Xevo tq xs tandem quadrupole mass spectrometer

Manufactured by Waters Corporation

Sourced in United States

The Xevo TQ-XS tandem quadrupole mass spectrometer is a high-performance analytical instrument designed for quantitative and qualitative analysis. It utilizes two quadrupole mass analyzers in series to provide accurate and sensitive detection of target compounds in complex samples.

Automatically generated - may contain errors

Lab products found in correlation

Cortecs uplc c18 column, by Waters Corporation (1 mentions) Acquity uplc, by Waters Corporation (1 mentions) Uplc h class system, by Waters Corporation (1 mentions) Xevo tq xs system, by Waters Corporation (1 mentions) Acquity ultra performance lc 1 class system, by Waters Corporation (1 mentions) Acquity uplc sample manager, by Waters Corporation (1 mentions) Masslynx nt software version 4, by Waters Corporation (1 mentions)

5 protocols using xevo tq xs tandem quadrupole mass spectrometer

Quantitative Phenolic Profiling in SBP

Check if the same lab product or an alternative is used in the 5 most similar protocols

Determination of phenolic compounds in SBP extracts was carried out on ACQUITY UPLC ultra-high performance liquid chromatography and Waters Xevo TQ-XS tandem quadrupole mass spectrometer (Waters, MA, USA).
Chromatographic separation: A Waters CORTECS UPLC C18 column (100 × 2.1 mm, 1.6 µm, Waters, MA, USA) was used, and the mobile phase consisted of solvent A (0.1% formic acid aqueous solution, v/v) and solvent B (acetonitrile). The column temperature was 40 °C, flow rate was 0.3 mL/min, and the injection volume was 1 µL. Gradient profile was 0–7.0 min, 10%~50% B; 7.0–10.0 min, 50%~80% B; 10.0–10.1 min, 80%~100% B; 10.1–12 min, 100% A; 12–12.1 min, 100%~10% B; 12.1~15.0 min, 100% B.
Mass spectrometry: An electrospray ionization (ESI) source was utilized with both positive and negative ion detection modes (ESI+/ESI−). The ion source temperature was maintained at 150 °C and the capillary voltage was set at 2.0 kV. The desolvation gas flow rate was set at 600 L/h.

Wu D., Yang Z., Li J., Huang H., Xia Q., Ye X, & Liu D. (2024). Optimizing the Solvent Selection of the Ultrasound-Assisted Extraction of Sea Buckthorn (Hippophae rhamnoides L.) Pomace: Phenolic Profiles and Antioxidant Activity. Foods, 13(3), 482.

+ Open protocol

+ Expand

SARS-CoV-2 Peptide Quantification by LC-MS

Check if the same lab product or an alternative is used in the 5 most similar protocols

Targeted LC-MS analysis Immuno-enriched proteotypic, surrogate peptides were resolved, separated and analysed using an ACQUITY ultra performance liquid chromatography (UPLC) I-Class PLUS system coupled to a Xevo TQ-XS Tandem Quadrupole mass spectrometer (Waters Corporation, Manchester, UK), equipped with a Z-spray electrospray ionisation (ESI) source set to positive mode. An ACQUITY UPLC Peptide BEH C18 1.7 µm, 2.1 mm × 50 mm column was fitted to the LC system.
Peptide eluates were analysed as 10 µL injections and chromatographic separation was performed at a constant flow rate of 0.6 mL/min with water/0.1 % formic acid (A) and acetonitrile/0.1 % formic acid (B) mobile phases. A 3.5 min gradient was used: 95 % mobile phase A initial to 0.25 min, to 60 % A at 2.2 min, to 5 % A at 2.3 min until 2.8 min and to 95 % A at 3.15 min. Column temperature was set to 40 °C. For the source parameters; capillary voltage (0.6 kV), desolvation temperature (650 °C), desolvation flow (1000 L/h) and cone flow (150 L/h) were set accordingly. Simultaneous multiple reaction monitoring (MRM) of SARS-CoV-2 peptides AYN, DGI, and ADE and their stable isotope labelled analogues, was performed (Supplementary Table I).

Lane D., Allsopp R., Holmes C.W., Slingsby O.C., Jukes-Jones R., Bird P., Anderson N.L., Razavi M., Yip R., Pearson T.W., Pope M., Khunti K., Doykov I., Hällqvist J., Mills K., Skipp P., Carling R., Ng L., Shaw J., Gupta P, & Jones D.J.L. (2024). A high throughput immuno-affinity mass spectrometry method for detection and quantitation of SARS-CoV-2 nucleoprotein in human saliva and its comparison with RT-PCR, RT-LAMP, and lateral flow rapid antigen test. Clinical chemistry and laboratory medicine, 62(6).

+ Open protocol

+ Expand

LC-MS/MS Analysis of Alkylquinoline Congeners

Check if the same lab product or an alternative is used in the 5 most similar protocols

Separation and detection of AQ standards was carried out on an LC-MS/MS system consisting of Waters H-Class UPLC system and a Waters Xevo TQ-XS tandem quadrupole mass spectrometer (Milford, MA, USA). Chromatographic separation was carried out at a flow rate of 0.4 mL/min using a 2.1 × 100 mm, 2.7 μm Ascentis Express C8 HPLC Column (Supelco; Eden Prairie, MN, USA) maintained at 30 °C. Gradient elution was effected with solvent A composed of 0.1% FA and 200 μM EDTA in H₂O and solvent B composed of 0.1% FA in acetonitrile. The gradient was as follows: 0.0–0.5 min hold at 80% A, 0.5–4.0 min linear gradient from 80% A to 5% A, 4.5–5.0 min hold at 5% A, 5.5–6.0 min linear gradient from 5% A to 80% A, and 6.0–8.0 min hold at 80% A to re-equilibrate the column. Sample injections were 2 μL.
Mass spectrometric analysis was carried out in selected reaction monitoring (SRM) mode. Optimized tuning of the mass spectrometer for SRM was achieved by directly infusing standards dissolved in 1:1 (v/v) acetonitrile and water at a flow rate of 5 μL/min. The electrospray ionization source was operated in positive ion mode with a capillary voltage of 1 kV and maintained source temperature of 550 °C. Cone voltages and collision energy parameters for the various AQ congeners are provided in ESM Table S2. For SRM, dwell time for each transition was 5 milliseconds.

Brewer L.K., Jones J.W., Blackwood C.B., Barbier M., Oglesby-Sherrouse A, & Kane M.A. (2020). Development and bioanalytical method validation of an LC-MS/MS assay for simultaneous quantitation of 2-alkyl-4(1H)-quinolones for application in bacterial cell culture and lung tissue. Analytical and Bioanalytical Chemistry, 412(7), 1521-1534.

+ Open protocol

+ Expand

Amino Acid Quantification by LC-MS/MS

Check if the same lab product or an alternative is used in the 5 most similar protocols

Free amino acids were derivatized using AccQ$Tag reagents and analyzed on an ACQUITY Ultra Performance LC™ I-Class System (Waters, Milford, MA, USA) coupled with a Xevo™ TQ-XS Tandem Quadrupole Mass Spectrometer (Waters, Manchester, UK) tted with an electrospray ionization (ESI) source.
To optimize MRM parameters, AQC derivatized amino acids were combined infused with a LC ow into the mass spectrometer. Free amino acid standards were derivatized as per Salazar et al. (2012); 11 briey 100 mL of 100 mg mL -1 amino acid standard was mixed with 700 mL of 50 mM ammonium acetate buffer (pH 9.3) and 200 mL of AccQ$Tag reagent, and heated at 55 °C for 10 min. Derivatized amino acids were infused at 30 mL min -1 together with a LC ow (combined infusion) of 0.7 mL min -1 using 70 : 30 water : acetonitrile containing 0.2% FA. Xevo TQ-XS System parameters were as follows: positive polarity, 150 °C source temperature, 550 °C desolvation temperature, 1000 L h -1 desolvation gas ow, and capillary voltage of 0.7 kV. IntelliStart™ Application within Waters MassLynx™ 4.2 SCN 1007 Soware (Waters, Manchester, UK) was used to prole optimal cone voltages and collision energies.

Ng D.H.J., Chan L.Y., Fitzner L., Keppler J.K., Ismail S.M., Hird S., Hancock P., Karin S, & Tobias D. (2023). A novel screening method for free non-standard amino acids in human plasma samples using AccQ·Tag reagents and LC-MS/MS. Analytical methods : advancing methods and applications, 15(4).

+ Open protocol

+ Expand

UPLC-MS/MS Analysis of Vitamin K

Check if the same lab product or an alternative is used in the 5 most similar protocols

The UPLC system consisted of the ACQUITY UPLC sample manager and binary solvent manager (Waters, MA, USA). Separations were performed with a VanGuard BEH C18 (1.7 μm) as the precolumn (Waters) and an ACQUITY UPLC BEH C18 (1.7 μm, 2.1×100 mm) Column (Waters) as the main column. Vitamin K1 and menaquinone 7 (as internal standard) were detected with the XEVO TQ-XS tandem quadrupole mass spectrometer (Waters). The sample temperature was kept at 4°C, and the column temperature was kept at 50°C. The mobile phase was a mixture of MilliQ water (solvent A) and liquid chromatography-grade methanol (Nacalai Tesque) (solvent B). The UPLC conditions are shown in Supplementary Table 1 and the mass spectrometer conditions are shown in Supplementary Table 2. Data analyses were performed using MassLynx NT software version 4.1 (Waters).

Ito S.M., Yamanashi Y., Takada T, & Suzuki H. (2019). Clinical Importance of Drug-Drug Interaction Between Warfarin and Prednisolone and Its Potential Mechanism in Relation to the Niemann-Pick C1-Like 1-Mediated Pathway. Circulation journal : official journal of the Japanese Circulation Society, 83(2).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!