No mycoplasma fetal bovine serum

Poly(ethylene glycol)methyl ether (mPEG, M_n = 1 kDa) was acquired from Rapp Polymers.
The dialysis membrane with a molecular weight cutoff (MWCO) of 12,000–14,000
Da was obtained from Spectra/Pro. Dulbecco’s modified Eagle’s
medium (DMEM), phosphate-buffered saline (PBS, pH 7.4), Hoechst 33342,
no-mycoplasma fetal bovine serum (FBS), trypsin–EDTA, penicillin–streptomycin,
and live cell imaging solution were procured from Thermo Fisher. All
other chemicals were provided by Merck. The chemicals used in this
work were utilized without further purification unless stated otherwise.
Ultrapure Milli-Q water (Millipore, 18.2 MΩ·cm) was employed
in this study.

All materials were purchased from Sigma-Aldrich without further purification unless stated otherwise. Poly(ethylene glycol) methyl ether 2K was purchased from Rapp Polymers and freeze-dried before use. 1,4-Dioxane and tetrahydrofuran (THF) were obtained from Biosolve Chimie (Metz, France). Dialysis membranes MWCO (12,000–14,000 Da) were supplied by Spectra/Pro^® (ThermoFisher, Waltham, MA, USA). Wheat germ agglutinin, Alexa Fluor^TM 488 conjugate, calcein-AM, propidium iodide (PI), Dulbecco’s modified eagle medium (DMEM), phosphate-buffered saline (PBS, pH 7.4), no-mycoplasma fetal bovine serum (FBS), trypsin-EDTA and penicillin–streptomycin was purchased from ThermoFisher (Waltham, MA, USA). Ultrapure Milli Q (St. Louis, MI, USA) water (18.2 MΩ·cm) was used in this work.
Information regarding the analytical equipment can be found in the supporting information.