Serum (for total cholesterol, LDL, HDL, and triglycerides) and plasma (for all other measures) were isolated from cord blood by centrifugation as soon as possible after collection. Samples were stored at -80°C until analysis. The following biochemical measures were obtained: plasma glucose levels (hexokinase method, Sigma); adiponectin (Linco Human Adiponectin ELISA kit, Millipore); leptin (Linco Human Leptin ELISA kit, Millipore); C-peptide ( Human C-peptide Radioimmunoassay, Millipore); insulin (fluoroimmunoassay, Beckman Coulter); Non-esterified fatty acid (NEFA) levels (enzymatic colorimetric method assay, Wako); total cholesterol, LDL cholesterol, HDL cholesterol, and triglyceride levels (Beckman Coulter Unicel® DxC 800 system, timed endpoint methods).
Linco human leptin elisa kit
Manufactured by Merck Group
The Linco Human Leptin ELISA kit is a laboratory equipment product manufactured by Merck Group. It is an enzyme-linked immunosorbent assay (ELISA) designed for the quantitative determination of leptin levels in human serum, plasma, and other biological fluids.
Automatically generated - may contain errors
2 protocols using linco human leptin elisa kit
1
Cord Blood Metabolic Biomarkers
2
Cord Blood Metabolic Biomarkers
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