Cell membrane stability (CMS) was determined using a conductivity meter (Model Sension 5; HACH Company, Loveland, CO, USA). Ten-day-old seedlings grown on 1× MS medium were placed on filter paper saturated with NaCl (150mM) solution. As soon as symptoms of stress appeared (after 6h) in the WT and VT plants, seedlings were removed, rinsed thoroughly, and immersed in 20ml of double-distilled water at room temperature (20±1 °C). After a gap of 2h, the initial conductivity of samples was recorded. Later, samples were boiled for 30min, cooled to room temperature, and the final conductivity was measured. CMS was calculated using the formula: CMS (%)=1–initial electrical conductivity/electrical conductivity after boiling×100.
Model sension 5
Manufactured by HACH
Sourced in United States
The Model Sension 5 is a portable pH meter designed for versatile pH measurement and temperature monitoring. It features a durable and waterproof housing, an easy-to-read LCD display, and automatic temperature compensation for accurate pH readings across a wide range of temperatures.
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3 protocols using model sension 5
1
Cell Membrane Stability Measurement
2
Measuring Cell Membrane Stability in Seedlings
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Ten-day-old seedlings grown on 1X MS medium were placed on filter paper saturated with NaCl (150 mM) solution. As soon as symptoms of stress appeared (appx. after 6 h) in WT and VT plants, the seedlings were removed, rinsed thoroughly and immersed in 20 ml of double distilled water at room temperature (20 ± 1°C). The initial conductivity of samples was recorded after 2 h using a conductivity meter (Model Sension 5; HACH Company, Loveland, CO, USA). Later, samples were boiled for 30 min and, cooled to room temperature, and the final conductivity was measured. CMS was calculated using the formula CMS (%) = 1—initial electrical conductivity/electrical conductivity after boiling × 100 (Mao et al., 2010 (link)).
3
Electrochemical Characterization of Seawater
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Oxidation–reduction potential (ORP) and pH value of the electrolyzing seawaters were measured with pH/mV/ISE meters (Model Sension 4, Hach Co., Loveland, CO, USA) equipped with an ORP and a pH electrode (part # 5779601-003B and 5773597-003B, respectively, Van London Co., Houston, TX, USA). Electrical conductivity was measured with a Conductivity Meter (Model Sension 5, Hach Co.). A total chlorine test kit (Model 16900; Hach Co.; Method 8209, which is based on Iodometric method.) was used to measure total residual chlorine in the electrolyzed seawaters. The assay was verified periodically using a 100 ± 0.05 ppm chlorine standard solution (Orion Research Inc., Beverly, MA, USA). All measurements were done at 29 ± 1°C.
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