Ivos 2 system

Manufactured by Hamilton Thorne

Sourced in United States

The IVOS II™ system is a computer-assisted sperm analysis (CASA) instrument designed for the assessment and evaluation of semen samples. The system utilizes advanced imaging technology and algorithms to objectively measure and analyze various sperm parameters, including concentration, motility, and morphology. The IVOS II™ system provides a comprehensive and standardized assessment of sperm quality to support various applications in the field of assisted reproductive technology and research.

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Lab products found in correlation

Htf medium, by Irvine Scientific (1 mentions)

4 protocols using ivos 2 system

Sperm Analysis via CASA System

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Sperm were extracted and incubated in human tubal fluid (HTF) medium (EasyCheck, Nanjing, China) at 37°C. Sperm samples were diluted and a 10‐μl aliquot of the sperm sample was evenly distributed on a glass chamber slide and analysed using a Computer Assisted Sperm Analyzer (CASA) via the IVOS II™ system (Hamilton Thorne, Beverly, MA, USA).

Zhu F., Yan P., Zhang J., Cui Y., Zheng M., Cheng Y., Guo Y., Yang X., Guo X, & Zhu H. (2019). Deficiency of TPPP2, a factor linked to oligoasthenozoospermia, causes subfertility in male mice. Journal of Cellular and Molecular Medicine, 23(4), 2583-2594.

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Murine Sperm Characterization Using CASA

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For animal sperm preparation, the caudal epididymis from adult mice was dissected and cut. Then, the tissues were incubated in human tubal fluid (HTF) medium (EasyCheck, China) at 37 °C. The sperm samples were diluted, and a 10 μL aliquot of each sperm sample was evenly distributed on a glass chamber slide and analyzed using Computer Assisted Sperm Analyzer via the IVOS II™ system (Hamilton Thorne, Beverly, MA, USA).

Zhao H., Zhao T., Yang J., Huang Q., Wu H., Pan Y., Wang H, & Qian Y. (2022). Epimedium protects against dyszoospermia in mice with Pex3 knockout by exerting antioxidant effects and regulating the expression level of P16. Cell Death & Disease, 13(1), 69.

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Epididymal Sperm Motility Analysis

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Sperm was collected from cauda epididymides and incubated with HTF medium (Irvine Scientific, 90125) for 5 min at 37°C. 10 μl of sperm suspension was placed in slide chamber and analysed by IVOS II system (Hamilton Thorne) with default parameters.

Qi M., Sun H., Guo Y., Zhou Y., Gu X., Jin J., Chen X., Wang F., Ma H., Guo X., Chen H, & Shen B. (2021). m6A reader protein YTHDF2 regulates spermatogenesis by timely clearance of phase‐specific transcripts. Cell Proliferation, 55(1), e13164.

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Sperm Motility Analysis via CASA

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Sperm were extracted and incubated in human tubal fluid (HTF) medium (Easycheck, Nanjing, China, M1120) supplemented with 10% foetal bovine serum (FBS) at 37°C. A 10‐μL aliquot of the sperm samples was evenly distributed on a glass chamber slide and analysed using a Computer Assisted Sperm Analyzer (CASA) via the IVOS II system (Hamilton Thorne, Beverly, MA, USA).

Zhu F., Li W., Zhou X., Chen X., Zheng M., Cui Y., Liu X., Guo X, & Zhu H. (2021). PRSS55 plays an important role in the structural differentiation and energy metabolism of sperm and is required for male fertility in mice. Journal of Cellular and Molecular Medicine, 25(4), 2040-2051.

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