In vitro cell invasion was assessed using a real-time cell invasion and migration (RT-CIM) assay system (ACEA Biosciences, Inc, San Diego, CA) with a 16-well transwell plate (CIM-plate 16, Roche Diagnostics, Mannheim, Germany). The surfaces of the upper wells were coated in 5% Matrigel (BD BioSciences, Bedford, MA), and 10% FBS-containing PM was added to the lower chambers. Cells in serum-free medium were added to the upper chambers, and the migration of the cells was monitored by the system every 15 minutes for 24 hours. Data analysis was performed by the supplied RTCA 1.2 software (Roche Diagnostics, Mannheim, Germany) supplied with the instrument.
Rt cim assay system
Manufactured by Agilent Technologies
The RT-CIM assay system is a lab equipment product from Agilent Technologies designed for real-time, label-free cell-based analysis. The system utilizes cell-impedance monitoring technology to measure cellular responses in real-time without the need for labels or dyes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rt cim assay system
1
Real-Time Cell Invasion Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
In vitro cell invasion was assessed using a real-time cell invasion and migration (RT-CIM) assay system (ACEA Biosciences, Inc, San Diego, CA) with a 16-well transwell plate (CIM-plate 16, Roche Diagnostics, Mannheim, Germany). The surfaces of the upper wells were coated in 5% Matrigel (BD BioSciences, Bedford, MA), and 10% FBS-containing PM was added to the lower chambers. Cells in serum-free medium were added to the upper chambers, and the migration of the cells was monitored by the system every 15 minutes for 24 hours. Data analysis was performed by the supplied RTCA 1.2 software (Roche Diagnostics, Mannheim, Germany) supplied with the instrument.
+ Expand
In vitro cell invasion was assessed using a real-time cell invasion and migration (RT-CIM) assay system (ACEA Biosciences, Inc, San Diego, CA) with a 16-well transwell plate (CIM-plate 16, Roche Diagnostics, Mannheim, Germany). The surfaces of the upper wells were coated in 5% Matrigel (BD BioSciences, Bedford, MA), and 10% FBS-containing PM was added to the lower chambers. Cells in serum-free medium were added to the upper chambers, and the migration of the cells was monitored by the system every 15 minutes for 24 hours. Data analysis was performed by the supplied RTCA 1.2 software (Roche Diagnostics, Mannheim, Germany) supplied with the instrument.
2
Real-Time Invasion Assay of Retinal-Treated Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
In vitro invasion capacity of K7M2 cells treated with retinal was compared to control cells using a real-time cell invasion and migration (RT-CIM) assay system (ACEA Biosciences, Inc.), with a 16-well transwell plate (CIM-plate 16, Roche Diagnostics GmbH). The surface of the wells in the upper chamber was coated with Matrigel (BD BioSciences, Bedford, MA, USA) of different concentrations (2.5%, 5%, and 10%). Serum-containing medium (10% FBS) was added to the wells of the lower chamber. Cells (4 × 104 per well) in serum-free medium were seeded in the upper chamber. The migration of the cells through the Matrigel was monitored by the system every 15 minutes for 24 hours. Data analysis was carried out using RTCA Software 1.2 supplied with the instrument.
+ Expand
In vitro invasion capacity of K7M2 cells treated with retinal was compared to control cells using a real-time cell invasion and migration (RT-CIM) assay system (ACEA Biosciences, Inc.), with a 16-well transwell plate (CIM-plate 16, Roche Diagnostics GmbH). The surface of the wells in the upper chamber was coated with Matrigel (BD BioSciences, Bedford, MA, USA) of different concentrations (2.5%, 5%, and 10%). Serum-containing medium (10% FBS) was added to the wells of the lower chamber. Cells (4 × 104 per well) in serum-free medium were seeded in the upper chamber. The migration of the cells through the Matrigel was monitored by the system every 15 minutes for 24 hours. Data analysis was carried out using RTCA Software 1.2 supplied with the instrument.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!