Tetracycline tc

Manufactured by Merck Group

Sourced in United States

Tetracycline (TC) is a broad-spectrum antibiotic used in laboratory settings. It functions as an inhibitor of bacterial protein synthesis, preventing the growth and reproduction of various microorganisms.

Automatically generated - may contain errors

Lab products found in correlation

Penicillin streptomycin p s, by Thermo Fisher Scientific (4 mentions) Sh sy5y cell, by American Type Culture Collection (4 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions) Dulbecco modified eagle medium (dmem), by American Type Culture Collection (4 mentions) Fetal bovine serum (fbs), by American Type Culture Collection (2 mentions) Lc 20a, by Shimadzu (1 mentions) Nalidixic acid, by Merck Group (1 mentions) 4 hydroxybenzaldehyde, by Sangon (1 mentions) Vanillin, by Sangon (1 mentions) Dmem medium, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions) Tryptone soya medium, by Thermo Fisher Scientific (1 mentions) Lysogeny broth medium, by Thermo Fisher Scientific (1 mentions) Ciprofloxacin, by Merck Group (1 mentions) Spectinomycin, by MP Biomedicals (1 mentions) Erythromycin em, by Merck Group (1 mentions) Cytarabine, by Merck Group (1 mentions) Penicillin streptomycin p s, by Merck Group (1 mentions) Trypsin, by Merck Group (1 mentions) Penicillin streptomycin, by Merck Group (1 mentions)

Close spelling variants of this product

Tetracycline hydrochloride (TC) Tetracycline

13 protocols using tetracycline tc

SDZ 30-1 Binding Affinity Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

The SDZ 30-1 (5′-AACCCAATGGGAT-3′, K_d = 65.72 nM) was selected in our laboratory, which was purified using HPLC and synthesized by Sangon Biotech, Shanghai, China [24 (link)]. All antibiotics, e.g., SDZ, tetracycline (TC), furaltadone (FTD), and norfloxacin (OFL) were bought from Sigma-Aldrich, St. Louis, MO, USA. KMnO₄, hexadecyl trimethyl ammonium bromide (CTAB), buffer, and other reagents were purchased from Aladdin Co., Ltd., Ontario, CA, USA. The accuracy of the method was verified by HPLC on LC-20A (Shimadzu, Kyoto, Japan). Fluorescence intensity measurements were performed at the excitation wavelength of 492 nm and emission wavelength of 518 nm using Varioskan LUX.

Zheng X., Yang L., Sun Q., Zhang L, & Le T. (2023). Development and Validation of Aptasensor Based on MnO2 for the Detection of Sulfadiazine Residues. Biosensors, 13(6), 613.

+ Open protocol

+ Expand

Culturing MC65 and SH-SY5Y Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

MC65 cells (kindly provided by Dr. George M. Martin at the University of Washington, Seattle, WA, USA) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Life Technologies, Inc., Grand Island, NY, USA) supplemented with 10% of heat-inactivated fetal bovine serum (FBS) (Hyclone, Logan, UT, USA), 1% Penicillin/Streptomycin (P/S) (Invitrogen, Carlsbad, CA, USA), 1 μg/mL Tetracycline (TC) (Sigma Aldrich, St. Louis, MO, USA), and 0.2 mg/mL G418 (Invitrogen). SH-SY5Y cells (ATCC) were cultured in DMEM supplemented with 10% FBS and 1% P/S. All cells were maintained at 37 °C in a fully humidified atmosphere containing 5% CO₂.

Saathoff J.M., Liu K., Chojnacki J.E., He L., Chen Q., Lesnefsky E.J, & Zhang S. (2016). Mechanistic Insight of Bivalent Compound 21MO as Potential Neuroprotectant for Alzheimer’s Disease. Molecules, 21(4), 412.

+ Open protocol

+ Expand

Culturing MC65 and SH-SY5Y Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

MC65 cells (kindly provided by Dr. George M. Martin at the University of Washington, Seattle) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Life Technologies, Inc., Grand Island, NY) supplemented with 10% of heat-inactivated fetal bovine serum (FBS) (Hyclone, Logan, UT), 1% Penicillin/Streptomycin (P/S) (Invitrogen), 1 μg/mL Tetracycline (TC) (Sigma Aldrich, St. Louis, MO), and 0.2 mg/mL G418 (Invitrogen). SH-SY5Y cells (ATCC) were cultured in DMEM supplemented with 10% FBS and 1% P/S. All cells were maintained at 37 °C in a fully humidified atmosphere containing 5% CO₂.

Liu K., Chojnacki J.E., Wade E.E., Saathoff J.M., Lesnefsky E.J., Chen Q, & Zhang S. (2015). Bivalent compound 17MN exerts neuroprotection through interaction at multiple sites in a cellular model of Alzheimer’s disease. Journal of Alzheimer's disease : JAD, 47(4), 1021-1033.

+ Open protocol

+ Expand

Construction of Z. mobilis Recombinants

Check if the same lab product or an alternative is used in the 5 most similar protocols

Zymomonas mobilis ZM4 (ATCC 31821) was purchased from American Type Culture Collection (Manassas, VA, USA). Shuttle vector pHW20a was used for construction of Z. mobilis recombinants [15 (link)]. Yeast extract was purchased from Oxoid (Hampshire, UK). Tetracycline (Tc) and nalidixic acid were from Sigma-Aldrich, St. Louis, MO, USA. 4-Hydroxybenzaldehyde, syringaldehyde, and vanillin were from Sangon Biotech Co., Shanghai, China. All other analytical grade chemicals were from Sinopharm Chemical Reagents, Shanghai, China.

Yi X., Gu H., Gao Q., Liu Z.L, & Bao J. (2015). Transcriptome analysis of Zymomonas mobilis ZM4 reveals mechanisms of tolerance and detoxification of phenolic aldehyde inhibitors from lignocellulose pretreatment. Biotechnology for Biofuels, 8, 153.

+ Open protocol

+ Expand

Inducible P53 Mutant Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

pLVX-Tet-On Advanced System was performed according to the manufacturer's instructions. As to the first stable transfection, the Hep3B cells were transfected with pLVX-Tet-On vector (Clonth) using Lipofecamine^TM2000 (Invitrogen), and then established the stable cell line by selecting and screening using 2mg/ml G418 (BD Biosciences Clontech). To ensure optimal induction and low background, multiple clonal cell lines must be screened after each stable transfection using western blotting. As to the Second stable transfection, pVSVG/ P53 (N340Q/L344R was infected into the pLVX-Tet-On Hep3B stable cell line, and transfected cells were grown in presence of 0.05 mg/ml Zeo (BD Biosciences Clontech) for selection. Individual colonies were isolated and screened for P53 (N340Q/L344R expression. The positive clones were cultured in DMEM medium (Gibco BRL Life Technologies) containing the indicated tetracycline (Tc) (sigma) concentrations (0-2μg/ml) to induce the P53 (N340Q/L344R expression.

Wu M., An J., Zheng Q., Xin X., Lin Z., Li X., Li H, & Lu D. (2016). Double mutant P53 (N340Q/L344R) promotes hepatocarcinogenesis through upregulation of Pim1 mediated by PKM2 and LncRNA CUDR. Oncotarget, 7(41), 66525-66539.

+ Open protocol

+ Expand

Antimicrobial Preservative Preparation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tryptone soya medium (agar, TSA; broth, TSB) and Lysogeny Broth (LB) medium were obtained from Oxoid Ltd. Tetracycline (Tc) and ciprofloxacin (CIP) antibiotics were supplied by Sigma-Aldrich; dimethyl sulfoxide (DMSO) and other analytical grade chemicals were supplied by Fisher Scientific. A minimal basal salts growth medium (BSM) was prepared as described by Rushton et al. [21 (link)] without casamino acids. A 3 : 1 cosmetics grade blend of chloromethylisothiazolinone and methylisothiazolinone (CMIT/MIT), and methylisothiazolinone (MIT) were obtained from Dow Chemical Company, USA; dimethyl dimethylol hydantoin (DMDMH) from Lonza, UK; benzisothiazolinone (BIT) and phenoxyethanol (PH) from Clariant International Ltd, Switzerland. Preservative stock solutions were prepared (in sterile distilled water or DMSO for PH) on the day of use and diluted as required.

Rushton L., Donoghue D., Bull M., Jay P, & Mahenthiralingam E. (2021). Construction and evaluation of a bioluminescent Pseudomonas aeruginosa reporter for use in preservative efficacy testing. Microbiology, 167(8), 001072.

+ Open protocol

+ Expand

Genetic Manipulation of S. aureus Strains

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The S. aureus strains tested in the murine gastrointestinal colonization model are listed in Table 3; each was resistant to Sm. Mutations in the S. aureus ica locus, spa, and atl were moved into Sm-resistant Newman by transduction with phage 80α or phage 85 from the original antibiotic marked mutant strains [42 (link)–44 (link)]. The tagO::tet mutation was moved from S. aureus RN4220 into Newman Δatl as described previously [22 (link)]. Mutants were confirmed by PCR or Southern blot analysis and were phenotypically identical to the parental strains in terms of the growth rate, hemolysis on sheep blood agar plates, and the metabolic profile on API Staph test strips (Biomerieux, Inc., Durham, NC). S. aureus strains were cultivated in TSB to the logarithmic growth phase, unless otherwise noted. Sm (0.5 mg/ml; Sigma Chemical Co., St. Louis, Mo.), erythromycin (Em; 5 μg/ml; Sigma), tetracycline (Tc; 2.5 μg/ml; Sigma), or spectinomycin (Spc; 100 μg/ml; MP Biomedicals, Solon, OH) was added to culture medium for selection where appropriate.

Misawa Y., Kelley K.A., Wang X., Wang L., Park W.B., Birtel J., Saslowsky D, & Lee J.C. (2015). Staphylococcus aureus Colonization of the Mouse Gastrointestinal Tract Is Modulated by Wall Teichoic Acid, Capsule, and Surface Proteins. PLoS Pathogens, 11(7), e1005061.

+ Open protocol

+ Expand

Culturing Neuronal and Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

MC65 cells were kindly provided by Dr. George M. Martin at the University of Washington, Seattle and were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Life Technologies, Inc., Grand Island, NY) supplemented with 10% of heat-inactivated fetal bovine serum (FBS) (Hyclone, Logan, UT), 1% penicillin/streptomycin (P/S) (Sigma-Aldrich), tetracycline (TC) (1 μg/mL, Sigma-Aldrich) and 0.2 mg/mL G418 (Invitrogen). SHSY5Y cells (ATCC, Manassas, VA) were cultured in DMEM supplemented with 10% FBS and 1% P/S. All cells were maintained at 37 °C in a fully humidified atmosphere containing 5% CO₂.
Cortex from E15.5 embryos of C57BL/6J mice were dissected then enzymatically dissociated in neurobasal medium containing trypsin (2.5 mg/mL, Sigma-Aldrich) and DNase I (15 μg/mL, Sigma-Aldrich) for 30 min at 37 °C, washed in neurobasal medium, then triturated using fire-polished Pasteur pipettes. Cell suspensions were passed through a 70 μm strainer to remove debris. Cells were plated on poly-D-lysine (Sigma Aldrich) coated plates or glass-bottomed dish and cultured in neuronal culture media (neurobasal medium supplemented with B27, GlutaMax (0.5 mM) and penicillin/streptomycin/amphotericin B). Neuronal culture media were changed by one-half volume exchange every 3 days. Cytarabine (1 μM final, Sigma-Aldrich) was added from day in vitro (DIV) 3 to 6 to inhibit glial proliferation.

Green J.C., Jiang Y., He L., Xu Y., Sun D., Keoprasert T., Nelson C., Oh U., Lesnefsky E.J., Kellogg G.E., Chen Q, & Zhang S. (2020). Characterization and discovery of a selective small molecule modulator of mitochondrial complex I targeting a unique binding site. Journal of medicinal chemistry, 63(20), 11819-11830.

+ Open protocol

+ Expand

Culturing MC65 Cells for Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

MC65 cells (kindly provided by Dr. George M. Martin at the University of Washington, Seattle) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Life Technologies, Inc., Grand Island, NY) supplemented with 10% of heat-inactivated fetal bovine serum (FBS) (Hyclone, Logan, UT), 1% Penicillin/Streptomycin (P/S) (Invitrogen), 1 μg/mL Tetracycline (TC) (Sigma Aldrich, St. Louis, MO), and 0.2 mg/mL G418 (Invitrogen). All assays were carried out in Opti-MEM Reduced Serum Medium (Life Technologies, Inc., Grand Island, NY). Cells were maintained at 37 °C in a fully humidified atmosphere containing 5% CO_2. Cell controls were prepared in Opti-MEM with or without TC (+TC or −TC).

Chojnacki J.E., Liu K., Saathoff J.M, & Zhang S. (2015). Bivalent ligands incorporating curcumin and diosgenin as multifunctional compounds against Alzheimer’s disease. Bioorganic & medicinal chemistry, 23(22), 7324-7331.

+ Open protocol

+ Expand

Minimum Inhibitory Concentration of Gram-negative Bacteria

Check if the same lab product or an alternative is used in the 5 most similar protocols

Minimum inhibitory concentration (MIC) values for different strains of Gram-negative bacteria are shown in Table 1. Strains were maintained on Luria–Bertani broth solid agar (1.5% agar, LA, Sigma-Aldrich Chemie GmbH, Steinheim, Germany) at 30 °C. Difco Nutrient Broth (NB) and Difco Nutrient Agar (NA) were used in experiments with AuHCl₄. Antibiotics were added to the medium in the following concentrations (µg/mL): ampicillin (Ap, OAO “Biochemist”, Moscow, Russia), 100; kanamycin (Km, OAO “Synthesis”, Moscow, Russia), 100; tetracycline (Tc, Sigma-Aldrich Chemie GmbH, Steinheim, Germany), 20–40, gentamicin (Gm, KRKA, Novo mesto, Slovenia), 40; and rifampicin (Rif, Sigma), 50. The chemicals used during this study: HAuCl₄·3H₂O (99.9%), NaBH₄ (99%), ascorbic acid (99%), cetyl-trimethyl-ammonium bromide (CTAB, 99%), trisodium citrate dihydrate, AgNO₃ (99%), and poly(ethylene glycol) PEG 40,000, were used as purchased (Sigma-Aldrich). TiO₂ Aeroxide^® P25 (Degussa P25, Frankfurt, Germany) was used as received.

Radzig M., Koksharova O., Khmel I., Ivanov V., Yorov K., Kiwi J., Rtimi S., Tastekova E., Aybush A, & Nadtochenko V. (2019). Femtosecond Spectroscopy of Au Hot-Electron Injection into TiO2: Evidence for Au/TiO2 Plasmon Photocatalysis by Bactericidal Au Ions and Related Phenomena. Nanomaterials, 9(2), 217.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!