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Dmem culture medium

Manufactured by BasalMedia
Sourced in China, United States

DMEM (Dulbecco's Modified Eagle's Medium) is a commonly used cell culture medium formulation. It provides essential nutrients and growth factors to support the cultivation of various cell types in vitro.

Automatically generated - may contain errors

2 protocols using dmem culture medium

1

Culturing LN229 and T98G Cell Lines

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LN229 and T98G cell lines were obtained from American Type Culture Collection (ATCC) and maintained in DMEM culture medium (BasalMedia, China) containing 5% and 10% fetal bovine serum and 1% penicillin/streptomycin, respectively. The culture temperature was 37 °C, and the CO2 concentration was 5%. The cells came from our laboratory stocks and, after resuscitation, these cells were used for no more than 10 generations.
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2

Immunofluorescence Staining Protocol

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Paraformaldehyde (Aladdin, China), Triton X-100 (Sangon Biotech, China), DAPI (Thermo Fisher Scientific, USA), Cytochalasin D (GlpBio, USA), DMEM culture medium (Basal Media, China), FBS (Yeasen, China), PS (Basal Media, China), trypsin (Yeasen, China), 1640 culture medium (Basal Media, China), microscope (Ti-E, Nikon, Japan), camera (iXon Ultra 897, Andor, UK).
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