1 fluoro 2 4 dinitrofluorobenzene

Chemicals and reagents. The reagents, 1-fluoro-2,4-dinitrofluorobenzene (dinitrofluorobenzene; DNFB), dimethyl sulfoxide (DMSO), dexamethasone (DEX), phorbol 12-myristate 13-acetate (PMA), calcium ionophore A23187, nuclear fast red solution and fetal bovine serum (FBS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Alcian blue was purchased from Muto Pure Chemical (Tokyo, Japan). Dulbecco's Modified Eagle's Medium (DMEM/high glucose), penicillin and streptomycin were purchased from GE Healthcare (Logan, UT, USA). PrestoBlue cell viability reagent was purchased from Invitrogen Life Technologies (Carlsbad, CA, USA). The Cytometric Bead Array kit was purchased from BD Biosciences (Franklin Lakes, NJ, USA). The histamine assay kit, including -nitrophenyl-N-acetyl-β -D-glucosaminide was purchased from Oxford Biomedical Research (Rochester Hills, MI, USA).