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Ccr6 bv785

Manufactured by Thermo Fisher Scientific

The CCR6 BV785 is a fluorochrome-conjugated monoclonal antibody designed for the detection and quantification of the CCR6 receptor on the surface of cells using flow cytometry. CCR6 is a chemokine receptor that plays a role in the recruitment of immune cells to sites of inflammation. The BV785 fluorochrome provides a bright signal for improved sensitivity in flow cytometry applications.

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3 protocols using ccr6 bv785

1

Flow Cytometry Immunophenotyping Panel

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Flow cytometry was performed using the following directly conjugated antibodies: (1) BD Biosciences: CD3 H7APC (SK7), CD45RA Cy7PE (L48), CD20 APC-H7 (2H7), CD45RO TRPE (UCHL), CTLA-4 APC (BNI3), IgD PE (IA6-2), IgG APC (G18-145), IgM PE-Cy5 (G20-127), IL-21 Alexa 647 (3A3-N2.1), and Ki-67 Alexa700 (150503); (2) Beckman Coulter: CD127 Cy5PE (R34.34); (3) BioLegend: PD-1 BV711 (EH12.2H7), IFNg BV421 (4S.B3), TNF-α CY7PE (MAb-11), IL-2 Alexa-700 (MQ1-17H12), CCR6 BV785 (G034E3), CD27 Alexa594 (IA4CD27), CD27 BV605 (O323), CCR7-BV605 (G043H7), CD8 BV785 (SK1), ICOS Pac Blue (C398.4A), CD19 Pac Blue (HIB19), CD38 BV785 (HIT2), and CD154 PE (24–31); (4) Invitrogen: CD4 Cy5.5PE (S3.5) and Aqua LIVE/DEAD® amine viability dye; and (5) eBioscience: CXCR5-FITC (MU5UBEE).
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2

Comprehensive Immunophenotyping by Flow Cytometry

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Flow cytometry was performed using the following directly conjugated antibodies: (1) BD Biosciences: CD3 H7APC (SK7), CD45RA Cy7PE (L48), CD20 APC-H7 (2H7), CD45RO TRPE (UCHL), CTLA-4 APC (BNI3), IgD PE (IA6-2), IgG APC (G18-145), IgM PE-Cy5 (G20-127), IL-21 Alexa 647 (3A3-N2.1), and Ki-67 Alexa700 (150503); (2) Beckman Coulter: CD127 Cy5PE (R34.34); (3) BioLegend: PD-1 BV711 (EH12.2H7), IFNg BV421 (4S.B3), TNF-α CY7PE (MAb-11), IL-2 Alexa-700 (MQ1-17H12), CCR6 BV785 (G034E3), CD27 Alexa594 (IA4CD27), CD27 BV605 (O323), CCR7-BV605 (G043H7), CD8 BV785 (SK1), ICOS Pac Blue (C398.4A), CD19 Pac Blue (HIB19), CD38 BV785 (HIT2), and CD154 PE (24-31); (4) Invitrogen: CD4 Cy5.5PE (S3.5) and Aqua LIVE/DEAD® amine viability dye; and (5) eBioscience: CXCR5-FITC (MU5UBEE).
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3

Flow Cytometry Immunophenotyping Panel

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Flow cytometry was performed using the following directly conjugated antibodies (clones): (1) BD Biosciences: CD3 H7APC (SK7 clone), CD45RA PE- Cy7 (L48), (2) BioLegend: CD3 BV650 (OKT3), IgM BV570 (MHM-88), PD-1 BV711 (EH12.2H7), CCR6 BV785 (G034E3), CD27 Alexa Fluor 594 (IA4CD27), CD27 BV605 (O323), CCR7 BV605 (G043H7), CCR7 APC (G043H7), CD8 BV650 (RPA-T8), ICOS Pac Blue (C398.4A), CD38 BV785 (HIT2); CXCR5 Alexa Fluor 488 (J252D4), CXCR3 PE (G025H7) (3) Invitrogen: CD4 PE-Cy5.5 (S3.5) and Aqua LIVE/DEAD® amine viability dye; (4) eBioscience: CXCR5 PE-CY7 (MU5UBEE); (5) Southern Biotech: IgD-PE; (6) Beckman Coulter: CD19 ECD (J3-119). The IgG (Alexa Fluor 680) and CD21 (PE-CY5.5) were conjugated in-house.
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