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EF-1α is a cellular protein that plays a crucial role in protein synthesis. It functions as a GTPase and is responsible for delivering aminoacyl-tRNA to the ribosome during translation.

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Lab products found in correlation

Gapdh, by Merck Group (1 mentions) Ab96621, by Abcam (1 mentions) β catenin, by Cell Signaling Technology (1 mentions) Keygen nuclear and cytoplasmic protein extraction kit, by Keygen Biotech (1 mentions) Gapdh, by Abcam (1 mentions) Hsc70, by Novus Biologicals (1 mentions) Lamp2a, by Thermo Fisher Scientific (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Hsp90, by Cell Signaling Technology (1 mentions)

2 protocols using ef 1α

1

Analyzing Wnt Signaling Pathway Proteins

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Total proteins were extracted using RIPA buffer (50 mM Tris (pH 7.4), 1 mM EDTA, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate) supplemental with protease inhibitors (Roche). Western blot assay was performed as previously described28 (link) with antibodies against DDX39 (ab96621, Abcam), TCF4 (#2596, Cell Signaling Technology), LEF1 (#2230, Cell Signaling Technology) and GAPDH (G8795, Sigma). For nuclear proteins extraction, KeyGEN Nuclear and Cytoplasmic Protein Extraction Kit (KGP150, KeyGEN BioTECH) was used, the antibodies against β-catenin (#8480, Cell Signaling Technology) and EF-1α (#2551, Cell Signaling Technology) were used.
Zhang T., Ma Z., Liu L., Sun J., Tang H., Zhang B., Zou Y, & Li H. (2018). DDX39 promotes hepatocellular carcinoma growth and metastasis through activating Wnt/β-catenin pathway. Cell Death & Disease, 9(6), 675.
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2

Antibody Sources for Cellular Analysis

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The sources of antibodies used in this study are LAMP-2A (Invitrogen); GAPDH (Abcam); RNase A (Rockland Immunochemicals); HSP90, β-actin, LC3B, and EF-1α (Cell Signaling Technology); hsc70 (Novus Biological); rHN (generated with residues 20–35 of the rHN peptide and no cross-reaction with HN and HNG; GeneTex); and mTOR CST and GFP antibody (Thermo Fisher Scientific). Antibody against HN or HNG was generated by Genscript using residues 7–24 of the HN peptide.
Gong Z., Tasset I., Diaz A., Anguiano J., Tas E., Cui L., Kuliawat R., Liu H., Kühn B., Cuervo A.M, & Muzumdar R. (2018). Humanin is an endogenous activator of chaperone-mediated autophagy. The Journal of Cell Biology, 217(2), 635-647.
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