Acetonitrile

Manufactured by Daejung Chemicals

Sourced in Cameroon, United States

Acetonitrile is a polar organic solvent commonly used in analytical chemistry and various industrial processes. It has a high solvating power and is often employed as a mobile phase component in High-Performance Liquid Chromatography (HPLC) and other analytical techniques. Acetonitrile is a colorless, volatile liquid with a characteristic odor.

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HPLC grade acetonitrile (5 citations) Acetonitrile (ACN) (2 citations)

23 protocols using acetonitrile

Analytical Characterization of Flavonoid Standards

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All solvents used in this study were of high-performance liquid chromatography (HPLC) grade. Water was obtained from J. T. Baker (Phillipsburg, NJ, USA); methanol, from Dunstan (Seoul, Korea); and acetonitrile, from Daejung (Gyonggi-do, Korea). Trifluoroacetic acid (TFA; extrapure grade) was supplied by Alfa Aesar (Ward Hill, MA, USA). Quercetin-3,4′-O-diglucoside and quercetin-4′-O-monoglucoside were supplied by Polyphenols Laboratories AS (Sandnes, Norway). The purity of the flavonol standards was controlled by HPLC and was >99%. Gallic acid, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Tro-lox), 2,4,6-tris (1-pyridyl)-5-triazine (TPTZ), ferric chloride, and Folin–Ciocalteu reagent were purchased from Sigma-Aldrich (St. Louis, MO, USA), and 2,2-diphenyl-1-picrylhydrazyl was purchased from Wako Pure Chemicals (Osaka, Japan) and were used for antioxidant assays. The saccharide standards sucrose (>99.5%), d-glucose (>99.5%), and d-fructose (guaranteed reagent grade) were obtained from Fluka (Buchs, Switzerland), Sigma-Aldrich (Buchs, Switzerland), and Junsei Chemical Co. (Koshigaya, Saitama, Japan), respectively.
The stock solutions of quercetin (1 mg/mL) and quercetin glucosides (4 mg/mL) were prepared in 75% ethanol. All solutions were stored at −20°C. Calibration of standards was obtained by appropriate dilution of the stock solutions.

Sharma K., Ko E.Y., Assefa A.D., Ha S., Nile S.H., Lee E.T, & Park S.W. (2014). Temperature-dependent studies on the total phenolics, flavonoids, antioxidant activities, and sugar content in six onion varieties. Journal of Food and Drug Analysis, 23(2), 243-252.

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Quantification of PVAc via HPLC

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The standard of PVAc (USP Reference Standard) was purchased from Sigma-Aldrich (Darmstadt, Germany). HPLC-grade water, methanol, and acetonitrile were obtained from Daejung Chemicals and Metals (Gyeonggi-do, Korea). A 0.2-μm polyvinylidene fluoride (PVDF) filter was obtained from Whatman (Maidstone, England).
Stock standard solution of PVAc was prepared in acetonitrile at 1000 mg/L and stored at −4 °C until use. Dilution of standard was used to prepare concentrations of 50, 100, 200, 300, 400, 500, 600, 700, 800, and 900 mg/L. All dilution standards were used immediately after preparation.

Sim S., Kim Y.M., Park Y.J., Siddiqui M.X., Gang Y., Lee J., Lee C, & Suh H.J. (2020). Determination of Polyvinyl Acetate in Chewing Gum Using High-Performance Liquid Chromatography–Evaporative Light Scattering Detector and Pyrolyzer–Gas Chromatography–Mass Spectrometry. Foods, 9(10), 1473.

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Formulation and Evaluation of Enzalutamide Soft Capsules

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ENZ was purchased from MSN Labratories Private Ltd. (Telangana, India). The Xtandi^® soft capsule, TPGS and MCT oil were supplied by Boryung Corporation (Seoul, Republic of Korea). Labrafac PG, Peceol, Labrasol, Capryol 90, Transcutol P, and Lauroglycol 90 were obtained from Gattefosse (St. Priest, France). Cremophor EL, Cremophor RH60, and Solutol HS15 were supplied by BASF (Ludwigshafen, Germany). Castor oil, coconut oil, olive oil, sesame oil, sunflower oil, corn oil, soybean oil, cotton seed oil, peanut oil, polysorbate 80, ammonium acetate, acetone, and acetonitrile were purchased from Daejung Co. Ltd. (Siheung, Republic of Korea). Sodium carboxymethyl cellulose was purchased from Duksan Pure Chemical (Ansan, Republic of Korea). All other chemicals used were of analytical grade.

Lee S.M., Lee J.G., Yun T.H., Cho J.H, & Kim K.S. (2024). Enhanced Stability and Improved Oral Absorption of Enzalutamide with Self-Nanoemulsifying Drug Delivery System. International Journal of Molecular Sciences, 25(2), 1197.

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Murine Lymphocyte Cytokine Profiling

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OVA and trifluoroacetic acid (TFA) were purchased from Sigma-Aldrich (Steinheim, Germany). HPLC grade water and acetonitrile were purchased from Daejung Chemical (Siheung-si, Gyeonggi-do, Republic of Korea). Spin-X centrifuge filter 0.45 μm with cellulose acetate was purchased from Costar (Corning Incorporated, Corning, NY, USA). The following FITC- or PE-conjugated monoclonal anti bodies (Abs) and non-labeled Abs were purchased from BD Bioscience (San Joes, CA, USA): FITC- IFN-γ, PE-CD8, and CD16/32 (2.4G2). The cytokine ELISA primary and secondary–antibodies specific for murine IL-2, IFN-γ were purchased from BD Biosciences (San Jose, CA, USA). The 5-Bromo-2 –Deoxy-Uridine Labeling and Detection Kit III, and collagenase D was purchased from Roche (Salt Lake City, UT, USA).

Lee S.J., Lee H.S., Hwang Y.H., Kim J.J., Kang K.Y., Kim S.J., Kim H.K., Kim J.D., Jeong D.H., Paik M.J, & Yee S.T. (2019). Enhanced anti-tumor immunotherapy by dissolving microneedle patch loaded ovalbumin. PLoS ONE, 14(8), e0220382.

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Enzymatic Synthesis of Glycopeptide Conjugates

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Leucine aminopeptidase (EC 3.4.1.2) microsomal from porcine kidney (LAP), β-glucuronidase (EC 3.2.1.31) from helix pomatia type H-2 (GLU), N-(tert-Butoxycarbonyl)glycine, glycine, triglycine, σ-pinene, triphosgene, triethylamine, N-carboxyanhydride (NCA), 2-aminoethyl methacrylate hydrochloride (2-AMEA), allylamine, 4-nitrophenyl- β-D-glucuronide (PNPG), hydroxybenzotriazole (HOBt), N,N’-diisopropylcarboiimide (DIC), acrylic acid N-hydroxysuccinimide (Ac–NHS ester), deuterium oxide (D₂O), dimethylsulfoxide-d₆ (DMSO-d), chloroform-d (CDCl₃), 2,2-dihydroxyindane-1,3-dione (Ninhydrin) and tris(hydroxymethyl)aminomethane hydrochloride (Tris–HCl) was purchased from Sigma Aldrich (St. Louis, MO, USA). GGRSK was custom-synthesized from Lifetein (Somerset, NJ, USA) and Cosmogenetech (Seoul, South Korea). Urokinase (EC 3.4.4.21)-type plasminogen activator (uPA) was purchased from Fortunachem (Wuhan, China). Acrylic acid (AAc), sodium hydroxide (NaOH), ethyl acetate anhydrous (EA), dimethylformamide (DMF), tetrahydrofuran (THF), chloroform (CCl₄), methanol (MeOH), n-butanol (BuOH), acetic acid, acetonitrile, and trifluoroacetic acid were purchased from Daejung (South Korea). 2-hydroxy-2-methlypropiophenone (Darocur 1173) and dimethylsulfoxide anhydrous (DMSO) were purchased from TCI (Tokyo, Japan).

Whang M., Yu H, & Kim J. (2021). Superabsorbent Polymer Network Degradable by a Human Urinary Enzyme. Polymers, 13(6), 929.

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Microbiological Media and Analytical Reagents for Research

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De Man, Rogosa, and Sharpe Broth (MRS broth), Tryptic Soy Broth (MB Cell, KisanBio Co., Ltd., Seoul, South Korea), Peptone and Agar (BD Difco, Sparks, MD, USA), ethanol, methanol, isopropanol, DMSO, acetonitrile, and nutritional media were acquired from Daejung Chemicals and Metals Co., Ltd., Siheung-si, Gyeonggi-do, South Korea. Sodium chloride (NaCl), monopotassium phosphate, dipotassium phosphate, Sodium chloride, sodium dihydrogen phosphate, sodium carbonate, potassium persulfate, sodium citrate, 2,2′-Azino-bis (3-ethyl benzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), methyl cellosolve, Folin–Ciocalteu reagent, triton-X100, Nile red, 5-fluoro-2′-deoxyuridine, β-carotene, quercetin, ferulic acid, gallic acids, p-coumaric acid, caffeic acid, catechin, and curcumin, lipase from porcine pancreas, dimethyl sulfoxide (DMSO), trolox (≥98% purity by HPLC), and butylated hydroxyanisole (BHA) (≥99%) were acquired from Sigma-Aldrich, Seoul, South Korea. PicoSens™ Triglyceride Assay Kit was acquired from BIOMAX, Korea. All of the reagents were of analytical quality.

Barathikannan K., Tyagi A., Shan L., Kim N.H., Lee D.S., Park J.S., Chelliah R, & Oh D.H. (2023). Antiobesity and Antioxidative Effect of Fermented Brown Rice Using In Vitro with In Vivo Caenorhabditis elegans Model. Life, 13(2), 374.

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Optimizing Gelatin-Alginate Hydrogel for EGF Delivery

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Gel strength 90–110 gelatin A (GA, average 20–25 kDa) and trifluoro acetic acid (TFA) were purchased from Sigma–Aldrich (St Louis, MO, USA). Sodium alginate (SA), acetic acid, and acetonitrile (ACN) were purchased from Dae Jung (Gyeonggi-do, South Korea). Epidermal growth factor (EGF) was provided by Daewoong Pharmaceutical Co., Ltd. Dulbecco’s phosphate buffered saline (DPBS) was purchased from Welgene (Gyeongsangbuk-do, South Korea), Dulbecco’s Modified Eagle Medium (DMEM) was obtained from Hyclone (Logan, UT, USA), 0.25% Trypsin-EDTA and fetal bovine serum (FBS) were purchased from Gibco (Paisley, UK).
HaCaT cells were purchased from Lonza (Walkersville, MD, USA). The cell counting kit-8 (CCK-8) was obtained from Dojindo (Kumamoto, Japan). Human EGF antibody as primary antibody (R&D systems, Minneapolis, MN, USA), rabbit anti-mouse horseradish peroxidase (HRP) as secondary antibody (Abcam, Cambridge, UK) were purchased.

Jeong S., Kim B., Park M., Ban E., Lee S.H, & Kim A. (2020). Improved Diabetic Wound Healing by EGF Encapsulation in Gelatin-Alginate Coacervates. Pharmaceutics, 12(4), 334.

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Extraction and Analysis of Plant Metabolites

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Absolute ethanol, allyl isothiocyanate, barium acetate, ß-mercaptoethanol, cetyltrimethylammonium bromide (CTAB), chloroform, ethylenediaminetetraacetic acid (EDTA 500 mM), formic acid (FA), isoamyl alcohol, lead(II) acetate, phenyl isothiocyanate, polyvinylpyrrolidone (PVP), sodium acetate, sodium chloride, and Tris–HCl pH 7.5 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Aryl sulfatase from Helix pomatia with 10,000 units (type H-1; EC 3.1.6.1) was obtained from Sigma-Aldrich. DEAE Sephadex A-25 was purchased from Cytiva (Marlborough, MA, USA). Glucotropaeolin potassium salt was obtained from Extrasynthese (Genay Cedex, Rhône, France). Plant Total RNA Mini Kit without DNase was purchased from Geneaid (New Taipei City, Taiwan). High-performance liquid chromatography (HPLC) solvents acetonitrile and methanol were purchased from Daejung (Republic of Korea), and water and dichloromethane were obtained from Thermo Fisher Scientific (Waltham, MA, USA).

Truong T.Q., Park Y.J., Jeon J.S., Choi J., Koo S.Y., Choi Y.B., Huynh P.K., Moon J, & Kim S.M. (2024). Myrosinase isogenes in wasabi (Wasabia japonica Matsum) and their putative roles in glucosinolate metabolism. BMC Plant Biology, 24, 353.

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HPLC Analysis of E2 in Samples

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All the mixture was filtrated by a PVDF filter (0.45 µm) and placed to a 1.5 ml HPLC vial for analysis. The analysis was done according to USP procedure for E2. Acetonitrile (Dae Jung, Korea) in ultrapure (55:45) was the mobile phase, flow rate was 1 ml/minute, and detection was done with UV detector at 205nm wavelength. A 3.9 mm × 30 cm, 5 µm, C18 column (Kromasil ® , Sweden) was used for separation. The sample injection amount was 25 µl and runtime was 15 minutes per samples.

Reda K., Rizwan F., Tanim M.M.R., & Khan S.I. (2022). Comparing in vitro Permeability of a Nanocarrier-Hydrogel Hybrid System with an Alcoholic Hydrogel for Sustained Transdermal Drug Delivery. Proceedings of the World Congress on Recent Advances in Nanotechnology.

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Electrochemical Analysis of Cu Plates

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The Cu plate (≥ 99.99%) was purchased from Sigma-Aldrich, USA. Acetonitrile (high-performance liquid chromatography [HPLC] grade, 99.8%) was obtained from Daejung Chemicals & Metals Co., Ltd, South Korea. Propionitrile (> 99.0%) and butyronitrile (> 99.0%) were acquired from TCI, Japan. All materials were used as received.

Begildayeva T., Ahn A., Naik S.S., Lee S.J., Theerthagiri J., Kim T.H, & Choi M.Y. (2021). Facile one-pot synthesis of CuCN by pulsed laser ablation in nitrile solvents and mechanistic studies using quantum chemical calculations. Scientific Reports, 11, 14389.

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