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Recombinant mouse il 12

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Recombinant mouse IL-12 is a protein produced in a laboratory setting. It is a cytokine that plays a role in the immune response.

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Lab products found in correlation

Il 18, by R&D Systems (5 mentions) Phorbol myristate acetate (pma), by Merck Group (4 mentions) Ionomycin, by Merck Group (4 mentions) Anti cd28, by BioLegend (3 mentions) Recombinant human il 2, by R&D Systems (2 mentions) Anti cd3 ab, by BioLegend (2 mentions) Anti cd3, by BioLegend (2 mentions) Recombinant human il 2, by Novartis (1 mentions) Dx5 labeled macs beads, by Miltenyi Biotec (1 mentions) Lps from escherichia coli, by Merck Group (1 mentions) Anti cd3 ab, by BD (1 mentions) Soluble anti cd28 ab, by BD (1 mentions) Anti ifn γ, by BD (1 mentions) Human il 2, by BD (1 mentions) Human tgf β1, by Thermo Fisher Scientific (1 mentions) Macs cell sorting system, by Miltenyi Biotec (1 mentions) Anti il 4, by BD (1 mentions) Mouse ifn γ, by R&D Systems (1 mentions) Mouse il 6, by R&D Systems (1 mentions) 1 2 dipalmitoyl sn glycero 3 phosphocholine dppc, by Avanti Polar Lipids (1 mentions)

Close spelling variants of this product

IL-12 (213 citations) Mouse IL-12 (11 citations) Recombinant IL-12 (7 citations)

24 protocols using recombinant mouse il 12

1

Isolation and Expansion of NK Cells

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Spleens were isolated and single cell suspensions were prepared by pressing through 100 μm cell strainers. NK cells were isolated using DX5-labeled MACS® beads according to the manufacturer's protocol (Miltenyi Biotec) and expanded in RPMI1640 complete medium supplemented with 5,000 U/ mL recombinant human IL-2 (Proleukin®, Novartis) for 5–7 d. Purity of NK cells was assessed by flow cytometry and were routinely 85–95% CD3ϵ−NK1.1+ of total cells. Cultivated NK cells were further stimulated with 5 ng/ mL recombinant mouse IL-12 (R&D Systems) for the times indicated and cells were used for Western blot analysis and supernatants for ELISA. For the analysis of the expression of G zmB, Prf1 and miRNAs, cells were deprived from IL-2 for 4 h prior to cell lysis.
Prchal-Murphy M., Witalisz-Siepracka A., Bednarik K.T., Putz E.M., Gotthardt D., Meissl K., Sexl V., Müller M, & Strobl B. (2015). In vivo tumor surveillance by NK cells requires TYK2 but not TYK2 kinase activity. Oncoimmunology, 4(11), e1047579.
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2

In Vitro Polarization of TH1 Cells

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HA-specific CD4+ T cells were sorted, activated in vitro with plate-bound α-CD3 and soluble α-CD28 antibodies (both BD Bioscience), and were polarized into IFN-γ producing TH1 cells by adding recombinant mouse IL-12 (20 ng/μL; R&D Systems, Wiesbaden, Germany) and α-IL-4 antibody (200 ng/μL; eBioscience, San Diego, CA) to the media. At day 6, TH1 polarization was confirmed by flow cytometric staining for IFN-γ.
Frede A., Neuhaus B., Knuschke T., Wadwa M., Kollenda S., Klopfleisch R., Hansen W., Buer J., Bruder D., Epple M, & Westendorf A.M. (2017). Local delivery of siRNA-loaded calcium phosphate nanoparticles abates pulmonary inflammation. Nanomedicine, 13(8), 2395-2403.
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3

Endotoxin-Induced Inflammation in Mice

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Male 8-week-old C57BL6/J mice were purchased from SLC (Shizuoka, Japan) for use in this study. All animal care and experimental procedures were performed in accordance with the Guidelines for Animal Experimentation of Nagasaki University with approval of the Institutional Animal Care and Use Committee. LPS from Escherichia coli was purchased from Sigma (St. Louis, MO). Recombinant mouse IL-12 was purchased from R&D Systems (Minneapolis, MN).
Yoshimatsu M., Kitaura H., Fujimura Y., Kohara H., Morita Y, & Yoshida N. (2015). IL-12 Inhibits Lipopolysaccharide Stimulated Osteoclastogenesis in Mice. Journal of Immunology Research, 2015, 214878.
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4

Liver Lymphocyte Activation Assay

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Approximately 3 × 105 liver lymphocytes were stimulated for 5 hr in RPMI containing 10% fetal bovine serum with either recombinant mouse IL-12 (20 ng/ml; R&D Systems) plus IL-18 (10 ng/ml; R&D Systems), platebound αNK1.1 (PK136) or plate bound αNKp46 (29A1.4). Cells were cultured in media alone as a negative control.
Weizman O.E., Song E., Adams N.M., Hildreth A.D., Riggan L., Krishna C., Aguilar O.A., Leslie C.S., Carlyle J.R., Sun J.C, & O’Sullivan T.E. (2019). Mouse cytomegalovirus-experienced ILC1s acquire a memory response dependent on the viral glycoprotein m12. Nature immunology, 20(8), 1004-1011.
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5

CD4+ T Cell Activation Assay

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CD4+ Tn isolated from WT B6 and T-KO mice were stimulated with anti-CD3 (2μg/ml, Biolegend) and anti-CD28 (2μg/ml, Biolegend) Abs together with recombinant human IL-2 (10ng/ml, R&D Systems) and recombinant mouse IL-12 (5ng/ml, R&D Systems). On Day 7, cells were restimulated in 96-well plates with plate-bound anti CD3 Abs (1μg/ml, Biolegend) for 5 hours before collecting the supernatants. Each group contained equal number of cells (1×106 cells/ml). The concentration of IFN-γ and IL-4 were measured in triplicate by using recombinant mouse IFN-γ and IL-4 ELISA kits in accordance with the manufacturer's instruction (Biolegend).
Tong Q., He S., Xie F., Mochizuki K., Liu Y., Mochizuki I., Meng L., Sun H., Zhang Y., Guo Y., Hexner E, & Zhang Y. (2014). Ezh2 regulates transcriptional and post-translational expression of T-bet and promotes Th1 cell responses mediating aplastic anemia in mice. Journal of immunology (Baltimore, Md. : 1950), 192(11), 5012-5022.
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6

IL-12 Stimulation of NK Cells

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Purified NK cells (WT or Dectin-1−/−; 5 × 104 cells) were stimulated with 10 or 100 ng/ml of recombinant mouse IL12 (R&D Systems, Minneapolis, MN). IFN-γ production in culture supernatants was evaluated by ELISA kit (R&D Systems) after 24-hr incubation.
Chiba S., Ikushima H., Ueki H., Yanai H., Kimura Y., Hangai S., Nishio J., Negishi H., Tamura T., Saijo S., Iwakura Y, & Taniguchi T. (2014). Recognition of tumor cells by Dectin-1 orchestrates innate immune cells for anti-tumor responses. eLife, 3, e04177.
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7

Liver Lymphocyte Stimulation Assay

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Approximately 3 × 105 liver lymphocytes were stimulated for 5 hr in RPMI containing 10% fetal bovine serum with recombinant mouse IL-12 (20 ng/ml; R&D Systems) plus IL-18 (10 ng/ml; R&D Systems). Cells were cultured in media alone as a negative control.
Weizman O.E., Adams N.M., Schuster I., Krishna C., Pritykin Y., Lau C., Degli-Esposti M.A., Leslie C.S., Sun J.C, & O’Sullivan T.E. (2017). ILC1 confer early host protection at initial sites of viral infection. Cell, 171(4), 795-808.e12.
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8

Liver Lymphocyte Activation Assay

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Approximately 3 × 105 liver lymphocytes were stimulated for 5 hr in RPMI containing 10% fetal bovine serum with either recombinant mouse IL-12 (20 ng/ml; R&D Systems) plus IL-18 (10 ng/ml; R&D Systems), platebound αNK1.1 (PK136) or plate bound αNKp46 (29A1.4). Cells were cultured in media alone as a negative control.
Weizman O.E., Song E., Adams N.M., Hildreth A.D., Riggan L., Krishna C., Aguilar O.A., Leslie C.S., Carlyle J.R., Sun J.C, & O’Sullivan T.E. (2019). Mouse cytomegalovirus-experienced ILC1s acquire a memory response dependent on the viral glycoprotein m12. Nature immunology, 20(8), 1004-1011.
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9

Differential T Cell Polarization

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Naïve CD4+ CD62L+ T cells from the spleens of unprimed OT-II TCR transgenic mice were purified using the MACS cell sorting system (Miltenyi Biotec). CD4+ CD62L+ T cells (7.0×105) were stimulated with 5 µg/mL plate-bound anti-CD3 Abs (BD Pharmingen) and 1 µg/ml soluble anti-CD28 Abs (BD Pharmingen) for three days in RPMI-1640 supplemented with 10% fetal bovine serum and 2-ME in the presence of recombinant cytokines. T cells were polarized with 10 ng/mL recombinant mouse IL-12 (R&D Systems), 5 ng/ml mouse IFN-γ (R&D Systems), 20 U/mL human IL-2, and 5 µg/mL anti-IL-4 (BD Pharmingen) for Th1 cell differentiation, 5 ng/mL human TGF-β1 (PeproTech), 20 ng/mL mouse IL-6 (R&D Systems), 20 U/mL human IL-2, and anti-IL-4 and anti- IFN-γ (2 µg/mL; BD Pharmingen) for Th17 cell differentiation, as well as 5.0 ng/mL human TGF-β1 (PeproTech), 100 U/mL human IL-2, anti-IL-4, and anti- IFN-γ (5 µg/mL; BD Pharmingen) for induced Treg (iTreg) cell differentiation.
Ikeda T., Hirata S., Takamatsu K., Haruta M., Tsukamoto H., Ito T., Uchino M., Ando Y., Nagafuchi S., Nishimura Y, & Senju S. (2014). Suppression of Th1-Mediated Autoimmunity by Embryonic Stem Cell-Derived Dendritic Cells. PLoS ONE, 9(12), e115198.
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10

MPL-Lipid Formulation for Tumor Immunotherapy

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MPL from Salmonella enteric serotype Minnesota
RE 595 and cholesterol (Sigma grade ≥99%) were purchased from
Sigma-Aldrich (St. Louis, MO, USA). 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dioleoyl-3-trimethylammonium-propane
(DOTAP) chloride salt were obtained from Avanti Polar Lipids, Inc.
(Alabaster, AL, USA). Mouse Novex Cytokine Magnetic 10-Plex ELISA
kits were purchased from Invitrogen (Grand Rapids, NY, USA). 4T1-luc2-td
Tomato Bioware Ultra Red mouse mammary cancer cells were purchased
from Caliper Life Sciences (Hopkinton, MA, USA). Recombinant mouse
IL-12 was purchased from R&D Systems, Inc. (Minneapolis, MN, USA).
Meraz I.M., Savage D.J., Segura-Ibarra V., Li J., Rhudy J., Gu J, & Serda R.E. (2014). Adjuvant Cationic Liposomes Presenting MPL and IL-12 Induce Cell Death, Suppress Tumor Growth, and Alter the Cellular Phenotype of Tumors in a Murine Model of Breast Cancer. Molecular Pharmaceutics, 11(10), 3484-3491.
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