Qam cyt 1 1

Serum was collected from WT (n = 3) and Gzmb-HBZ (n = 3) mice after 16 hours without food and was frozen at -80 degrees Celsius. Thawed serum was applied to a glass slide antibody array and processed according to manufacturer’s instructions (QAM-CYT-1-1, Raybiotech). Raybiotech completed slide scanning and data extraction. The primary author analyzed raw data. For analysis, background was subtracted and mean values were determined (3-4 replicates per cytokine) and converted from fluorescence unit to protein concentration by a standard curve best-fit regression specific for each cytokine.

After the mice were subjected to 5% DSS for 5 days, 1 cm of the mouse proximal end of colon was added to 0.5 mL of tissue lysate (RIPA lysate and protease inhibitor; Applygen Technology Inc., Beijing, China). After homogenization and centrifugation at 12,000 × g, the supernatant was collected, and the total protein concentration was measured by BCA (Applygen Technology Inc., Beijing, China). Myeloperoxidase (MPO) levels were detected using an MPO ELISA kit (RayBiotech, Guangzhou, China), and cytokine levels were detected using a protein chip (QAM-CYT-1-1, RayBiotech, Guangzhou, China).