Primary human dermal fibroblasts (phDF) were isolated from juvenile foreskin and expanded in culture. 1 million cells were plated in a T175 flask in DMEM (P04-04515, Pan-Biotech, Germany) supplemented with 10% FCS and 1% P/S (DMEM complete). Media was changed every 3 days. After 7 days, the cells were confluent and were either passaged or cryopreserved in DMEM media +10% FCS +10% DMSO. Cells were passaged at least once before any experiment and only used for experiments up to passage 9. PhDF were analyzed by flow cytometry using CD90 antibody conjugated to APC-Vio770 (CD90; 1:50; REA897; 130-114-905, Miltenyi Biotec, Germany).
Rea897
Manufactured by Miltenyi Biotec
Sourced in Germany
The REA897 is a laboratory equipment product from Miltenyi Biotec. It is a device designed for research purposes, but the core function of the REA897 is not available in this unbiased and concise format.
Automatically generated - may contain errors
3 protocols using rea897
1
Primary Human Dermal Fibroblast Isolation and Culture
2
Immunophenotyping of Adipose-Derived Stem Cells
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Surface marker expression was analyzed by flow cytometry. ASCs were used from 4 donors. Cells were incubated at for 4 °C for 15 min in the dark with the following antibodies: CD45 (1:50, REA747), CD73 (1:50, REA804), CD90 (1:50, REA897), CD105 (1:50, REA794) as well as the isotype control (1:50, REA293) (all from Miltenyi Biotec, Germany). Flow cytometry measurement was done on a MACSQuant10 (Miltenyi Biotech) and data was analyzed using FlowLogic (Miltenyi Biotech). A minimum of 10,000 events was analyzed for each sample.
3
Flow Cytometric Characterization of ASCs
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Surface marker expression was analyzed by ow cytometry. ASCs were used from 4 donors. Cells were incubated at for 4°C for 15 minutes in the dark with the following antibodies: CD45 (1:50, REA747), CD73 (1:50, REA804), CD90 (1:50, REA897), CD105 (1:50, REA794) as well as the isotype control (1:50, REA293) (all from Miltenyi Biotec, Germany). Flow cytometry measurement was done on a MACSQuant10 (Miltenyi Biotech) and data was analyzed using FlowLogic (Miltenyi Biotech). A minimum of 10,000 events was analyzed for each sample.
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