Sodium arsenate

Manufactured by Merck Group

Sourced in United States, India

Sodium arsenate is a chemical compound with the formula Na3AsO4. It is a white, crystalline powder that is soluble in water. Sodium arsenate is primarily used as a laboratory reagent and is commonly employed in various scientific and research applications.

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Lab products found in correlation

Ammonium hydroxide, by Merck Group (2 mentions) Sodium nitrate, by Merck Group (2 mentions) Hela cell, by American Type Culture Collection (2 mentions) Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (2 mentions) Urea, by Merck Group (2 mentions) Potassium chloride (kcl), by Merck Group (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Cadmium nitrate, by Merck Group (2 mentions) Hydrochloric acid (hcl), by Merck Group (2 mentions) Lead nitrate, by Merck Group (2 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions) C57bl 6j mice, by Jackson ImmunoResearch (1 mentions) Methacrylamide, by Merck Group (1 mentions) Activated charcoal, by Merck Group (1 mentions) 2 2 azobis 2 methylpropionamidine dihydrochloride, by Merck Group (1 mentions) Hydrochloric acid hcl 12 m, by Thermo Fisher Scientific (1 mentions) Methacrylic acid, by Merck Group (1 mentions) Ethylene glycol dimethacrylate, by Merck Group (1 mentions) Methyl methacrylate, by Merck Group (1 mentions) Lb agar, by BD (1 mentions)

Close spelling variants of this product

Sodium arsenate (Na2HAsO4) Sodium arsenate dibasic heptahydrate (Na2HAsO4.7H2O) Sodium arsenate heptahydrate Sodium arsenate dibasic heptahydrate Sodium arsenate dibasic heptahydrate (Na2HAsO4⋅7H20)

23 protocols using sodium arsenate

Sodium Arsenate Solution Preparation

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The 50-ppm sodium arsenate solution was prepared by dissolving 5 mg of sodium arsenate (Sigma-Aldrich, Saint Louis, MO, USA) in 100 mL of purified water. A fresh solution was prepared every three days for the duration of the study.

Shoaib S.M., Afzal S., Feezan A., Akash M.S., Nadeem A, & Mir T.M. (2023). Metabolomics Analysis and Biochemical Profiling of Arsenic-Induced Metabolic Impairment and Disease Susceptibility. Biomolecules, 13(9), 1424.

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Measuring GAPDH Activity in Cell Variants

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In order to measure the GAPDH activity of the various O and T variants cell pellets were resuspended in 1 ml PBS, disrupted by sonication and clarified at 13,000 × g for 10 min at 4 °C. Extracts were kept on ice until required. The GAPDH assay was a measurement of the reduction of NAD (β-Nicotinamide adenine dinucleotide hydrate) according to Fillinger et al.^{34 (link)}, with modifications. Using a 1 ml disposable cuvette, 900 μl triethanolamine/sodium arsenate buffer (125 mM triethanolamine [Sigma], 5 mM L-cysteine [Sigma], 20 mM sodium arsenate [Sigma], 50 mM disodium hydrogen phosphate, Na₂HPO₄ [Merck], pH 9.2), 2 mM NAD [Sigma] and 60 μl cell extracts were mixed and read at A_340nm at 25 °C. Initially, the mixture was allowed to react for about 10 seconds to ensure that there is nothing in the solution reducing the NAD. Thereafter, 4 mM D-glyceraldehyde-3-phosphate, G-3-P (substrate) [Sigma] was added and the reaction was recorded for 3–5 minutes. One unit causes an initial reaction of reduction of one micromole of NAD per minute and is calculated as follows, where the ΔA₃₄₀/minute is the linear portion of the graph:

Units per mg = \frac{Δ A 340 nm per minute}{6.22 \times mg enzyme per ml reaction mixture}

The amount of GAPDH was then calculated relative to the O variant of each strain. Data were analysed using Student’s unpaired t-test.

Chai M.H., Weiland F., Harvey R.M., Hoffmann P., Ogunniyi A.D, & Paton J.C. (2017). Proteomic comparisons of opaque and transparent variants of Streptococcus pneumoniae by two dimensional-differential gel electrophoresis. Scientific Reports, 7, 2453.

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Arsenic Exposure Impacts Murine Brain Development

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All procedures were conducted in accordance with protocols approved by the Institutional Animal Care and Use Committee at the University of New Mexico. Animals were maintained in a 22 °C vivarium on a 12-h reverse light/dark cycle with lights off at 08:00 and ad libitum access to water and food. Exposure to arsenic during all three trimesters of brain development was performed as previously described (Tyler and Allan, 2013 (link)). Briefly, singly housed female C57BL/6J mice aged 55 days (Jackson Laboratories, Bar Harbor, ME) were acclimated to drinking 50 ppb arsenic-laced water (sodium arsenate, Sigma–Aldrich, St. Louis, MO) for seven to ten days prior to mating. Dams were provided either 50 ppb arsenic water or tap water during breeding and pregnancy until offspring were weaned (Fig. 1A). Offspring were housed two per cage with tap water for the remainder of the study. The number of dams used in this study is reflected in the number of litters provided for each experimental analysis (i.e. n = 8–10 indicates that only one animal was used from each of 8 to 10 litters from 8 to 10 different dams). Adult male offspring (postnatal day 70) from dams drinking arsenic water were euthanized by rapid decapitation for tissue dissection or by overdose with sodium pentobarbital followed by transcardiac perfusion with 4% paraformaldehyde for neurogenesis assessment.

Tyler C.R., Solomon B.R., Ulibarri A.L, & Allan A.M. (2014). Fluoxetine treatment ameliorates depression induced by perinatal arsenic exposure via a neurogenic mechanism. Neurotoxicology, 44, 98-109.

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Synthesis of Arsenic-Removing Polymer Sorbent

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The following materials were obtained and used as received from Sigma Aldrich (St. Louis, MO, USA): methacrylic acid (99%), methacrylamide (98%), methyl methacrylate (98%), 2,2′-Azobis(2-methylpropion-amidine) dihydrochloride (AAPD) (97%), ethylene glycol dimethacrylate (EDMA) (98%), sodium arsenate (98%), ammonium hydroxide (28%), urea (99–100%), and activated charcoal (99.997%, 100 mesh size). Ion exchange resins (Ambersep 21K and Ambersep G-26-H) were obtained from Dupont (Wilmington, DE, USA). Deionized ultra-filtered (DIUF) water was obtained from an E-Pure water purification system (Barnstead E-Pure D4641, Dubuque, IA, USA) and was collected at 18 M Ohm. Hydrochloric acid (HCl) (12 M) was obtained from Fisher Scientific (Waltham, MA, USA).

Kirisenage P.M., Zulqarnain S.M., Myers J.L., Fahlman B.D., Mueller A, & Marquez I. (2022). Development of Adsorptive Membranes for Selective Removal of Contaminants in Water. Polymers, 14(15), 3146.

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Arsenic Tolerance Levels Determination

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The Tolerance levels to arsenic (TLA) for the isolates was determined by the agar dilution technique, on Luria Bertani (LB) agar (Becton Dickinson and Company, Maryland, MO, USA) plates supplemented with different concentrations of sodium arsenite (0.5–100 mM) (Sigma-Aldrich, San Luis, MO, USA) or sodium arsenate (0.5–1000 mM) (Sigma-Aldrich, San Luis, USA). Each plate was inoculated with cell suspensions from fresh pre-cultures at a final density of approximately 10⁷ colony forming units (CFU) mL⁻¹ and incubated for 24 h at 25 °C. To be used as control, LB agar plates were seeded with bacteria but without the metalloid. The criterion to consider bacterial strains as resistant or sensitive to As(III) or (As(V) was the one described by Rokbani et al. [35 (link)]. The bacterial strain capable to tolerate the highest arsenite and arsenate concentrations was selected to perform all the following experiments.

Herrera C., Moraga R., Bustamante B., Vilo C., Aguayo P., Valenzuela C., Smith C.T., Yáñez J., Guzmán-Fierro V., Roeckel M, & Campos V.L. (2021). Characterization of Arsenite-Oxidizing Bacteria Isolated from Arsenic-Rich Sediments, Atacama Desert, Chile. Microorganisms, 9(3), 483.

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Synthesis of Functionalized Nanomaterials

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Potassium phosphate (KH₂PO₄ and K₂HPO₄), potassium chromate (K₂CrO₄), sodium arsenate (Na₂HAsO₄·7H₂O), sodium chloride (NaCl), sodium nitrate (NaNO₃), sodium bicarbonate (NaHCO₃), sodium sulfate (Na₂SO₄), sodium citrate (Na₃C₆H₅O₇·2H₂O), sodium acetate (NaC₂H₃O₂), potassium chloride (KCl), dimethoxyethane (glyme), [3-(2-aminoethylamino)propyl] trimethoxysilane, 25-kDa branched polyethyleneimine (PEI), Cab-o-sil® M5 and StratoSpheres™ PL-EDA polystyrene resin were purchased from Sigma Aldrich (St. Louis, MO). Ferric chloride (FeCl₃·6H₂O), acetonitrile (CH₃CN), methanol, 2-propanol, dimethyl sulfoxide (DMSO), sodium hydroxide (NaOH), hydrochloric acid (HCl) and nitric acid (HNO₃) were purchased from Fisher Scientific (Waltham, MA). Ultrapure SiliaBond® Diamine functionalized silica gel was purchased from Silicycle® (Quebec City, QC). 3-isocyanatopropyl trimethoxysilane was purchased from Gelest (Morrisville, PA). Sevelamer HCl was purchased from Genzyme (Cambridge, MA) and AG® 1-X8 anion exchanger was purchased from BioRad (Hercules, CA). All common chemicals and reagents were of high purity grade.

Sangvanich T., Ngamcherdtrakul W., Lee R., Morry J., Castro D., Fryxell G.E, & Yantasee W. (2014). Nanoporous sorbent material as an oral phosphate binder and for aqueous phosphate, chromate, and arsenate removal. Journal of nanomedicine & nanotechnology, 5, 1000222.

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Polymer-Magnetite Nanocomposites for Arsenic and Heavy Metal Removal

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All chemicals used in this
study were of analytical grade. For the preparation of polymer suspensions,
polyacrylonitrile (PAN, Sigma Aldrich) with a molecular weight of
10201 g/mol and dimethylformamide (99.5%, DMF, Sigma Aldrich) were
used. Magnetite nanoparticles were synthesized using ammonium hydroxide
(25%, Sigma Aldrich), ammonium ferrous sulfate (≥98%, Sigma
Aldrich), and ammonium ferric sulfate (99.0%, Fischer). Titanium dioxide
(TiO₂) (anatase, 99.7%) was purchased from Sigma Aldrich.
For the adsorption studies, sodium arsenate (Na₂HAsO₄·7H₂O, ≥98%, Sigma Aldrich), cadmium
nitrate tetrahydrate (Cd(NO₃)₂·4H₂O, 99%, Riedel-de-Haen), calcium nitrate tetrahydrate (Ca(NO₃)₂·4H₂O, 99%, Sri Chem), and magnesium
nitrate hexahydrate (Mg(NO₃)₂·6H₂O, 99%, Sri Chem) were used, and the aqueous solutions were prepared
with ASTM Type I water obtained from Industrial Technology Institute,
Sri Lanka.

Siriwardane I.W., Sandaruwan C., de Silva R.M., Williams G.R., Gurgul S.J., Dziemidowicz K, & de Silva K.M. (2021). Nanomagnetite- and Nanotitania-Incorporated Polyacrylonitrile Nanofibers for Simultaneous Cd(II)- and As(V)-Ion Removal Applications. ACS Omega, 6(42), 28171-28181.

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Sodium Arsenate and DRLC Protocol

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Sodium arsenate was procured from Sigma Company (St. Louis, MO, USA) and D-Ribose-L-Cysteine (DRLC) was procured from Max International, Salt Lake, USA. All other chemicals used in the study were of analytical reagent grade.

Ogunlade B., Adelakun S.A., Ukwenya V.O, & Elemoso T.T. (2021). Potentiating response of D- Ribose-L-Cysteine on Sodium arsenate- induced hormonal imbalance, spermatogenesis impairments and histomorphometric alterations in adult male Wistar rat. JBRA Assisted Reproduction, 25(3), 358-367.

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Rearing and Culturing Ae. aegypti and E. coli

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Larval Ae. aegypti were reared at 26 °C on a diet of brewer’s yeast and lactalbumin (1:1) under 16:18 h light:dark cycle. Adults were fed on 10% sucrose or on chicken blood. Females were used 3–5 days after emergence.
E. coli CGSC strain7636: F⁻D(araD-araB)567, DlacZ4787(::rrnB-3), l⁻, rph-1,D(rhaD-rhaB)568, hsdR514 and E. coli CGSC strain 8547:
F⁻D(araD-araB)567, DlacZ4787(::rrnB-3), DsbmA742::kan, l⁻, rph-1,D(rhaD-rhaB)568, hsdR514 (http://cgsc.biology.yale.edu/StrainRpt.php) were grown in Luria-Bertani (LB) at 37 °C under aerobic conditions with the addition, when required, of the appropriate antibiotics at the following concentrations 100 μg/mL for ampicillin and 50 μg/mL for kanamycin. Valinomycin, 2,4-dinirophenol, sodium azide and sodium arsenate were purchased from Sigma-Aldrich and diluted in M9 medium.

Borovsky D., Deckers K., Vanhove A.C., Verstraete M., Rougé P., Shatters RG J.r, & Powell C.A. (2021). Cloning and Characterization of Aedes aegypti Trypsin Modulating Oostatic Factor (TMOF) Gut Receptor. Biomolecules, 11(7), 934.

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Detailed Antibody and Reagent Specifications

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The primary antibodies used in this study were specific for G3BP1 (Abcam; catalog no.: b181150), G3BP1 (Proteintech; catalog no.: 13057-2-AP), TIAR (Cell Signaling Technology; catalog no.: 8509S), GFP (Abcam; catalog no.: ab290), FLAG (Cell Signaling Technology; catalog no.: 14793S), HA (Cell Signaling Technology; catalog no.: 3724S), GAPDH (Proteintech; catalog no.: 10494-1-AP), ASFV p30, p72 (prepared in our laboratory), and pS273R (gifted by Professor Jianzhong Zhu). IRDye 800CW goat antimouse immunoglobulin G (IgG) (H + L) (catalog no.: 926-32210) was purchased from Sera Care, and IRDye 800CW goat anti-rabbit IgG (H + L) (catalog no.: 925-32211) was purchased from LI-COR. Alexa Flour 488 goat anti-rabbit IgG (H + L) and Alexa Flour 594 goat antimouse IgG (H + L) were all purchased from Invitrogen. The protein agarose A/G used for co-IP was purchased from Santa Cruz Biotechnology (catalog no.: 20397). Sodium arsenate (Sigma–Aldrich; catalog no.: S7400) was dissolved in water at the storage concentration of 500 mM.

Li T., Li X., Wang X., Chen X., Zhao G., Liu C., Bao M., Song J., Li J., Huang L., Rong J., Tian K., Deng J., Zhu J., Cai X., Bu Z., Zheng J, & Weng C. (2023). African swine fever virus pS273R antagonizes stress granule formation by cleaving the nucleating protein G3BP1 to facilitate viral replication. The Journal of Biological Chemistry, 299(7), 104844.

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