We used RIPA buffer (CWbio) containing 0.1 mg/mL PMSF (Keygen), protease inhibitor, and Phospho‐stop to lyse cells individually (Roche). The WB approach was the same as in our prior study.14 The following Abs were used: anti‐RAC1 (Proteintech 24,072‐1‐AP), anti‐FAK (Proteintech 12,636‐1‐AP), anti‐CSF (Proteintech 17,762‐1‐AP), anti‐TXNIP (Proteintech 18,243‐1‐AP), anti‐ITGA2 (Abcam ab133557), anti‐PAK (Abcam ab40852), anti‐pPAK (Abcam ab40795), and anti‐pFAK (CST 3283).
Anti pak
Manufactured by Abcam
Sourced in United Kingdom
Anti-PAK is a primary antibody that targets the p21-activated kinase (PAK) protein. PAK is a serine/threonine protein kinase that plays a role in various cellular processes. The Anti-PAK antibody can be used for the detection and analysis of PAK in various applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti rac1, by Proteintech (1 mentions)
Anti fak, by Proteintech (1 mentions)
Anti txnip, by Proteintech (1 mentions)
Anti itga2, by Abcam (1 mentions)
Protease inhibitor, by Roche (1 mentions)
Phenyl methyl sulfonyl fluoride (pmsf), by Keygen Biotech (1 mentions)
Anti ampk, by Abcam (1 mentions)
Anti β actin, by Santa Cruz Biotechnology (1 mentions)
Anti phospho ampk, by Abcam (1 mentions)
2 protocols using anti pak
1
Cell Lysis and Immunoblotting for Protein Analysis
2
Signaling pathway antibody analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Anti-phospho-PAK and anti-phospho-paxillin antibodies were purchased from Millipore (Billerica, MA, USA). Anti-phospho-AMPK and anti-AMPK, and anti-PAK antibodies were purchased from Abcam (Cambridge, UK, USA). Anti-Vav3 and anti-β-actin antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Metformin and AICAR were obtained from Calbiochem (San Diego, CA, USA).
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