Adenosine 5 triphosphate atp disodium salt hydrate

Manufactured by Merck Group

Sourced in United States

Adenosine 5′-triphosphate (ATP) disodium salt hydrate is a chemical compound commonly used in various scientific and laboratory applications. It serves as a fundamental energy currency in biological systems, playing a crucial role in cellular processes. This product is available in a hydrated form, providing a stable and consistent source of ATP for experimental and research purposes.

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Lab products found in correlation

4 2 hydroxyethyl piperazine 1 ethanesulfonic acid hepes, by Merck Group (3 mentions) Imipramine, by Merck Group (3 mentions) C2 cer, by Merck Group (3 mentions) Lipopolysaccharides (lps), by Merck Group (3 mentions) Verapamil, by Merck Group (3 mentions) Magnesium acetate tetrahydrate, by Merck Group (2 mentions) Ammonium hydroxide, by Merck Group (2 mentions) Ammonium acetate, by Merck Group (2 mentions) Formic acid, by Merck Group (2 mentions) Adenosine 3 5 cyclic monophosphate, by Merck Group (2 mentions) Lysis buffer, by Cell Signaling Technology (2 mentions) Anti txnip, by Abcam (2 mentions) Sulfo n succinimidyl oleate, by Merck Group (2 mentions) Anti caspase 1, by Cell Signaling Technology (2 mentions) Anti p nf κb, by Abcam (2 mentions) Anti cd36, by Abcam (2 mentions) Anti nuclear factor κb nf κb, by Abcam (2 mentions) Anti β actin, by Cell Signaling Technology (2 mentions) Phorbol 12 myristate 13 acetate, by Merck Group (2 mentions) Anti nlrp3, by Abcam (2 mentions)

27 protocols using adenosine 5 triphosphate atp disodium salt hydrate

Mitochondrial Hydrogen Sulfide Donor Synthesis

Cited 1 time

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AP39, a novel mitochondria-targeted H₂S donor, was designed and synthesized by Medicilon Inc., as described previously [19 (link), 20 (link)]. Antimycin A, 0.25% trypsin, 2-deoxyglucose, oligomycin, carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP), trichloroacetic acid, rotenone, 7-azido-4-methylcoumarin (AzMC), monobasic potassium phosphate, dibasic sodium succinate hexahydrate, adenosine 5′-triphosphate (ATP) disodium salt hydrate, fatty acid-free BSA, sodium hydrosulfide hydrate (NaSH·xH₂O), N,N-diethyl-p-phenylenediamine sulfate, magnesium chloride, and iron(III) chloride (FeCl₃) were purchased from Sigma-Aldrich Company. The monoclonal anti-Aβ antibody 6E10 was obtained from Covance Inc. (Princeton, NJ, USA). Drp1 was purchased from Novus Biologicals, Inc. Fis1 was purchased from Proteintech Group. Mfn1 and Mfn2 antibodies were purchased from Santa Cruz Biotech, and OPA-1 antibody was purchased from BD Transduction Laboratories. The secondary antibodies goat anti-mouse-HRP and donkey anti-rabbit-HRP antibodies were purchased from GE Healthcare. The Pierce BCA protein assay kit was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Dulbecco's modified Eagle's medium (DMEM), 1% glutamine, and 1% penicillin were obtained from Invitrogen. All other chemicals were purchased from Amresco (Solon, OH, USA).

Zhao F.L., Fang F., Qiao P.F., Yan N., Gao D, & Yan Y. (2016). AP39, a Mitochondria-Targeted Hydrogen Sulfide Donor, Supports Cellular Bioenergetics and Protects against Alzheimer's Disease by Preserving Mitochondrial Function in APP/PS1 Mice and Neurons. Oxidative Medicine and Cellular Longevity, 2016, 8360738.

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Inhibition of AChE and GSK-3β Activities

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Acetylcholine iodide (ACh),
butyrylthiocholine chloride (BCh), electric eel AChE (EC 3.1.1.7),
horse serum BChE (EC 3.1.1.8), 5,5′-dithiobis[2-nitrobenzoic
acid], quercetin, and berberine were purchased from E. Merck, Fluka,
or Sigma, unless otherwise stated. BACE1 fluorescence resonance energy
transfer (FRET) assay kits (β-secretase) were purchased from
Pan Vera Co. (Madison, WI). Human recombinant GSK-3β was obtained
from ProSpec (ProSpec-Tany TechnoGene Ltd., Ness-Ziona, Israel). The
GSM substrate mimicking muscle glycogen synthase was purchased from
Merck Millipore (Millipore Corporation, CA). Kinase-Glo kits were
obtained from Promega (Promega Corporation, Madison, WI). Adenosine
5-triphosphate (ATP) disodium salt hydrate, ammonium hydroxide, 4-(2-hydroxyethyl)
piperazine-1-ethanesulfonic acid (HEPES), ammonium acetate, formic
acid, ethylene glycol-bis(aminoethylether)-N,N,N,N-tetra acetic acid
tetra sodium salt (EGTA), dimethyl sulfoxide (DMSO), ethylenediaminetetraacetic
acid (EDTA), amyloid-β protein fragments (Aβ_1–40/42), thioflavin T, 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP), and magnesium
acetate tetrahydrate were purchased from Sigma-Aldrich (St. Louis,
MO). All chemicals and solvents from commercial sources were of reagent
grade.

Paudel P., Seong S.H., Zhou Y., Ha M.T., Min B.S., Jung H.A, & Choi J.S. (2019). Arylbenzofurans from the Root Bark of Morus alba as Triple Inhibitors of Cholinesterase, β-Site Amyloid Precursor Protein Cleaving Enzyme 1, and Glycogen Synthase Kinase-3β: Relevance to Alzheimer’s Disease. ACS Omega, 4(4), 6283-6294.

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Quantification of Oxidative Stress Markers

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1,4-Benzoquinone, dimethyl sulfoxide, oligomycin A, adenosine 5’-triphosphate (ATP) disodium salt hydrate, 1-octane-sulfonic acid, sodium phosphate, acetonitrile, glutathione, glutathione disulfide, and diethylenetriaminepentaacetic acid were obtained from Sigma Aldrich (St. Louis, MO, USA).

Doskey C.M., van ‘t Erve T.J., Wagner B.A, & Buettner G.R. (2015). Moles of a Substance per Cell Is a Highly Informative Dosing Metric in Cell Culture. PLoS ONE, 10(7), e0132572.

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Assay for Glycogen Synthase Kinase-3 Activity

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Recombinant glycogen synthase kinase-3 (GSK3) was purchased from New England Biolabs (Hitchin, UK). Lithium chloride (99%, LiCl) and adenosine 5′-triphosphate (ATP) disodium salt hydrate (99%, 5′-ATP-Na₂) were purchased from Sigma-Aldrich (Yongin, Korea). Tetramethyl-6-carboxyrhodamine (TAMRA)-labeled peptides (T-Pep, TAMRA-KEEPPSPPQSPR; T-Pep(p), TAMRA-KEEPPSPPQpSPR; T-Pep(pp), TAMRA-KEEPPpSPPQpSPR, where the underlined letters indicate the phosphorylation site) were synthesized from Peptron, Inc. (Daejeon, Korea). Antibodies to GSK3α/β (#5676) and phospho GSK3α/β (#9327) were from Cell Signaling Technology (Beverly, MA, USA). Horseradish peroxidase-conjugated goat anti-rabbit IgG (ab6721) and Alexa488-conjugated donkey anti-rabbit IgG (ab150061), and rabbit normal IgG (ab172730) were from Abcam (Cambridge, UK). The enhanced chemiluminescence (ECL)-Plus kit was from Amersham-Pharmacia (Buckinghamshire, UK). Protease inhibitor cocktail Xpert was from GenDEPOT (Barker, TX, USA). All other chemicals were of analytical grade and were used as received.

Choi H., Choi B., Seo J.T., Lee K.J., Gye M.C, & Kim Y.P. (2016). Rapid Detection of Glycogen Synthase Kinase-3 Activity in Mouse Sperm Using Fluorescent Gel Shift Electrophoresis. Sensors (Basel, Switzerland), 16(4), 551.

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Antimicrobial Susceptibility Testing Assays

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E. coli ATCC 25922, K. pneumoniae ATCC 13883, A. baumannii ATCC 19606, and P. aeruginosa ATCC 27853 were obtained from the American Type Culture Collection (ATCC). A22 was obtained from Cayman Chemical Company, and CBR-4830 was synthesized as described previously [24 (link)]. Luria–Bertani (LB) media, cation-adjusted Mueller Hinton (CAMH) media, and tryptic soy agar (TSA) were obtained from Becton-Dickson. Ampicillin sodium salt was obtained from VWR, phosphate-buffered saline (PBS) was obtained from Lonza, and adenosine 5’-triphosphate (ATP) disodium salt hydrate was obtained from Sigma-Aldrich (St. Louis, MO, USA).

Bryan E.J., Sagong H.Y., Parhi A.K., Grier M.C., Roberge J.Y., LaVoie E.J, & Pilch D.S. (2022). TXH11106: A Third-Generation MreB Inhibitor with Enhanced Activity against a Broad Range of Gram-Negative Bacterial Pathogens. Antibiotics, 11(5), 693.

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Superparamagnetic Bead-Based Anthrax Toxin Assay

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Superparamagnetic Dynabeads MyOne Tosyl-activated beads were from Life Technologies (Carlsbad, CA). Recombinant EF was produced as previously described [17 (link)]. Activated recombinant PA (PA63) was from List Biological Laboratories (Campbell, CA). Pertussis adenylyl cyclase toxin (CyaA) was from Sigma-Aldrich (St. Louis, MO). Adenosine 5′-triphosphate (ATP) disodium salt hydrate, adenosine-¹³C₁₀,¹⁵N₅ 5′-triphosphate sodium salt solution–labeled ATP (L-ATP), adenosine 3′,5′ cyclic monophosphate, Hepes buffer, CaCl₂, MgCl₂, calmodulin from bovine testes, and all other chemicals and reagents were from Sigma-Aldrich, except where indicated. A Kingfisher 96 magnetic purification system (ThermoFisher Scientific Inc., Waltham, MA) was used for automated sample preparation.

Lins R.C., Boyer A.E., Kuklenyik Z., Woolfitt A.R., Goldstein J., Hoffmaster A.R., Gallegos-Candela M., Leysath C.E., Chen Z., Brumlow J.O., Quinn C.P., Bagarozzi DA J.r., Leppla S.H, & Barr J.R. (2019). Zeptomole per milliliter detection and quantification of edema factor in plasma by LC-MS/MS yields insights into toxemia and the progression of inhalation anthrax. Analytical and bioanalytical chemistry, 411(12), 2493-2509.

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Aptamer-Based Fluorescent Biosensor

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Sodium
chloride, tris hydrochloride, 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic
acid (HEPES), polyethylene glycol sorbitan monolaurate (Tween 20),
magnesium chloride hexahydrate, adenosine, guanosine, adenosine 5′-triphosphate
(ATP) disodium salt hydrate, and tris(2-carboxyethyl)phosphine hydrochloride
solution (TCEP) were purchased from Sigma-Aldrich (Austria). (11-Mercaptoundecyl)triethylene
glycol (PEG-thiol, SPT-0011) was obtained from SensoPath Technologies
(USA). Two DNA aptamers were synthesized by Integrated DNA Technologies
(IDT-DNA, Leuven, Belgium). The ATP hairpin aptamer with the short
loop sequence (SLA) consisted of Alexa Fluor 647N 5′-CA CCT
GGG GGA GTA TTG CGG AGG AAG GTT PEG6 CCA GGT G-SH 3′ and the
long loop ATP hairpin aptamer (LLA) sequence was Alexa Fluor 647N
5′-CA CCT GGG GGA GTA TTG CGG AGG AAG GTT TTT TTT TTT TTT TTT
TTT TTT TTT TTT TTT CCA GGT G TT PEG6 -SH 3′. All reagents
were used as received and buffer solutions were prepared using ultrapure
water (Arium Pro, Sartorius Stedim).

Sergelen K., Fossati S., Turupcu A., Oostenbrink C., Liedberg B., Knoll W, & Dostálek J. (2017). Plasmon Field-Enhanced Fluorescence Energy Transfer for Hairpin Aptamer Assay Readout. ACS Sensors, 2(7), 916-923.

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RVFV Strain Propagation and Titration

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A South African virulent 56/74 RVFV strain (viral stock provided by Alejandro Brun, INIA), isolated from cattle in 1974 [18 (link)] was used. The virus was passaged twice in Aedes albopictus clone C6/36 cells and titrated in African green monkey kidney (Vero) cells (both cell lines provided by Joan Pujols, IRTA-CReSA, Barcelona, Spain) to obtain a 50% tissue culture infective dose per millilitre (TCID₅₀/ml) [19 (link)]. For mosquito blood meals, fresh heparinized bovine blood (Servei de granja i camps experimentals (SGICE), Veterinary Faculty, Autonomous University of Barcelona) was supplemented with adenosine 5’-triphosphate (ATP) disodium salt hydrate (5 × 10⁻³ M) (Sigma-Aldrich, St. Louis, MO, USA) as phagostimulant. Infectious blood meals were prepared by mixing (1:3) bovine blood and virus to obtain a final concentration of 7.5 log₁₀ TCID₅₀/ml. The viral dose employed in our assay was similar to those detected previously in blood samples from experimentally infected European lambs [19 (link)].

Birnberg L., Talavera S., Aranda C., Núñez A.I., Napp S, & Busquets N. (2019). Field-captured Aedes vexans (Meigen, 1830) is a competent vector for Rift Valley fever phlebovirus in Europe. Parasites & Vectors, 12, 484.

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NLRP3 Inflammasome Activation Pathway

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C2-Cer, LPS, adenosine 5′-triphosphate (ATP) disodium salt hydrate, SN50, verapamil, imipramine, sulfo-N-succinimidyl oleate (SSO) and phorbol 12-myristate 13-acetate (PMA) were acquired from Sigma-Aldrich (St Louis, MO, USA). Anti-NLRP3, anti-TXNIP, anti-CD36, anti-nuclear factor-κB (NF-κB) and anti-p-NF-κB antibodies were purchased from Abcam (San Francisco, CA, USA). The secondary antibody was obtained from ZSGB-BIO (Beijing, China). Anti-caspase-1, anti-β-actin, and lysis buffer were obtained from Cell Signaling Technology (Beverly, MA, USA).

Jiang J., Shi Y., Cao J., Lu Y., Sun G, & Yang J. (2021). Role of ASM/Cer/TXNIP signaling module in the NLRP3 inflammasome activation. Lipids in Health and Disease, 20, 19.

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Biochemical Analysis of Ion Transport

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Calcium chloride dihydrate was purchased from G-Biosciences (St Louis, MO, USA). Sodium carbonate decahydrate was bought from Alfa Aesar (Lancashire, UK). Magnesium chloride hexahydrate, adenosine 5’-triphosphate (ATP) disodium salt hydrate, Apyrase from potatoes, Dulbecco’s phosphate buffered saline (PBS) with calcium chloride and magnesium chloride was ordered from Sigma-Aldrich Company Ltd. Ammonium phosphate dibasic was obtained from Fluka (Germany). Optimal cutting temperature (OCT) compound was supplied by VWR Chemicals (Leuven, Belgium). All of the chemicals were used without further purification.

Sui C., Robinson T.E., Williams R.L., Eisenstein N.M, & Grover L.M. (2023). Triggered metabolism of adenosine triphosphate as an explanation for the chemical heterogeneity of heterotopic ossification. Communications Chemistry, 6, 227.

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