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86 protocols using zinc nitrate

1

Biogenic Synthesis of ZnO Nanocrystals

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Mature Prunus ϫ yedoensis leaves (50 g) were collected in and around Chonbuk National University, Iksan, South Korea and washed thoroughly with distilled water, cut into small pieces, and boiled in 200 ml of sterile Nanopure water (conductivity ϭ 18 μ Ω /m, TOC Ͻ 3 ppb, Barnstead, Waltham, MA, USA) for 30 min to obtain the extract, followed by fi ltration (Whatman No. 42) and storage in a refrigerator for future use. One hundred milliliters of 0.1 M zinc nitrate [(Zn(NO 3 ) 2 .6H 2 O)-, Sigma-Aldrich, St. Louis, MO, USA] was added dropwise using a burette into 20 ml of the hot leaf extract under continuous stirring at 80 ° C for 8 h. Th e ZnO nanocrystals were synthesized through reduction of zinc nitrate by the phytochemical polyphenols present in the leaf extract, which are essential for the ligation of transition metal ions to form metal oxide nanocrystals (Vanathi et al 2014 , Yuvakkumar et al. 2014) . During the reaction, a yellowcolored precipitate was obtained as an initial conformation. Th e precipitate was washed several times with ethanol, followed by calcination at 450 ° C for 1 h in a furnace (AJEON Heating Industrial Co., LTD, Gyeonggi-do, South Korea). Finally, a white powder was obtained and used in subsequent experiments.
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2

Doping and Surface Treatments of IZO Films

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For lithium doping in IZO film, 16.8 mg of lithium nitrate (Sigma‐Aldrich, 99.99%) was added in the mixture of indium(III) nitrate hydrate and zinc nitrate hexahydrate in 1 mL of deionized water. For F‐doped IZO, 34.4 mg of indium(III) fluoride trihydrate (Sigma‐Aldrich, 99%) was mixed with 14.5 mg of zinc nitrate hexahydrate in deionized water. The solutions were mixed overnight at room temperature and coated on substrates using the same recipe for undoped IZO film described above. For hydrogen peroxide treatment, 30% H2O2 was coated on deposited IZO film and annealed at 120 °C for 5 min. UV–ozone treatment was carried out on IZO film with 365 nm UV optical source (365 nm, 22 mW m−2) for 15 min and ozone generating system (PSDP‐UVT, Novascan Co.) for 30 min. UV–O2 plasma treatment was also performed with weak O2 plasma (25 W, 65 sccm O2) for 10 min after UV light illumination on IZO film for 5 min.
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3

Synthesis of Zinc Oxide Nanoparticles from Lagenaria siceraria

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Zinc nitrate, Xylene, Ethanol and all the chemicals required for carrying out the experiment was procured from Sigma-Aldrich. Fresh L. siceraria was obtained from Vellore local market, Tamil Nadu, India. Zinc nitrate was obtained from Sigma Aldrich, India. All aqueous solutions were prepared using deionized water. All glass wares were cleaned with chromic acid followed by thorough washing with deionized water and then with acetone for prior use.
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4

Fabric Coating Protocol with Methylene Blue

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Chemicals of an analytical reagent grade, including wetting agent, Citric acid (C6H8O7), Zinc nitrate (Zn(NO3)2·6H2O), sodium chloride, and sodium hydroxide were supplier of Merck (Burlington, VT, USA). The commercial-grade methylene blue was purchased from a local scientific store in Faisalabad, Pakistan. The unprocessed woven cotton fabric was procured from Sapphire Textile limited, Sheikhupura, Punjab, Pakistan. The woven fabric had 100 ends, 85 picks and a density of 118 g/m2. The raw fabric was desized to remove starch, dirt, and other contaminants before the coating tests.
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5

Trace Metal Analysis using Voltammetry

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The chemicals used in the present work were of analytical reagent grade and used as received, unless stated otherwise. All solutions were prepared with ultra-pure water (18.3 MΩ cm, Milli-Q systems, Millipore-waters). The nitric acid 65% (suprapur) and the standard stock solutions of mercury nitrate (1,001 ± 2 mg L−1), cadmium nitrate (999 ± 2 mg L−1), lead nitrate (999 ± 2 mg L−1), and zinc nitrate (1,000 ± 2 mg L−1) were purchased from Merck. Cd(II), Pb(II), and Zn(II) solutions were prepared from dilution of the certified standard. Ludox® TM40 [40% (w/w) suspension in water] and LS30 [30% (w/w) suspension in water] colloidal silica (SiO2) were purchased from Aldrich. Sodium nitrate electrolyte solution (10, 30, and 100 mM), MES [2-(N-morpholino)ethanesulfonic acid] buffer (200 mM), and MOPS [3-(N-morpholino)propanesulfonic acid] buffer (200 mM) were prepared from solids (Merck, suprapur and Merck >99%, respectively). The pH adjustments were performed using nitric acid (Merck, suprapur) and sodium hydroxide (100 mM standard, Merck) solutions.
Potassium thiocyanate, hydrochloric acid, and potassium chloride, all p.a. from Merck, were used to prepare the solution for the re-dissolution of the mercury film. Solutions of ammonium acetate [NH4CH3COO (1,000 mM)/CH3COOH (1,000 mM)] (Merck) were prepared monthly and used without further purification.
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6

Green Gram Seed Quality Evaluation

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Analytical-grade zinc nitrate
(98%), sodium hydroxide (99%), cysteine
(97%), and PVA (99%) were purchased from Merck. Genetically pure seeds
of the green gram variety CO 8 were used, which were procured from
the Department of Pulses, TNAU, Coimbatore. The seeds were washed,
dried, and size-graded using an 8×8 BSS sieve before being evaluated
in a lab for initial seed quality.
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7

Synthesis and Characterization of Dye-Adsorbing Nanomaterials

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Polyethylene glycol with average molecular weight 200 (PEG) (Sigma-Aldrich, Burlington, MA, USA, P3015), copper(II) nitrate (Merck, Darmstadt, Germany, 0140630), L-cysteine (Sigma-Aldrich, W326305), ammonium citrate dibasic (Fluka Analytical, 09831), ascorbic acid (Merck, F971227), zinc nitrate (Merck, CC396733), titanium(IV) isopropoxide (Aldrich Chemistry, 87560), isopropyl alcohol (Merck, 1.09634) and sodium chloride (Fluka, S9888), reagents of analytical grade, were acquired from Sigma-Aldrich. The dye references were: Methyl Orange (Fluka, 68250), Orange II sodium salt (Aldrich, 195235), Reactive Black 5 (Sigma-Aldrich, 306452) and Remazol Brilliant Blue R (Sigma, R8001), acquired from Sigma-Aldrich. The chemical structures of the dyes are shown in Figure 1.
A typical real textile effluent was obtained from a textile dying and printing industry located in Barcelos (Portugal), with the following features: COD, 339 ± 9 mg O2/L; turbidimetry, 57.7 ± 0.3 NTU; pH, 8.66 ± 0.01; conductivity, 4.61 ± 0.01 mS/cm; chloride anion, 100 mg/L; carbonates, >356 mg/L. Moreover, optical microscopy analysis of the raw textile effluent showed the presence of a considerable number of textile fibers, microplastics and microorganisms (raw effluent is a mixture of industrial effluent and sanitary sewage).
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8

Chitosan-Graphite Composite Synthesis

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Chitosan and graphite were purchased from Kimiya Azma and Black Diamond Companies, Iran, respectively. All other chemicals including cetyltrimethylammonium bromide (CTAB), tetraethylorthosilicate (TEOS), zinc nitrate, and ammonia solution were purchased from Merck Company.
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9

Heavy Metal Ions Removal from Wastewater

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All chemicals, lead nitrate (Pb(NO3)2), Zinc nitrate (Zn(NO3)2), Cadmium nitrate (Cd(NO3)2), Nickel nitrate (Ni(NO3)2), potassium permanganate (KMnO4), Hydrogen Peroxide (H2O2), Sodium hydroxide (NaOH), nitric acid (HNO3), Zinc sulfate heptahydrate (ZnSO4.7H2O), and potassium hydroxide (KOH) were provided by Merck company (Germany) and used without any further purification.
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10

Zinc-Based Inorganic Complexes Synthesis

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All reagents were used as received
without further purification. Zinc chloride (>98%, ZnCl2) and zinc nitrate (>99%, Zn(NO3)2(H2O)6) were purchased from Merck KGaA (Darmstadt,
Germany).
DAT (>98%, C2H5N5) was purchased
from Acros Organics (Geel, Belgium), and anhydrous ethanol (99.9%)
was purchased from Dae Jung Chemicals & Metals (Seoul, Korea).
HeLa, NCCIT, and MCF-7 cell lines were purchased from the American
Type Culture Collection (Manassas, Virginia).
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