Cells were seeded at 10,000 cells/dish on 35-mm dishes. After 24 h, cells were exposed to the isolated or combined action of UVA and vitamin B6 test compound in PBS. After exposure, cells were placed under media and cultured for another 72 h. Cell numbers at the time of treatment (and 72 h later) were determined using a Z2 Analyzer (Beckman Coulter, Inc., Fullerton, CA, USA). Proliferation was compared with cells that received mock treatment. The same methodology was used to establish IC50 values (drug concentration that induces 50% inhibition of proliferation of treated cells within 72 h exposure ± SD, n = 3) of anti-proliferative potency.
Z2 analyzer
Manufactured by Beckman Coulter
Sourced in United States
The Z2 Analyzer is a cell counting and sizing instrument designed for accurate and reliable particle analysis. It utilizes the Coulter Principle to measure the volume and count of particles suspended in an electrolyte solution, providing precise data on cell populations and size distributions.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using z2 analyzer
1
UVA and Vitamin B6 Cell Proliferation
2
Monocyte Differentiation and Migration
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ly6Chigh monocytes isolated from the blood and spleen were stimulated with 30 ng/ml M-CSF (Peprotech, Rocky Hill, NJ, USA) in normal culture media (RPMI-1640, 10 % FCS, NEAA, 1 % PenStrep) as indicated. Ly6Chigh monocytes isolated from the bone marrow were differentiated into macrophages with 30 ng/ml M-CSF in normal culture media over 7 days and then stimulated with 10 μg/ml human DiI-medium oxidized LDL (Kalen Biomedical, Montgomery Village, MD, USA) for 16 h in a minimal medium (2 % FCS in RPMI without growth factor supplements). Foam cell formation was evaluated by microscopy and flow cytometry. Bone marrow monocytes, isolated with the EasySep Mouse Monocyte Isolation Kit (Stemcell Technologies, Cologne, Germany) according to the manufacturer’s instructions, were allowed to migrate for 3 h in a transwell chamber (2 × 105 cells per well; Costar 6.5 mm Transwell inserts with 5 μm pore membrane, Corning, Kennebunk, ME, USA) stimulated by CCL2 20 ng/ml, CCL5 20 ng/ml (R&D System, Minneapolis, MN, USA) or BSA 20 ng/ml as control. Cells in the lower chambers were quantified with an automated cell counter (Z2 analyzer, Beckman Coulter, Inc., Brea, CA, USA).
3
Cell Proliferation Assay with D2O
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell numbers at the time of D2O addition (day 0) and 72 h later (day 3) were determined using a Z2 Analyzer (Beckman Coulter, Fullerton, CA, USA), and proliferation was compared with cells that received mock treatment following our published procedure [50 (link),53 (link)].
4
Cell Proliferation Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A published standard procedure was followed [45 (link),46 (link)]. Briefly, cells were seeded at 10,000 cells/dish on 35 mm dishes. After 24 h, cells were treated with test compound. Cell number at the time of compound addition and 72 h later was determined using a Z2 Analyzer (Beckman Coulter, Fullerton, CA, USA). Proliferation was compared with cells that received mock treatment.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!