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Carbon coated copper grid

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Carbon-coated copper grids are a type of laboratory equipment used in electron microscopy. They provide a stable and conductive support for samples being analyzed under an electron beam. The carbon coating enhances the sample's conductivity, improving imaging quality and reducing charging effects.

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Lab products found in correlation

Jem 1400 electron microscope, by JEOL (2 mentions) Temcam f416 camera, by TVIPS (2 mentions) Whatman, by Cytiva (2 mentions) Hcs cell mask deep red, by Thermo Fisher Scientific (1 mentions) Mitotracker red cmxros, by Thermo Fisher Scientific (1 mentions) Sucrose, by Avantor (1 mentions) Hoechst 33342 trihydrochloride trihydrate, by Thermo Fisher Scientific (1 mentions) Omnipaque, by GE Healthcare (1 mentions) Spectrum 100 spectrometer, by PerkinElmer (1 mentions) Jem 1400 tem, by JEOL (1 mentions) Us1000 ccd camera, by Ametek (1 mentions) Ems100 glow discharge unit, by Electron Microscopy Sciences (1 mentions) Cm200 feg, by Ametek (1 mentions) Cm100, by Ametek (1 mentions) No 3 filter paper, by Cytiva (1 mentions) Jem 2100, by JEOL (1 mentions) Ua zero em stain, by Agar Scientific (1 mentions) H 7650, by Hitachi (1 mentions) Eagle 2k ccd camera, by Thermo Fisher Scientific (1 mentions) Tecnai t12 microscope, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Copper grids (21 citations) Formvar carbon-coated copper grids (12 citations) Carbon-coated 400 mesh copper grid (9 citations) Holey carbon-coated copper grid (5 citations) Carbon-coated grids (4 citations) Carbon coated copper TEM grid (4 citations) 300 mesh carbon/formvar coated copper grid (2 citations) Carbon-coated 200 mesh copper specimen grid (2 citations) 20mesh carbon coated copper grid (2 citations) 300 mesh carbon-coated copper TEM grid (2 citations)

16 protocols using carbon coated copper grid

1

Characterization of Iohexol Nanoparticles

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All the chemicals were obtained from Sigma Aldrich (St Louis, MO, USA) and used without further purification, unless otherwise specified. Iohexol (Omnipaque™) was obtained from GE Healthcare (UK). MitoTracker® Red CMXRos, Image-iT®-DEAD Green™ viability, HCS Cell Mask™ deep red and Hoechst 33342 (trihydrochloride, trihydrate) were purchased from Life Technologies (Oregon, USA). Sucrose was acquired from VWR International Ltd (England, UK) and paraformaldehyde solution 37–41% was acquired from Fisher Scientific (England, UK). A spectrum 100 spectrometer (PerkinElmer) was used to perform IR measurements. Dynamic light scattering (DLS) and Z-potential were measured using a Zetasizer Nanoseries spectrophotometer (Malvern instruments) at 25 °C. TEM samples were prepared on carbon-coated copper grids (200 mesh, Agar scientific) and TEM images were acquired using a Tecnai T20 instrument (FEI). Powder-XRD data were obtained on a Bruker D8 Advance powder diffractometer with a Cu Kα X-ray source (λ = 1.54058 Å) operating at 40 kV and 40 mA and a Sol-X detector.
Imberti C., Fedatto Abelha T., Yan Y., Lange J., Cui X., Szanda I., Goh V., Dailey L.A, & T. M. de Rosales R. (2020). Synthesis and in vivo evaluation of PEG-BP–BaYbF5 nanoparticles for computed tomography imaging and their toxicity. Journal of Materials Chemistry. B, 8(34), 7723-7732.
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2

Visualizing Curli Fiber Formation in E. coli

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Desalted CsgA was incubated at room temperature in the presence or absence of CsgC. Samples for negative stain EM were prepared at different time points following guanidinium removal, by applying 2 µL of desalted protein solution on carbon-coated copper grids (Agar Scientific). After an incubation of 1 minute a wash step with deionized water was performed before staining with 1% (w/v) uranyl acetate. The earliest time point for TEM imaging was standardized to 3.5 min after the start of the desalting. The samples were imaged using a JEM-1400 electron microscope (JEOL Ltd.) equipped with a LaB6 cathode and operated at 120 kV. Images were recorded with a 4096 × 4096 pixel CMOS TemCam-F416 camera (TVIPS GmbH). Image J was used to measure fiber widths, either on in vitro grown fibers or on native curli on E. coli UGB1236 (n= 100) 50 (link).
Sleutel M., Van den Broeck I., Van Gerven N., Feuillie C., Jonckheere W., Valotteau C., Dufrêne Y.F, & Remaut H. (2017). Nucleation and growth of a bacterial functional amyloid at single fiber resolution. Nature chemical biology, 13(8), 902-908.
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3

Negative Staining of Protein Samples

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Carbon-coated copper grids (Agar Scientific, UK) were glow-discharged before application of 5 μL samples which were blotted after 30 s. Grids were stained twice with 2%(w/v) uranyl acetate and blotted dry, before viewing in a Jeol JEM-1400 TEM. Images were acquired at 40,000x magnification on a Gatan US1000 CCD camera.
Chandler-Bostock R., Mata C.P., Bingham R.J., Dykeman E.C., Meng B., Tuthill T.J., Rowlands D.J., Ranson N.A., Twarock R, & Stockley P.G. (2020). Assembly of infectious enteroviruses depends on multiple, conserved genomic RNA-coat protein contacts. PLoS Pathogens, 16(12), e1009146.
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4

Phage Morphological Characterization via TEM

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Ten microliters of the phage suspension (>1 × 1010 PFU/mL) was stained with 2.0% (w/v) uranyl acetate and pipetted onto carbon-coated copper grids (Agar Scientific, Stansted, UK) that were discharged using a EMS100 Glow Discharge Unit (Electron Microscopy Sciences, Hatfield, PA, USA). Stained samples were viewed using a FEI Tecnai F20 transmission electron microscope (ThermoFisher Scientific, Eindhoven, The Netherlands), fitted with a DE-16 camera (Direct Electron, San Diego, CA, USA). Images of the phages were taken at the University of Cape Town’s Electron Microscopy Unit (Cape Town, South Africa).
Aaron J., van Zyl L.J, & Dicks L.M. (2022). Isolation and Characterization of Lytic Proteus Virus 309. Viruses, 14(6), 1309.
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5

Polymer/Peptide Visualization via TEM

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Transmission electron microscopy (TEM) was used to image the polymer/peptide solutions as previously reported.23 (link) Peptide and polymer/peptide solutions were formed as previously described, however at a concentration of 0.5 mg/ml. Carbon-coated copper grids (400 mesh, Agar scientific) were glow discharged and placed shiny side down on the surface of a 10 ul droplet of sample for 10 seconds. The grids were placed on a 10 μl droplet of double deionized water for 10 seconds and blotted. Washed grids were placed on a 10 μl droplet of prepared and filtered uranyl acetate solution (4% w/v) for 60 seconds for the purposes of negative staining. This was blotted against double folded filter paper. Images were taken with a Tecnai G2 F20 Supertwin TEM operating at 200 kV.
Lee J., Zhao T., Peeler D.J., Lee D.C., Pichon T.J., Li D., Weigandt K.M., Horner P.J., Pozzo L.D., Sellers D.L, & Pun S.H. (2020). Formulation of thrombin-inhibiting hydrogels by self-assembly of ionic peptides with peptide-modified polymers. Soft matter, 16(15), 3762-3768.
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6

Visualizing Protein Folding on Lipid Monolayers

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Integrity and folding of all ILY variants were assessed by ring and arc formation on lipid monolayers. 10 µg/ml ILY and 1.6 µg/ml CD59, final concentrations, were mixed in Buffer A in a multi-well Teflon plate. The protein mixture was then overlaid with 2 μl of chloroform containing 1 mg/ml DOPC:cholesterol (1:1 molar ratio) and incubated at room temperature for 2 min to allow chloroform evaporation. Carbon-coated copper grids (Agar Scientific) were applied to the top of each well before incubattion at 37 °C for 15 min in a sealed petri dish with a Buffer A-soaked paper towel, to prevent evaporation. Grids were then stained with 2% uranyl acetate and imaged, as described earlier (Negative-stain EM).
Shah N.R., Voisin T.B., Parsons E.S., Boyd C.M., Hoogenboom B.W, & Bubeck D. (2020). Structural basis for tuning activity and membrane specificity of bacterial cytolysins. Nature Communications, 11, 5818.
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7

Transmission Electron Microscopy Sample Preparation

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Samples were applied to carbon-coated copper grids (400 mesh, Agar Scientific) after glow-discharging in air. Samples were stained with 0.75% (w/v) uranyl formate or 2% (w/v) phosphotungstic acid. For ice embedding, the sample was applied to a Vitrobot system (FEI), blotted, and frozen in liquid ethane. Imaging of stained samples was performed at a magnification of ∼40,000 on a Philips CM100 at 100 kV, incorporating a Gatan MultiScan 794 1K × 1K charge-coupled device camera. For low-dose (<10 e−/Å2) cryoimaging, micrographs were recorded at a magnification ∼60,000 on a Philips CM200 FEG operating at 200 kV in conjunction with a Gatan MegaScan 795 2K × 2K charge-coupled device camera.
Yamashita M., Shepherd M., Booth W.I., Xie H., Postis V., Nyathi Y., Tzokov S.B., Poole R.K., Baldwin S.A, & Bullough P.A. (2014). Structure and Function of the Bacterial Heterodimeric ABC Transporter CydDC: STIMULATION OF ATPASE ACTIVITY BY THIOL AND HEME COMPOUNDS. The Journal of Biological Chemistry, 289(33), 23177-23188.
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8

TEM Imaging of Protein Samples

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400 mesh carbon-coated copper grids (Agar Scientific) were glow discharged (25 mA for 20 seconds) before sample application. Approximately 3 μL SLH (100 ng/μL concentration) was applied to the grids for one minute. Excess liquid was blotted away using Whatman® No. 3 Filter paper before washing the grids twice with ultrapure water and blotting excess liquid. Staining was performed with 10 μL of UA-Zero® EM stain (Agar Scientific) for one minute before blotting away the excess. Grids were stored in a grid box at room temperature until imaged. Samples were imaged using a JEOL JEM-2100 transmission electron microscope operating at 200 kV.
Pasqualetto G., Mack A., Lewis E., Cooper R., Holland A., Borucu U., Mantell J., Davies T., Weckener M., Clare D., Green T., Kille P., Muhlhozl A, & Young M.T. (2023). CryoEM structure and Alphafold molecular modelling of a novel molluscan hemocyanin. PLOS ONE, 18(6), e0287294.
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9

Phage Morphology Analysis by TEM

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Transmission electron microscopy was used for determination of phage morphology. A filtered high-titer (~1011 PFU/mL) phage stock solution was placed on the surface of carbon-coated copper grids (Agar Scientific, Essex, UK). The phage was negatively stained using 2% (w/v) uranyl acetate, which was removed after 2 min. The grids with adsorbed phages were air dried, and the morphology of the phage was observed by transmission electron microscopy (Hitachi H-7650, Tokyo, Japan) at an acceleration voltage of 80 kV using a charge-coupled device camera (AMT400, Woburn, MA, USA).
Liu Y., Liu M., Hu R., Bai J., He X, & Jin Y. (2021). Isolation of the Novel Phage PHB09 and Its Potential Use against the Plant Pathogen Pseudomonas syringae pv. actinidiae. Viruses, 13(11), 2275.
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10

Visualizing Curli Fiber Formation in E. coli

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Desalted CsgA was incubated at room temperature in the presence or absence of CsgC. Samples for negative stain EM were prepared at different time points following guanidinium removal, by applying 2 µL of desalted protein solution on carbon-coated copper grids (Agar Scientific). After an incubation of 1 minute a wash step with deionized water was performed before staining with 1% (w/v) uranyl acetate. The earliest time point for TEM imaging was standardized to 3.5 min after the start of the desalting. The samples were imaged using a JEM-1400 electron microscope (JEOL Ltd.) equipped with a LaB6 cathode and operated at 120 kV. Images were recorded with a 4096 × 4096 pixel CMOS TemCam-F416 camera (TVIPS GmbH). Image J was used to measure fiber widths, either on in vitro grown fibers or on native curli on E. coli UGB1236 (n= 100) 50 (link).
Sleutel M., Van den Broeck I., Van Gerven N., Feuillie C., Jonckheere W., Valotteau C., Dufrêne Y.F, & Remaut H. (2017). Nucleation and growth of a bacterial functional amyloid at single fiber resolution. Nature chemical biology, 13(8), 902-908.
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