Dako envision flex visualization system

Manufactured by Agilent Technologies

Sourced in Denmark

The Dako EnVision Flex + Visualization System is a lab equipment product designed for immunohistochemistry (IHC) and in situ hybridization (ISH) applications. It provides a sensitive and efficient means of detecting target molecules in biological samples, enabling visualization and analysis.

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Lab products found in correlation

Envision flex target retrieval solution high ph, by Agilent Technologies (3 mentions) Flex ihc microscope slides, by Agilent Technologies (3 mentions) Dako autostainer plus, by Agilent Technologies (2 mentions) Envision flex target retrieval solution, by Agilent Technologies (1 mentions) Autostainer plus, by Agilent Technologies (1 mentions) Ab53212, by Abcam (1 mentions) Autostainer plus, by Abcam (1 mentions)

Spelling variants of this product

Envision System (550 citations) EnVision FLEX (142 citations) Dako Envision system (132 citations) EnVision FLEX system (59 citations) EnVision FLEX visualization system (26 citations) Dako EnVision Flex (10 citations) Envision + visualization system (8 citations) Dako EnVision Flex + System (7 citations) FLEX visualization system (3 citations) Dako EnVision™ visualization system (2 citations)

4 protocols using dako envision flex visualization system

Immunohistochemical Evaluation of SOX2 Expression

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The formalin-fixed, paraffin-embedded tissues were cut into 3-µm sections and dried on Flex IHC microscope slides (Dako, Glostrup, Denmark). The sections were deparaffinized with standard xylene and hydrated through graded alcohols into water. Antigen retrieval was performed using Envision Flex Target Retrieval solution, high pH (Dako). Staining was done at room temperature on an automatic staining workstation (Dako Autostainer Plus, Dako, Glostrup, Denmark) with Anti-SOX2 rabbit polyclonal antibody (Merck Millipore # AB5603) at 1:1000 dilution using the Dako EnVision Flex + Visualization System (Dako Autostainer). Counterstaining with hematoxylin was the final step.
SOX2 staining was evaluated as the percentage of cells with nuclear staining in the dysplastic epithelium. The optimal cut-off value for SOX2 staining calculated by ROC analysis using progression to cancer as end-point was 12.5% (Supplementary Figure S2B). Scores were classified as negative or positive staining on the basis of values below or above the cut-off value of 12.5%.

Granda-Díaz R., Menéndez S.T., Pedregal Mallo D., Hermida-Prado F., Rodríguez R., Suárez-Fernández L., Vallina A., Sánchez-Canteli M., Rodríguez A., Fernández-García M.S., Rodrigo J.P, & García-Pedrero J.M. (2019). The Novel Role of SOX2 as an Early Predictor of Cancer Risk in Patients with Laryngeal Precancerous Lesions. Cancers, 11(3), 286.

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Immunohistochemical Analysis of PI3K and SOX2

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Paraffin-embedded tissue samples were cut into 3-µm sections, deparaffinized with standard xylene, and hydrated through graded alcohols into water. Antigen retrieval was performed by heating the sections with Envision Flex Target Retrieval solution, high pH (Dako, Glostrup, Denmark). Staining was carried out at room temperature on an automatic staining workstation (Dako Autostainer Plus, Glostrup, Denmark) with the following primary antibodies: PI3 Kinase p110α rabbit monoclonal antibody at 1:100 dilution (C73F8; Cell Signaling Technology #4249) or anti-SOX2 rabbit polyclonal antibody at 1:1000 dilution (Merck Millipore, #AB5603) using the Dako EnVision Flex + Visualization System (Dako Autostainer, Glostrup, Denmark) and diaminobenzidine chromogen as the substrate. The final step was counterstaining with hematoxylin.

Montoro-Jiménez I., Granda-Díaz R., Menéndez S.T., Prieto-Fernández L., Otero-Rosales M., Álvarez-González M., García-de-la-Fuente V., Rodríguez A., Rodrigo J.P., Álvarez-Teijeiro S., García-Pedrero J.M, & Hermida-Prado F. (2024). Combined PIK3CA and SOX2 Gene Amplification Predicts Laryngeal Cancer Risk beyond Histopathological Grading. International Journal of Molecular Sciences, 25(5), 2695.

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Immunohistochemical Evaluation of HERG1A Expression

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The formalin-fixed, paraffin-embedded tissues were cut into 3-μm sections and dried on Flex IHC microscope slides (Dako). The sections were deparaffinized with standard xylene and hydrated through graded alcohols into water. Antigen retrieval was performed using Envision Flex Target Retrieval solution, high pH (Dako). Staining was done at room temperature on an automatic staining workstation (Dako Autostainer Plus) with rabbit polyclonal anti-HERG1A (NT) antibody (Enzo Life Sciences, Inc.) at 1:250 dilution using the Dako EnVision Flex + Visualization System (Dako Autostainer). Counterstaining with haematoxylin was the final step.
Since HERG1A staining showed a homogeneous distribution, a semiquantitative scoring system based on staining intensity was applied. Immunostaining was scored blinded to clinical data by two independent observers as negative (0), weakly (1+), moderately (2+) or strongly positive (3+). Scores ≥2 were considered as HERG1A-positive expression.

Menéndez S.T., Villaronga M.Á., Rodrigo J.P., Álvarez-Teijeiro S., Urdinguio R.G., Fraga M.F., Suárez C, & García-Pedrero J.M. (2016). HERG1A potassium channel is the predominant isoform in head and neck squamous cell carcinomas: evidence for regulation by epigenetic mechanisms. Scientific Reports, 6, 19666.

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TMEM16A Immunohistochemistry in Tissue Samples

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The formalin-fixed, paraffin-embedded tissues were cut into 3-μm sections and dried on Flex IHC microscope slides (Dako). The sections were deparaffinized with standard xylene and hydrated through graded alcohols into water. Antigen retrieval was performed using Envision Flex Target Retrieval solution, high pH (Dako). Staining was done at room temperature on an automatic staining workstation (Dako Autostainer Plus) with rabbit polyclonal Anti-TMEM16A antibody (Abcam #ab53212) at 1:500 dilution or with rabbit monoclonal Anti-TMEM16A [SP31] antibody (Abcam #ab64085) at 1:100 dilution using the Dako EnVision Flex + Visualization System (Dako Autostainer). Counterstaining with haematoxylin for 1 minute was the final step.
A semiquantitative scoring system based on staining intensity was applied. Immunostaining was scored blinded to clinical data by two independent observers as negative (0), weak to moderately (1+), and strongly positive (2+) based on staining intensity. Scores ≥ 1 were considered as ANO1 positive expression. Cut-off point was determined by survival.

Rodrigo J.P., Menéndez S.T., Hermida-Prado F., Álvarez-Teijeiro S., Villaronga M.Á., Alonso-Durán L., Vallina A., Martínez-Camblor P., Astudillo A., Suárez C, & María García-Pedrero J. (2015). Clinical significance of Anoctamin-1 gene at 11q13 in the development and progression of head and neck squamous cell carcinomas. Scientific Reports, 5, 15698.

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