Jsm 7100f sem

Manufactured by JEOL

Sourced in Japan

The JEOL JSM-7100F is a Scanning Electron Microscope (SEM) designed for high-resolution imaging and analysis of a wide range of samples. It features a thermal field emission electron gun, high-resolution electron optics, and advanced imaging and analytical capabilities.

Automatically generated - may contain errors

Lab products found in correlation

Pp3010t cryo sem preparation system, by Quorum Technologies (3 mentions) Dm2500 microscope, by Leica (3 mentions) Jsm 6340f, by JEOL (2 mentions) Nis elements software, by Nikon (1 mentions) A1r confocal microscope, by Nikon (1 mentions) Mc170 hd color camera, by Leica (1 mentions) Cary 60 uv vis spectrometer, by Agilent Technologies (1 mentions) Tm4000, by Hitachi (1 mentions) Ct 1000c cryo transfer system, by Oxford Instruments (1 mentions) Ir prestige 21, by Shimadzu (1 mentions) Asap 2020 analyzer, by Micromeritics (1 mentions)

13 protocols using jsm 7100f sem

Evaluating Phosphate Suspensions Produced from Calcium-Based Materials

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Each 0.2 g of BiSCaO-6, BiSCaO-2000 or SSP-Ca(OH)₂ to 100 mL of pure water, followed by rotary mixing, generated 0.2 wt% each water suspension. Then, 0.12 wt% Na₃PO₄, Na₂HPO₄, or NaH₂PO₄ was added to each suspension. The amount of H₃PO₄ were adjusted to be around pH 12. After rotary mixing, pH, average diameter, zeta potential, and form of each suspension were evaluated. Average diameter and zeta potential of particles were measured by ELSZ-1000 (Otsuka Electronics Co. Ltd., Osaka, Japan) [33 (link),34 ].
For scanning electron microscope (SEM) images of dry powder, after osmium metal coating using a neo-osmium coater (Neoc-STB; Meiwafosis Co., Ltd., Tokyo, Japan), the surface structure of each dry powder was observed with SEM images of a field-resolved scanning electron microscope (JSM-6340F; JEOL Ltd., Tokyo, Japan). For cryo-SEM, samples were frozen in liquid nitrogen, then knife-cut and observed in JEOL JSM 7100F SEM (JEOL Ltd., Tokyo, Japan) under vacuum conditions at minus 90 degrees. The accelerating voltage was 10 KV, and the detection signal was a backscattered electron image.

Sato Y., Ishihara M., Nakamura S., Fukuda K., Takayama T., Hiruma S., Murakami K., Fujita M, & Yokoe H. (2019). Preparation and Application of Bioshell Calcium Oxide (BiSCaO) Nanoparticle-Dispersions with Bactericidal Activity. Molecules, 24(18), 3415.

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Microstructure Analysis of Materials

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The microstructure was studied using Leica DM2500 microscope (Leica Microsystems, Belgium) under normal and polarized light. For confocal microscopy, samples were imaged using a Nikon A1R confocal microscope (Nikon Instruments Inc., USA). Excitation was performed by means of a 488 nm Ar laser and fluorescence was detected through a 525/50 bandpass filter. Images were acquired and processed with Nikon NIS Elements software. For cryo-SEM, samples were placed in the slots of a stub, plunge-frozen in liquid nitrogen, and transferred into the cryo-preparation chamber (PP3010T Cryo-SEM Preparation System, Quorum Technologies, UK) where it was freeze-fractured and subsequently sputter-coated with Pt and examined in JEOL JSM 7100F SEM (JEOL Ltd., Tokyo, Japan). For water containing samples, sublimation step was included to get rid of water.

Patel A.R, & Dewettinck K. (2015). Comparative evaluation of structured oil systems: Shellac oleogel, HPMC oleogel, and HIPE gel. European Journal of Lipid Science and Technology, 117(11), 1772-1781.

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Microstructure Analysis of Emulsions

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Optical and cryo-scanning electron microscopy (cryo-SEM) was utilized to study the microstructure of the samples. Optical microscopy was done on Leica DM2500 microscope (Leica Micro-systems, Belgium). For cryo-SEM, samples of the emulsions were placed in the slots of a stub, plunge-frozen in slush nitrogen and transferred into the cryo-preparation chamber (PP3010T cryo-SEM Preparation System, Quorum Technologies, UK) where they were freeze-fractured, sublimated for 20 min and subsequently sputter-coated with Pt and examined by a JEOL JSM 7100F SEM (JEOL Ltd, Tokyo, Japan).

Gao Z., Daneva A., Salanenka Y., Van Durme M., Huysmans M., Lin Z., De Winter F., Vanneste S., Karimi M., Van De Velde J., Vandepoele K., Van de Walle D., Dewettinck K., Lambrecht B.N, & Nowack M.K. (2018). KIRA1 and ORESARA1 terminate flower receptivity by promoting cell death in the stigma of Arabidopsis. Nature plants, 4(6), 365-375.

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Wax Crystal Microstructure Visualization

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The microstructure of wax crystals was observed under normal and polarized light (PLM) using a Leica DM2500 microscope (Wetzlar, Germany) equipped with a Leica MC170 HD color camera. For cryo-scanning electron microscopy (cryo-SEM), deoiling of organogel samples was carried out using butanol to remove the surface liquid oil in order to visualize the wax crystals. Precisely, the deoiling was carried out in two different ways: (1) A known quantity of organogel was weighed in a glass vial followed by addition of butanol (at a gel:butanol ratio of 1:50 w/w approximately), which led to the collapse of the gel structure and resultant sedimentation of the crystalline fraction. After overnight storage, the supernatant liquid was decanted to collect the sediment, which was then placed on the sample holder. (2) The organogel sample was directly placed on a specialized stub (sample holder with grooves) followed by a dropwise addition of butanol to remove the liquid oil. The stub was left for overnight drying in inverted position to drain out all the solvent. The stub was then plunge-frozen in liquid nitrogen and transferred into the cryo-preparation chamber (PP3010T Cryo-SEM Preparation System, Quorum Technologies, UK), where it was freeze-fractured and subsequently sputter-coated with Pt and examined in a JEOL JSM 7100F SEM (JEOL Ltd., Tokyo, Japan).

Patel A.R., Babaahmadi M., Lesaffer A, & Dewettinck K. (2015). Rheological profiling of organogels prepared at critical gelling concentrations of natural waxes in a triacylglycerol solvent. Journal of agricultural and food chemistry, 63(19).

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Cryo-SEM Visualization of Carrot Serum Pectin

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Cryo-scanning electron microscopy (cryo-SEM) was used to visualize the microstructure of carrot serum pectic polysaccharides in solution. Approximately 1 mg of lyophilized sample was dissolved overnight in 1 mL ultrapure water (organic free, 18 MΩ cm resistance). A few drops of the sample was placed onto a slot on a stub with rivets, vitrified
and transferred into the cryo-stage at -140 °C in the cryo-preparation chamber (PP3010T cryo-SEM preparation system, Quorom Technologies, UK). The sample was freeze-fractured, sublimated at -90 °C for 25-30 min under controlled vacuum conditions and then sputter coated with platinum using argon gas to prevent charging during electron beam targeting (Kyomugasho et al., 2016) . Finally, the sample was transferred onto the SEM stage and examined using a JEOL JSM 7100F SEM (JEOL Ltd, Tokyo, Japan) for their microstructure in solution.

Santiago J.S.J., Kyomugasho C., Maheshwari S., Jamsazzadeh Kermani Z., Van de Walle D., Van Loey A.M., Dewettinck K, & Hendrickx M.E. (2018). Unravelling the structure of serum pectin originating from thermally and mechanically processed carrot-based suspensions. Food Hydrocolloids., 77.

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Cryo-SEM Visualization of Ca2+-Enriched Pectin

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Cryo-scanning electron microscopy (cryo-SEM) was employed to visualise the microstructure of the Ca 2+ -enriched pectin-based solutions used in the adsorption study as well as samples generated in a gelation study. A small amount of sample was transferred into a stub, vitrified with liquid nitrogen as described by Kyomugasho et al. (2016) . The sample was then fractured at -140 °C to allow visualisation of the topography (gel-structure) once the free water present was sublimated (for 15-30 min at -90 °C) under controlled vacuum conditions. The sample was subsequently sputtered with platinum using argon gas to prevent charging during electron beam targeting. Visualisation was then performed on a SEM stage (JEOL JSM 7100F SEM, JEOL Ltd, Tokyo, Japan) at -140 °C (Patel et al., 2015) .

Kyomugasho C., Munyensanga C., Celus M., Van de walle D., Dewettinck K., Van Loey A.M., Grauwet T, & Hendrickx M.E. (2018). Molar mass influence on pectin-Ca2+ adsorption capacity, interaction energy and associated functionality: Gel microstructure and stiffness. Food Hydrocolloids., 85.

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Scanning Electron Microscopy of Dry Powders

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Scanning electron microscopy (SEM) images of the dry powders were obtained by osmium metal coating using a neo-osmium coater (Neoc-STB; Meiwafosis Co., Ltd., Tokyo, Japan). The surface structure of each dry powder was observed from SEM images obtained using a field-resolved scanning electron microscope (JSM-6340F; JEOL Ltd. Tokyo, Japan). For cryo-SEM [22 (link),23 (link)], a 0.2 wt.% BiSCaO colloidal dispersion containing 0.15 wt.% PP was frozen in liquid nitrogen, then knife-cut and observed using a JEOL JSM 7100F SEM (JEOL Ltd., Tokyo, Japan) under vacuum conditions at −90 °C. The accelerating voltage was 10 KV, and the detection signal was a backscattered electron image.

Sato Y., Ohata H., Inoue A., Ishihara M., Nakamura S., Fukuda K., Takayama T., Murakami K., Hiruma S, & Yokoe H. (2019). Application of Colloidal Dispersions of Bioshell Calcium Oxide (BiSCaO) for Disinfection. Polymers, 11(12), 1991.

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Characterization of Graphene Oxide Flakes

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Horiba Scientific, SPEX NanoLog fluorescence spectrophotometer was used to generate GO emission spectra with excitation of 400 nm utilized in previous works^{46 (link)}. Absorbance spectra were measured with Agilent Technologies, Cary 60 UV–vis spectrometer in the range of 200–800 nm to assess sample concentration, with an extinction coefficient of 44.26 mL/μg*cm determined experimentally. NT-MDT Nano Solver AFM and JEOL, JSM-7100F SEM were utilized to measure the average flake size of GO samples ultrasonically processed for 0, 20, 30, 50 and 60 min respectively.

Campbell E., Hasan M.T., Pho C., Callaghan K., Akkaraju G.R, & Naumov A.V. (2019). Graphene Oxide as a Multifunctional Platform for Intracellular Delivery, Imaging, and Cancer Sensing. Scientific Reports, 9, 416.

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Microstructural Analysis of Dried and Cooked Beans

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The microstructure of dried beans and fresh beans was examined using scanning electron microscopy (SEM), Hitachi TM4000 (Hitachi High-Technologies, Tokyo, Japan). A section from the central part of the cotyledon’s cross-sectional axis was viewed at vacuum charged-up reduction (low) at an accelerating voltage of 10 kV at × 200 magnification by backscattered electron (BSE) imaging.
Cryogenic SEM (Cryo-SEM) was employed for the evaluation of cooked and rehydrated beans. A section of the cotyledon was attached to the specimen holder of a CT-1000C cryo transfer system (Oxford Instruments, Oxford, UK) and frozen in slush nitrogen. The sample was sublimed and coated with tungsten before being transferred to the microscopic stage of JSM-7100F SEM (JEOL, Tokyo, Japan) and viewed at an accelerating voltage of 10 kV at × 100 magnification.

Aravindakshan S., Nguyen T.H., Kyomugasho C., Buvé C., Dewettinck K., Van Loey A, & Hendrickx M.E. (2021). The Impact of Drying and Rehydration on the Structural Properties and Quality Attributes of Pre-Cooked Dried Beans. Foods, 10(7), 1665.

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Characterization of SBC and MSBC Materials

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The surface area, pore width and pore volume of SBC and MSBC were measured with a Micromeritics ASAP 2020 analyzer (USA). The crystalline structure analysis was conducted with D8 X-ray diffraction spectra (Bruker, Germany). Fourier-transform infrared spectroscopy (FTIR) spectra were obtained with IRprestige-21 (Shimadzu, Japan) using the KBr pellet method and examined in the 4000–400 cm^-1 region. The software named OMNIC was applied to analyze the FTIR spectra. The surface morphologies of the powder samples were analyzed by a JSM-7100F SEM (JEOL, Japan).

Yang L., He L., Xue J., Wu L., Ma Y., Li H., Peng P., Li M, & Zhang Z. (2019). Highly efficient nickel (II) removal by sewage sludge biochar supported α-Fe2O3 and α-FeOOH: Sorption characteristics and mechanisms. PLoS ONE, 14(6), e0218114.

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