α neun

Manufactured by Merck Group

α-NeuN is an antibody that specifically binds to the neuronal nuclei antigen (NeuN), a DNA-binding, neuron-specific protein found in the nuclei of most neuronal cell types. This antibody is commonly used as a marker for identifying and quantifying neuronal cells in various research applications.

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Lab products found in correlation

α actin, by Merck Group (1 mentions) α annexin a1, by Abcam (1 mentions) α calnexin, by BD (1 mentions) α cd63, by Santa Cruz Biotechnology (1 mentions) α gapdh, by Cell Signaling Technology (1 mentions) α gfap, by Agilent Technologies (1 mentions) α actin, by Cytoskeleton (1 mentions) α rfp, by Abcam (1 mentions) Hrp conjugated secondary antibody, by Southern Biotech (1 mentions) α β actin, by Merck Group (1 mentions) α tubulin, by Merck Group (1 mentions) α mfsd2a, by Cell Signaling Technology (1 mentions) Mab377, by Abcam (1 mentions)

2 protocols using α neun

Antibody Characterization for Western Blot

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All antibodies used in this study are listed here, and concentrations used for specific experiments are listed in the relevant subsections as follows: α-actin (Millipore, MAB1501R), α-actin (Cytoskeleton Inc., AAN02), α-ALDH1L1 (Abcam, ab177463), α–annexin A1 (Abcam, ab214486), α-calnexin (BD Biosciences, 610523), α-CD63 (Santa Cruz Biotechnology, 5275), α-CD81 (BD Biosciences, 555675), α-FLAG (Millipore, F7425), α-FLAG-M2 (Millipore, F1804), α-GAPDH (α–glyceraldehyde-3-phosphate dehydrogenase; Cell Signaling Technology, 8884), α-GFAP (Millipore, MAB360), α-GFAP (DAKO, Z0334), α-GLAST (39 (link)), α-GLT1 (39 (link)), α-MCT4 (Proteintech, 22787-1-AP), α-NeuN (Millipore, MAB377), α-RFP (α–red fluorescent protein; Abcam, ab62341), α-S100β (DAKO, Z0311), α-WAVE2 (Cell Signaling Technology, 3659), and α-WAVE3 (Cell Signaling Technology, 2806).

Levy-Myers R., Daudelin D., Na C.H, & Sockanathan S. (2023). An independent regulator of global release pathways in astrocytes generates a subtype of extracellular vesicles required for postsynaptic function. Science Advances, 9(25), eadg2067.

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Western Blot Analysis of Neural Markers

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Protein lysate samples were boiled at 95°C, run on 6–12% polyacrylamide gels, and electrophoretically transferred onto PVDF membranes. Membranes were blocked with 5% BSA/TBST (0.1% Tween-20) or 5% non-fat milk/TBST and incubated overnight at 4°C with the following primary antibodies: α-Mfsd2a (1:1000, Cell Signaling Technologies; RRID:AB_2617168), α-NeuN (1:2000, Millipore #MAB377; RRID:AB_2298772), α-VE-Cadherin (1:1000, Abcam #ab33168; RRID:AB_870662), α-Aquaporin-4 (1:1000, Millipore #ABN411; RRID:AB_2637077), α-PDGFR-β (1:1000, Cell Signaling Technologies #3169; RRID:AB_2162497), α-β-Actin (1:5000, Sigma Aldrich #A2228; RRID:AB_476697), and α-Tubulin (1:8000, Sigma Aldrich #T5168; RRID:AB_477579). Membranes were then incubated with the appropriate HRP-conjugated secondary antibody (1:10000, Southern Biotech) and developed with regular or super ECL (Thermo Fisher Scientific). The intensity of individual bands was quantified using ImageJ.

Andreone B.J., Chow B.W., Tata A., Lacoste B., Ben-Zvi A., Bullock K., Deik A.A., Ginty D.D., Clish C.B, & Gu C. (2017). Blood-brain barrier permeability is regulated by lipid transport-dependent suppression of caveolae-mediated transcytosis. Neuron, 94(3), 581-594.e5.

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