Log In Sign up
The largest database of trusted experimental protocols

Cy3 labeled telomere peptide nucleic acid probe

Manufactured by Panagene
Visit Supplier

The Cy3-labeled telomere peptide nucleic acid probe is a laboratory equipment used for the detection and analysis of telomere regions in DNA samples. It consists of a peptide nucleic acid (PNA) molecule labeled with the Cy3 fluorescent dye. The core function of this probe is to hybridize with complementary telomeric DNA sequences, enabling the visualization and quantification of telomere lengths in various experimental applications.

Automatically generated - may contain errors

Lab products found in correlation

Colcemid, by Merck Group (3 mentions)

3 protocols using cy3 labeled telomere peptide nucleic acid probe

1

Metaphase Chromosomes Telomere Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
For analysis of metaphase chromosomes, activated B cells were arrested with 100 ng/mL colcemid (Sigma) for 1 hr prior to collection at each time point. This was followed by treatment with hypotonic solution (0.075 M KCl) and fixation with 3:1 methanol/acetic acid. Telomere-FISH analysis was performed with Cy3-labeled telomere peptide nucleic acid probe (Panagene). 50–55 images were analyzed per sample.
Simhadri S., Vincelli G., Huo Y., Misenko S., Foo T.K., Ahlskog J., Sorensen C.S., Oakley G.G., Ganesan S., Bunting S.F, & Xia B. (2018). PALB2 connects BRCA1 and BRCA2 in the G2/M checkpoint response. Oncogene, 38(10), 1585-1596.
+ Open protocol
+ Expand
2

Telomere-FISH Analysis of Activated B Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Chromosomal aberrations of activated B cells were determined as previously described (24 (link)). Briefly, cells were arrested with 100 ng/mL colcemid (Sigma) for 1 h prior to collection at each time point. This was followed by treatment with hypotonic solution (0.075 M KCl) and fixation with 3:1 methanol/acetic acid. Telomere-FISH analysis was performed with Cy3-labeled telomere peptide nucleic acid probe (Panagene). 50–55 images were analyzed per sample.
Foo T.K., Vincelli G., Huselid E., Her J., Zheng H., Simhadri S., Wang M., Huo Y., Li T., Yu X., Li H., Zhao W., Bunting S.F, & Xia B. (2021). ATR/ATM-mediated phosphorylation of BRCA1 T1394 promotes homologous recombinational repair and G2/M checkpoint maintenance. Cancer research, 81(18), 4676-4684.
+ Open protocol
+ Expand
3

Metaphase Chromosomes Telomere Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
For analysis of metaphase chromosomes, activated B cells were arrested with 100 ng/mL colcemid (Sigma) for 1 hr prior to collection at each time point. This was followed by treatment with hypotonic solution (0.075 M KCl) and fixation with 3:1 methanol/acetic acid. Telomere-FISH analysis was performed with Cy3-labeled telomere peptide nucleic acid probe (Panagene). 50–55 images were analyzed per sample.
Simhadri S., Vincelli G., Huo Y., Misenko S., Foo T.K., Ahlskog J., Sorensen C.S., Oakley G.G., Ganesan S., Bunting S.F, & Xia B. (2018). PALB2 connects BRCA1 and BRCA2 in the G2/M checkpoint response. Oncogene, 38(10), 1585-1596.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!