Ftir spectrum 2
The PerkinElmer Spectrum-2 FTIR (Fourier Transform Infrared) spectrometer is a laboratory instrument used for the analysis of molecular structures. It measures the absorption of infrared radiation by a sample, generating a spectrum that can be used to identify and characterize various chemical compounds.
Lab products found in correlation
10 protocols using ftir spectrum 2
Nanoliposome Characterization and Analysis
FTIR Analysis of Hydrogel Spectra
Analytical Characterization of Compounds
Optical Properties of Graphene Quantum Dots
Characterization of Biofilm Matrices
FTIR Analysis of Metabolite Preparations
FTIR Analysis of Powder Samples
Characterization of Materials via Spectroscopic Techniques
spectra were recorded on a Perkin Elmer Spectrum 2 FT-IR instrument
(optical resolution of 0.5 cm–1) in attenuated total
reflectance (ATR) measurement mode (analyses in the 400–4000
cm–1 range, performed by averaging four acquisitions
per sample).
pXRD analyses were performed on an X’Pert
MPD diffractometer using Cu Kα1 radiation (λ
= 1.5406 Å) with the operating voltage and current maintained
at 40 kV and 25 mA, respectively. Diffractograms were recorded between
5 and 60° in 2θ, with a step size of 0.017° (with
a count time per step of ∼50 s).
SEM analyses were carried
out on a Zeiss Evo HD15 scanning electron
microscope equipped with an Oxford Instruments X-MaxN SDD 50 mm2 EDXS detector. Before the SEM analyses, samples were deposited
on a double-sided conducting carbon tape and then metallized with
carbon.
RITC-RGD:PB Particle Conjugation
volumetric ratio in 4 mM borate buffer as previously reported with
a similar method.68 (link) The solution was left
to mix overnight at RT. The following day, the particle mixture was
washed at 12 000 rpm for 30 min (×2) with DDI water. Final
RITC-RGD:PB particles are stored in water. Conjugation was validated
using a PerkinElmer Spectrum 2 FTIR through a drop cast method.
PEGylated Prussian Blue Nanoparticle Synthesis
Prussian Blue nanoparticles (PEG:PB) were prepared via a two-step
synthesis with some modifications.67 (link) Initially,
PBNPs were synthesized to include a PVP coating using a coprecipitation
reaction. Iron(III) chloride (1 mM) is mixed with potassium hexacyanoferrate
(II) trihydrate (1 mM) while in the presence of PVP and stirred at
60 °C overnight. The next day, the reaction mixture was washed
with a 1:1 water and acetone mixture at 12,000 rpm for 20 min (×3).
Particles were then PEGylated by doing a surfactant substitution.
PVP–PBNP (2 mg/mL) was mixed with an equal amount of poly bis(amine)
and stirred at RT for 24 h. The next day, the PEG:PB particles were
washed with DDI water (×3). Final solution was suspended in water.
Conjugation was validated using a PerkinElmer Spectrum 2 FTIR through
a drop cast method.
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