To detect the proliferation of cultured HUVECs after stimulation, EdU (1 μM) was added to the culture medium 4 h before the end point of 24 h stimulation. EdU staining was then performed according to the manufacturer’s instructions of EdU Apollo488 In Vitro Kit (Ribobio) and analyzed by High-Content Imaging System (ImageXpress Micro, Molecular Device, USA).
Edu apollo488 in vitro kit
Manufactured by RiboBio
Sourced in United States
The EdU Apollo488 In Vitro Kit is a laboratory product designed for the detection and analysis of cell proliferation. It utilizes a fluorescent labeling technique to identify cells undergoing DNA synthesis, a key indicator of cell division and proliferation.
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3 protocols using edu apollo488 in vitro kit
1
Quantifying HUVEC Proliferation via EdU
2
EdU Proliferation Assay Protocol
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EdU experiment was conducted using the cell-light EdU Apollo 488 In Vitro kit (C10310-3; RiboBio). In brief, cells were fixed with 4 % paraformaldehyde (P0099; Beyotime) and permeated with 0.5 % Triton X-100 after EdU staining (50 nM) and stained with 1×Apollo® fluorescent dye. The nuclei were restained with DAPI. EdU-positive cells were photographed using FSX100 microscope (Olympus).
3
Analyzing Apoptosis and Proliferation in HCT116 Cells
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The apoptosis and proliferation rate of HCT116 cells were detected using annexin V fluorescein isothiocyanate (FITC) detection kit (556547; BD Biosciences, Franklin Lake, NJ, USA) and cell light EdU Apollo 488 in vitro Kit (C10310-3; Ribobio, Guangzhou, China) according to the manufacturer’s instruction. Samples were analyzed using flow cytometry (BD FACSCalibur, BD Biosciences).
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