 1:100) BV510-CD24 (#311126, 1:100), PE-Pan-Cytokeratin (#5075, 1:100) were purchased from Cell Signaling Technology, Inc. Anti-Mel-A antibody (# AC12-0297-03; 1:200) was obtained from Abcore, and FITC-Anti-S100 (#ab76749; 1:50) was purchased from Abcam. For immunohistochemistry, anti-human, anti-Mel-A antibody (# ab51061; 1:100), HLA-ABC (#565292; 1:100) were obtained from BD Biosciences, Inc. (San Jose, CA, USA). Anti-mouse secondary IgG anti-human antibodies used for IHC staining were received from Santa Cruz Biotechnology, Inc. Antibodies for immunofluorescence staining were obtained from Cell Signaling Technology, Inc. (1:500 dilution of stock solution), as previously described [3 (link),22 (link)]. NRF2 inhibitor ML385 (#2114) was obtained from Cayman, Inc. while Nodal inhibitor Lefty (746-LF/CF) was purchased from R&D Systems, Inc. (Waltham, MA, USA).

Sprouse M.L., Welte T., Boral D., Liu H.N., Yin W., Vishnoi M., Goswami-Sewell D., Li L., Pei G., Jia P., Glitza-Oliva I.C, & Marchetti D. (2019). PMN-MDSCs Enhance CTC Metastatic Properties through Reciprocal Interactions via ROS/Notch/Nodal Signaling. International Journal of Molecular Sciences, 20(8), 1916.

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Multiparametric Flow Cytometry and Immunohistochemistry

Check if the same lab product or an alternative is used in the 5 most similar protocols

For multi-parametric flow cytometry and DEPArray, primary antibodies were obtained from the following sources: FITC-CD45 (#304054; 1:200), FITC-CD34 (#343504; 1:200), FITC-CD105 (#323204; 1:200), FITC-CD90 (#328108; 1:200), FITC-CD73 (#344016; 1:200), FITC HLA-A/B/C antibody (#311404; 1:200), PerCP/Cy5.5-CD146 (#342014; 1:100), PE-Human NG2/MCSP (#FAB2585P, 1:100), BV421-Ki67 (#350506; 1:100), were obtained from Biolegend, anti-Melan-A antibody (# AC12-0297-03; 1:200) from Abcore, and FITC-Anti-S100 (#ab76749; 1:50) was purchased from Abcam.
For immunohistochemistry, anti-human, anti-Melan-A antibody (# ab51061; 1:100), anti-tenascin-C (# ab108930, 1:100) and anti-p21 (#ab188224, 1:100) were purchased from Abcam, HLA-ABC (#565292; 1:100) from BD Biosciences, USP7 (#GTX125894; 1:200) from Genetex, and PTEN antibody (# sc-7974; 1:20) was purchased from Santa Cruz Biotechnology, anti-p53 antibody (#SAB4503021, 1:100) was purchased from Sigma, CCP110 (#12780-I-AP, 1:100) antibody was obtained from Proteintech. Anti-mouse, USP7 (#26948-1-AP, 1:200) and PTEN (#603000-1-Ig, 1:200) antibodies were purchased from Proteintech, Alexafluor-conjugated anti-mouse, anti-rabbit secondary IgG antibodies used for immunofluorescence staining (1:500 dilution) were obtained from Cell Signaling Technology. USP7 inhibitors P5091 (#SML0770) and P22077 (#2301) were purchased from Biovision.

Vishnoi M., Boral D., Liu H., Sprouse M.L., Yin W., Goswami-Sewell D., Tetzlaff M.T., Davies M.A., Glitza Oliva I.C, & Marchetti D. (2018). Targeting USP7 identifies a metastasis-competent state within bone marrow-resident melanoma CTCs. Cancer research, 78(18), 5349-5362.

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