Triple-negative MDA-MB-231 human breast adenocarcinoma cancer cells were from Caliper (Mechelen, Belgium; catalogue #119369). HER2+ SkBr3 human breast adenocarcinoma cancer cells (catalogue #HTB-30) and triple-negative MDA-MB-436 human breast adenocarcinoma cancer cells (catalogue #HTB-130) were from ATCC (Manassas, VA, USA). All cancer cell lines were originally derived from pleural effusions [24 (link),25 ,26 (link)]. MDA-MB-231 and SkBr3 cells were routinely cultured in DMEM containing 4.5 g/L glucose and GlutaMax (Thermofisher, Erembodegem, Belgium; catalogue #10566016) with 10% FBS, and MDA-MB-436 cells in IMDM containing GlutaMax (Thermofisher; catalogue #31980030) with 20% FBS. All cells were maintained at a subconfluent state in a humidified atmosphere with 95% room air, 5% CO2 at 37 °C. Cell authenticities were routinely verified with a short tandem repeat (STR) test (Eurofins Genomics, Ebersberg, Germany).
Triple negative mda mb 436 human breast adenocarcinoma cancer cells
Manufactured by American Type Culture Collection
Sourced in United States
The Triple-negative MDA-MB-436 human breast adenocarcinoma cancer cells are a well-characterized cell line derived from a breast cancer tumor. This cell line is used for research purposes to study the biology and characteristics of triple-negative breast cancer, a subtype of breast cancer that lacks expression of the estrogen receptor, progesterone receptor, and HER2 protein.
Automatically generated - may contain errors
2 protocols using triple negative mda mb 436 human breast adenocarcinoma cancer cells
1
Characterization of Breast Cancer Cell Lines
2
Breast Cancer Cell Line Characterization
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Triple-negative MDA-MB-231 human breast adenocarcinoma cancer cells were from Caliper (Mechelen, Belgium; catalogue #119369). HER2+ SkBr3 human breast adenocarcinoma cancer cells (catalogue #HTB-30), triple-negative MDA-MB-436 human breast adenocarcinoma cancer cells (catalogue #HTB-130) and MCF10A human nonmalignant breast epithelial cells (catalogue #CRL-10317) were from ATCC (Manassas, VA, USA). All cancer cell lines were originally derived from pleural effusions [27 (link),28 ,29 (link)]. MDA-MB-231 and SkBr3 cells were routinely cultured in DMEM containing 4.5 g/L glucose and GlutaMax (Thermofisher, Erembodegem, Belgium; catalogue #10566016) with 10% FBS; MDA-MB-436 in IMDM containing GlutaMax (Thermofisher; catalogue #31980030) with 20% FBS; and MCF10A in DMEM:F-12 (Thermofisher; catalogue #11320033) with 5% horse serum, 1 mM CaCl2, 10 mM HEPES, 10 µg/mL insulin, 20 ng/mL epithelial growth factor (EGF) and 0.5 µg/mL hydrocortisone. Cells in culture were maintained at a subconfluent state in a humidified atmosphere with 95% room air and 5% CO2, 37 °C. Cell authenticities were routinely verified with a short tandem repeat (STR) test (Eurofins Genomics, Ebersberg, Germany).
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