Uninfected and A. phagocytophilum NCH-1 strain–infected human promyelocytic HL-60 cells (CCL-240; American Type Culture Collections [ATCC]) and RF/6A rhesus monkey choroidal endothelial cells (CRL-1780; ATCC) were cultured as previously described (Huang et al, 2010a (link)). HeLa human cervical epithelial cells (CCL-2; ATCC) were maintained as described (Justis et al, 2017 (link)). C. burnetii Nine Mile Phase II (NMII; clone 4, RSA439) and mCherry-C. burnetii NMII were purified from Vero cells (African green monkey kidney epithelial cells; CCL-81; ATCC) or acidified citrate cysteine medium-2 (ACCM-2) and stored as described (Cockrell et al, 2008 (link); Beare et al, 2009 (link)). Mouse alveolar macrophages (MH-S; CRL-2019; ATCC) and THP-1 human monocytic cells (TIB-202; ATCC) were maintained as described (Mulye et al, 2018 (link)). THP-1 cells were differentiated into macrophage-like cells by overnight treatment with 200 nM phorbol 12-myrisate 13-acetate (MilliporeSigma). C. trachomatis serovar L2 (LGV 434) was maintained in HeLa cells at 37°C as described (Rucks et al, 2017 (link)). C. pneumoniae AR39 was maintained in HeLa cells at 35°C as described (Ouellette et al, 2016 (link)). Mammalian cell cultures were low passage and confirmed to be mycoplasma free using the Universal Mycoplasma Detection kit (ATCC) or Mycoplasma PCR Detection kit (MilliporeSigma).
Crl 2019
Manufactured by American Type Culture Collection
Sourced in United States
The CRL-2019 is a laboratory equipment product offered by the American Type Culture Collection. It is designed for cell culture applications, but a detailed description of its core function cannot be provided in a concise, unbiased, and factual manner without potential for extrapolation. Therefore, the description for this product is not available.
Automatically generated - may contain errors
Lab products found in correlation
Ccl 81, by American Type Culture Collection (1 mentions)
Ccl 240, by American Type Culture Collection (1 mentions)
Tib 202, by American Type Culture Collection (1 mentions)
Universal mycoplasma detection kit, by American Type Culture Collection (1 mentions)
Crl 1780, by American Type Culture Collection (1 mentions)
F12 media, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by GE Healthcare (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions)
Ccl 185, by American Type Culture Collection (1 mentions)
Cytation 5 multimode imager, by Agilent Technologies (1 mentions)
Celltiter glo 2.0 cell viability assay, by Promega (1 mentions)
Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions)
Rpmi 1640 medium, by Corning (1 mentions)
Tib 67, by American Type Culture Collection (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Crl 1611, by American Type Culture Collection (1 mentions)
Penicillin streptomycin mixed solution, by Nacalai Tesque (1 mentions)
6 protocols using crl 2019
1
Culturing Host Cells for Anaplasma, Coxiella, and Chlamydia Infections
2
Cytotoxicity Evaluation of UiO-66 Nanoparticles
3
Infection Dynamics of C. burnetii Strains
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C. burnetii Nine Mile phase II (NMII clone 4, RSA 439) wild type (WT) and ΔdotA mutant [69 (link)] were grown for 4 days in acidified citrate cysteine medium 2 (ACCM-2) at 37°C in 2.5% O2 and 5% CO2, washed twice with phosphate-buffered saline (PBS), and stored as previously described [70 (link)]. Murine alveolar (MH-S) macrophages (CRL-2019; ATCC) were maintained in growth medium consisting of RPMI 1640 medium (Corning) containing 10% fetal bovine serum (FBS; Atlanta Biologicals) at 37°C in 5% CO2. The multiplicity of infection (MOI) was optimized for each bacterial stock and culture vessel for a final infection of approximately 1 internalized bacterium per cell.
4
Culturing Lung Cells and Macrophages
5
Murine Macrophage Cell Culture Protocol
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Murine macrophage-like J774A.1 (ATCC® TIB67™) and murine alveolar macrophages MHS (ATCC® CRL-2019™) were maintained at 37 °C and 5% CO2 in RPMI-1640 medium [L-Glutamine]. For J774 cells, the medium was supplemented with 10% heat-inactivated Foetal Bovine Serum (FBS) and 100 U/ml Penicillin/Streptomycin. For MHS, the medium was supplemented with 10% FBS and 2-mercaptoethanol at a final concentration of 0.05 mM. Cells were subcultured every 2-3 days and used at passage numbers between 12 and 20. Unless otherwise mentioned, MHS and J774 cells were seeded in 24-well plates at a cell density of 10 5 cells/well and incubated overnight at 37 °C and 5% CO2 before exposure to test compounds or controls.
6
Glycolipid Analysis in Kidney Cell Lines
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Cells were cultured as previously described [59 (link)]. ACHN (CRL-1611, ATCC, Manassas, VA, USA) cells and human kidney proximal tubule cell line (HK-2, CRL-2019, ATCC) were grown in Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FBS and 1% penicillin–streptomycin mixed solution (Nacalai Tesque, Kyoto, Japan). Each cell line was seeded at 1 × 1,000,000 cells/dish for GSL analysis. After seeding for 2 days and collecting the medium, 1 mL of the cells were washed twice with 2 mL ice-cold PBS and subsequently collected from the Petri dish using a scraper. Finally, the suspended cells were counted and analyzed using LC–MS/MS. The cell extract (1 mL) in PBS or medium was added to the pretreatment method described above. The supernatant was dried, redissolved in 100 µL of 75% ethanol, and 50 µL was used for GSL analysis.
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