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Bx51 compound microscope

Manufactured by Leica

The Leica BX51 is a compound microscope designed for a range of scientific applications. It features a sturdy, ergonomic construction and provides high-quality optical performance with a variety of objectives and eyepieces. The BX51 is capable of brightfield, darkfield, and phase contrast microscopy techniques.

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2 protocols using bx51 compound microscope

1

Morphometric Analysis of Insect Specimens

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Specimens were collected by sweeping, yellow-pan trapping and were dissected and mounted (dorsal side up) in Canada Balsam following the method described by Noyes (1982) (link) or glued to triangular cards. Photographs were taken with a CCD digital camera attached to an Olympus BX51 compound microscope (slide-mounted specimens) and Leica M205C microscope (card-mounted specimens). Slide-mounted specimen measurements were made using an eye-piece reticle with an Olympus CX21 compound microscope. Card-mounted specimen measurements were taken using an eye-piece reticle with a Motic SMZ168-B dissecting microscope. In the descriptions below, measurements/ratio in brackets after measurement/ratio ranges refer to the holotype. The terminology follows Graham (1987) and Gibson et al. (1997) . The following abbreviations are used:
F1–4 flagellomeres 1–4;
POL minimum distance between lateral ocelli;
OOL minimum distance between lateral ocellus and eye margin;
OD largest diameter of a lateral ocellus;
MV marginal vein;
STV stigmal vein;
SMV submarginal vein.
All specimens studied in this paper are deposited in the insect collections of Northeast Forestry University (NEFU) and Yancheng Teachers University (YCTU).
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2

Histological Processing of Biological Samples

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Adults were relaxed in a 1:1 mixture of MgCl2 and FSW for 30-60 min and preserved for histology in 10% buffered formalin for at least 24 h, then post-fixed in Hollande-Bouin's Fixative (Electron Microscopy Sciences, Hatfield, PA) for 24-72 h, rinsed in 70% EtOH until all traces of Bouin's were removed, as assessed by the color of solution (solution exchanged at least once daily for 10 days) and stored in 70% EtOH until processed. Specimens were dehydrated through an EtOH series, cleared with several washes in xylene and embedded in paraffin (56°C, melting point). Slides with serial sections of 7-8 μm thickness were stained with Crandall's polychrome method-a combination of the Mallory, Gomori, Koneff and Gurr-McConail techniques where time in red stain and counter stain were slightly modified to 3 and 4 min, respectively-and mounted using Permount (Electron Microscopy Sciences, Hatfield, PA). Sections were imaged using an Olympus BX51 compound microscope, Leica DFC400 digital camera and Leica Application Suite ver. 3.6 software.
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