After 75 µg/ml ox-LDL treatment for 48 h, HUVECs were harvested and lysed. Then the cell extract was utilized for the determination of malondialdehyde (MDA), lactate dehydrogenase (LDH), and superoxide dismutase (SOD) activity, as per the user’s guidebook of the MDA kit (Beyotime), LDH kit (Beyotime), and SOD kit-WST (Dojindo, Kumamoto, Japan).
Sod kit wst
Manufactured by Dojindo Laboratories
Sourced in Japan
The SOD kit-WST is a laboratory equipment product developed by Dojindo Laboratories. It is designed to measure the activity of superoxide dismutase (SOD), an important antioxidant enzyme, in biological samples. The kit utilizes a water-soluble tetrazolium salt (WST) as the detection reagent, which produces a colored formazan product upon reaction with superoxide radicals.
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Lab products found in correlation
3 protocols using sod kit wst
1
Oxidized LDL Stress Biomarker Assay
2
ROS Detection and Antioxidant Activity
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To detect ROS production, HK-2 cells were harvested and incubated with 5 μM 2ʹ,7ʹ-dichlorodihydrofluorescein diacetate (DCF-DA; Sigma-Aldrich) for 1 h at 37°C, followed by determining the fluorescence intensity at the wavelength 488/525 nm. Then, ROS generation was quantified using FACS-Calibur (BD Biosciences). To measure SOD and LDH activity, the cells were collected, lysed, and then cell extract was obtained. Then, the examination of SOD and LDH activity in cell extract was performed with a SOD kit-WST (Dojindo, Kumamoto, Japan) and LDH kit (Beyotime) based on the manufacturer’s specification [28 ].
3
Quantifying Oxidative Stress in Kidney Cells
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For the measurement of reactive oxygen species (ROS) generation, SV40-MES13 cells were collected after treatment with HG or NG treatment for 24 h, followed by incubation with 10 nM Dichlorofluoresceindiacetate (DCFH-DA, Beyotime, Shanghai, China) for 30 min at 37°C. And then, the samples were assessed under a fluorescence microscope (485nm excitation, 530 nm emission), followed by quantification with a FACS-Calibur (BD Bioscience). For the detection of Lactate Dehydrogenase (LDH) activity and Superoxide Dismutase (SOD) activity, treated SV40-MES13 cells were harvested and lysed, followed by an examination in the cell extract using LDH kit (Beyotime) and a SOD kit-WST (Dojindo, Kumamoto, Japan), in line with the manufacturer's instructions.
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