B6 cg tg cdh5 cre 7mlia j
Manufactured by Jackson ImmunoResearch
Sourced in United States
The B6.Cg-Tg(Cdh5-cre)7Mlia/J is a genetically modified mouse strain. It expresses Cre recombinase under the control of the Cdh5 promoter, which targets expression to vascular endothelial cells.
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6 protocols using b6 cg tg cdh5 cre 7mlia j
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Genetically engineered mouse models
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Endothelial ADAM17 Knockout Mice
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All the animal procedures were performed in accordance with the statement of the Association for Research in Vision and Ophthalmology (ARVO) for the humane use of animals in vision science and with protocols approved by Augusta University (#2009-0181; approved February 22, 2018). C57Bl/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA). Endothelial-specific ADAM17 knockout (k/o) mice were generated by crossing Adam17tm1.2Bbl/J mice (Stock No: 009597; Jackson Laboratories) which harbor loxP sites flanking exon 2 of ADAM17 with mice expressing Cre recombinase under the control of a Cadh5 promoter (Stock No: 006137; B6.Cg-Tg(Cdh5-cre)7Mlia/J; Jackson Laboratories), as we described before [35 (link)]. When bred with ADAM17flox mice, Cre-mediated recombination results in deletion of ADAM17 in the endothelium of developing and quiescent vessels, as well as within a subset of hematopoietic cells [53 (link)]. Genotypes of mice were determined by PCR using tail genomic DNA and KAPA Mouse Genotyping Kit (KAPA Biosystem, Wilmington, MA, USA).
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Genetically engineered mouse models
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Endothelial Podocalyxin Knockout Mice
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All experiments and procedures were performed in accordance with the requirements of the Canadian Council on Animal Care (CCAC) and the UBC Animal Care Committee approved all animal experimental protocols (protocol #A06-1483). Mice were maintained in a specific pathogen-free facility at the Biomedical Research Centre (Vancouver, BC, Canada). Cdh5-Cre mice (B6.Cg-Tg(Cdh5-cre)7Mlia/J) were purchased from the Jackson Laboratory (Bar Harbor, ME). Conditional podocalyxin knockout mice were generated and crossed with Cdh5-Cre mice to generate mice carrying endothelial-specific deletion of podocalyxin. Male and female mice were used for all experiments unless otherwise stated.
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Genetic Mouse Model for Atherosclerosis Research
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The University of Massachusetts Medical School Institutional Animal Care and Use Committee or the Pfizer Institutional Animal Care and Use Committees approved all of the animal procedures. MAP4K4-pSico mice41 (link) were first crossed with VE-cadherin Cre transgenic mice (B6.Cg-Tg(Cdh5-cre)7Mlia/J, Jackson Laboratories), and these animals were then crossed onto the Apoe−/− background (B6.129P2-Apoetm1Unc/J, Jackson Laboratories). Map4k4 flox/flox animals were crossed with a tamoxifen-inducible endothelial-specific cre mouse line (Cdh5(PAC)-CreERT2) that was obtained from Dr Ralf Adams22 (link), and these animals were crossed onto the ApoE−/− background as above.
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Generation of SENP1 Vascular EC Knockout Mice
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Mice were kept in the animal facilities at Zhejiang University with a 12 h to 12 h light cycle and a humidity- and temperature-controlled environment. All animal experimental protocols were approved by the Institutional Animal Care and Use Committee of Zhejiang University. SENP1 vascular EC knockout mice (SENP1-ecKO) were generated by mating SENP1 flox (SENP1 flox/flox) mice that were created in our previous study31 (link) with vascular EC-specific VE-cadherin Cre transgenic mice (B6.Cg-Tg(Cdh5-cre)7Mlia/J; Jackson Laboratories, ME, USA). Both WT and SENP1-ecKO mice were backcrossed onto the C57BL/6 (B6) background, and they were viable and fertile.
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